Studies on the ω subunit of Escherichia coli RNA polymerase - Its role in the recovery of denatured enzyme activity

European Journal of Biochemistry

Volumn 247, Issue 3, 1997, Pages 884-889

  Mukherjee, Kakoli ^a   Chatterji, Dipankar ^a  

^a CENTRE FOR CELLULAR AND MOLECULAR BIOLOGY   (India)

Author keywords

Denaturation, reconstitution; Escherichia coli RNA polymerase; subunit; subunit less holoenzyme

Indexed keywords

RNA POLYMERASE;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SUBUNIT; ESCHERICHIA COLI; MUTANT; PRIORITY JOURNAL; PROTEIN DOMAIN;

ESCHERICHIA; ESCHERICHIA COLI;

EID: 0030832404     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00884.x     Document Type: Article

References (42)

1. Backman, K., Ptashne, M. & Gilbert, W. (1976) Construction of plasmids carrying the cl gene of bacteriophage λ, Proc. Natl Acad. Sci. USA 73, 4174-4178.
2. Berg, D. K. (1969) PhD dissertation, University of California, Berkeley.
3. Borukhov, S., Polyakov, A., Nikiforov, V. & Goldfarb, A. (1992) Gre A protein: a transcription elongation factor from Escherichia coli, Proc. Natl Acad. Sci. USA 89, 8899-8902.
4. Burgess, R. R. (1969) Separation and characterization of the subunits of RNA polymerase, J. Biol. Chem. 244, 6168-6176.
5. Burgess, R. R. & Jendrisak, J. J. (1975) A procedure for the rapid, large-scale purification of Escherichia coli DNA-dependent RNA polymerase involving polymin P precipitation and DNA-cellulose chromatography, Biochemistry 14, 4634-4638.
6. Burgess, R. R. & Travers, A. A. (1970) Escherichia coli RNA polymerase: purification, subunit structure and factor requirements, Fed. Proc. 29, 1164-1169.
7. Burgess, R. R., Travers, A. A., Dunn, J. J. & Bautz, E. K. F. (1969) Factor stimulating transcription of RNA polymerase, Nature 221, 43-46.
8. Carpousis, A. J. & Gralla, J. D. (1980) Cycling of ribonucleic acid polymerase to produce oligonucleotides during initiation in vitro at the lacUV5 promoter. Biochemistry 19, 3245-3253.
9. Dennis, P. P. & Nomura, M. (1974) Stringent control of ribosomal protein gene expression in Escherichia coli, Proc. Natl Acad. Sci. USA 71, 3819-3823.
10. Dynan, W. S., Jendrisak, J. J. & Burgess, R. R. (1977) Templates for eukaryotic RNA polymerase II: artifacts can produce an apparent preference for denatured DNA over native DNA, Anal. Biochem. 79, 181-189.
11. Gentry, D. R. & Burgess, R. R. (1986) The cloning and sequence of the gene encoding the omega subunit of Escherichia coli RNA polymerase, Gene (Amst.) 48, 33-40.
12. Gentry, D. R. & Burgess, R. R. (1989) rpoZ, encoding the omega subunit of Escherichia coli RNA polymerase, is in the same operon as spoT, J. Bacteriol. 171, 1271-1277.
13. Gentry, D. R. (1990) PhD dissertation, University of Wisconsin, Madison.
14. Gentry, P. R. & Burgess, R. R. (1990) Overproduction and purification of the ω-subunit of Escherichia coli RNA polymerase, Protein Expression Purif. 1, 81-86.
15. Gentry, D. R., Xiao, H., Burgess, R. R. & Cashel, M. (1991) The omega subunit of Escherichia coli K-12 RNA polymerase is not required for stringent RNA control in vivo, J. Bacteriol. 173, 3901-3903.
16. Gentry, D. R. & Burgess, R. R. (1993) Cross-linking of Escherichia coli RNA polymerase subunits: identification of the β′ as the binding site of ω, Biochemistry 32, 11 224-11 227.
17. Greenblatt, J. & Li, J. (1981) Interaction of the sigma factor and the nusA gene protein of Escherichia coli with RNA polymerase in the initiation-termination cycle of transcription, Cell 24, 421-428.
18. Hager, D., Jin, D. & Burgess, R. R. (1990) Use of mono Q high-resolution ion exchange chromatography to obtain highly pure and active E. coli RNA polymerase, Biochemistry 29, 7890-7894.
19. Heil, A. & Zillig, W. (1970) Purification of bacterial DNA-dependent RNA polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcription, FEBS Lett. 11, 165-168.
20. Helmann, J. D & Chamberlin, M. (1988) Structure and function of bacterial σ factors, Annu. Rev. Biochem. 57, 839-872.
