Cyclin A- and cyclin E-Cdk complexes shuttle between the nucleus and the cytoplasm

Molecular Biology of the Cell

Volumn 13, Issue 3, 2002, Pages 1030-1045

  Jackman, Mark ^a   Kubota, Yumiko ^a   Den Elzen, Nicole ^a   Hagting, Anja ^a   Pines, Jonathon ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIN A; CYCLIN DEPENDENT KINASE; CYCLIN E;

ANIMAL CELL; ARTICLE; CELL MEMBRANE TRANSPORT; CENTROSOME; COMPLEX FORMATION; CONTROLLED STUDY; DNA REPLICATION; DNA SYNTHESIS; HUMAN; HYDROPHOBICITY; IMMUNOFLUORESCENCE; MITOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LOCALIZATION; PROTEIN MOTIF; REGULATORY MECHANISM;

ACTIVE TRANSPORT, CELL NUCLEUS; ANTIBIOTICS, ANTIFUNGAL; CDC2-CDC28 KINASES; CELL NUCLEUS; CYCLIN A; CYCLIN B; CYCLIN E; CYCLIN-DEPENDENT KINASE 2; CYCLIN-DEPENDENT KINASES; CYTOPLASM; FATTY ACIDS, UNSATURATED; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; INDICATORS AND REAGENTS; KARYOPHERINS; LUMINESCENT PROTEINS; MACROMOLECULAR SUBSTANCES; NUCLEAR PROTEINS; PHOSPHOPROTEINS; PROTEIN BINDING; PROTEIN-SERINE-THREONINE KINASES; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECOMBINANT FUSION PROTEINS;

ANIMALIA;

EID: 0036201059     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.01-07-0361     Document Type: Article

References (78)

1. Nuclear protein import using digitonin-permeabilized cells
2. Development of baculovirus triple and quadruple expression vectors: Co-expression of three or four bluetongue virus proteins and the synthesis of bluetongue virus-like particles in insect cells
3. Major nucleolar proteins shuttle between nucleus and cytoplasm
4. Reversal of terminal differentiation and control of DNA replication: Cyclin A and Cdk2 specifically localize at subnuclear sites of DNA replication
5. CDC46/MCM5, a yeast protein whose subcellular localization is cell cycle-regulated