21. Igarishi, K., Fujita, N. & Ishihama, A. (1989) Promoter selectivity of E. coli RNA polymerase: CO factor is responsible for the ppGpp sensitivity, Nucleic Acids Res. 17, 8755-8765.
22. Igarashi, K. & Ishihama, A. (1991) Bipartite functional map of the Escherichia coli RNA polymerase α subunit: involvement of the C-terminal region in transcription activation by cAMP-CRP, Cell 65, 1015-1022.
23. Ishihama, A. & Saitoh, T. (1979) Subunits of RNA polymerase in function and structure. IX. Regulation of RNA polymerase activity by stringent starvation protein, J. Mol. Biol. 129, 517-530.
24. Johnston, D. E. & McClure, W. R. (1976) Abortive initiation of in vitro RNA synthesis on bacteriophage DNA, in RNA polymerase (Losick, R. & Chamberlin, M., eds) pp. 413-428, Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
25. Kajitani, M. & Ishihama, A. (1983a) Determination of the promoter strength in a mixed transcription system: promoters of lactose, tryptophan and ribosomal protein L10 operons from Escherichia coli, Nucleic Acids Res. 11, 671-686.
26. Kajitani, M. & Ishihama, A. (1983b) Determination of the promoter strength in a mixed transcription system. II. Promoters of ribosomal protein S1 and recA protein operons from Escherichia coli, Nucleic Acids Res. 11, 3873-3888.
27. Kajitani, M. & Ishihama, A. (1984) Promoter selectivity of Escherichia coli RNA polymerase. I. Differential stringent control of the multiple promoters from ribosomal RNA and protein operons, J. Biol. Chem. 259, 1951-1957.
28. Kubori, T. & Shimamoto, N. (1996) A branched pathway in the early stage of transcription by Escherichia coli RNA polymerase, J. Mol. Biol. 256, 449-457.
29. Kumar, K. P. & Chatterji, D. (1988) An improved method for the purification of DNA-dependent Escherichia coli RNA polymerase, J. Biochem. Biophys. Methods 15, 235-240.
30. Lowe, P. A., Hager, D. A. & Burgess, R. R. (1979) Purification and properties of the σ-subunit of Escherichia coli DNA-dependent RNA polymerase, Biochemistry 18, 1344-1352.
31. Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951) Protein measurement with the Folin phenol reagent, J. Biol. Chem. 193, 266-275.
32. Miller, J. H. (1972) Experiments in molecular genetics, Cold Spring Har-bor Laboratory, Cold Spring Harbor NY.
33. Mishra, R. K. & Chatterji, D. (1993) Mechanism of initiation of transcription by Escherichia coli RNA polymerase on supercoiled template, Mol. Microbiol. 8, 507-515.
34. Munson, L. M. & Reznikoff, W. S. (1981) Abortive initiation and long ribonucleic acid synthesis, Biochemistry 20, 2081-2085.
35. Orsini, G., Ouhammouch, M., Le Caer, J.-P. & Brody, E. N. (1993) The asiA gene of bacteriophage T4 codes for the anti-sigma 70 protein, J. Bacteriol. 175, 85-93.
36. Pedersen, S., Skove, J., Kajitani, M. & Ishihama, A. (1984) Transcriptional organization of the rpsA operon of Escherichia coli, Mol. Gen. Genet. 196, 135-140.
37. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular cloning: a laboratory manual, 2nd edn, Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
38. Sarubbi, E., Rudd, K., Xiao, H., Ikehara, K., Kalman, M. & Cashel, M. (1989) Characterization of the spoT gene of Escherichia coli, J. Biol. Chem. 264, 15074-15082.
39. Somers, D. G., Pearson, M. L. & Ingles, C. J. (1975) Isolation and characterization of an α-amanitin-resistant rat myoblast mutant cell line possesing α-amanitin-resistant RNA polymerase II, J. Biol. Chem. 250, 4825-4831.
40. Sparkowski, J. & Das, A. (1990) The nucleotide sequence of greA, a suppresor gene that restores growth of an Escherichia coli RNA polymerase mutant at high temperature, Nucleic Acids Res. 18, 6443.
41. Stevens, A. (1972) New small polypeptides associated with DNA-dependent RNA polymerase of Escherichia coli after infection with bacteriophage T4, Proc. Natl Acad. Sci. USA 69, 603-607.
42. Yoshimura, M., Inokuchi, H. & Ozeki, H. (1984) Identification of transfer RNA suppresors in Escherichia coli. IV. Amber suppresor su'6 a double mutant of a new species of leucine tRNA. J. Mol. Biol. 177, 627-644.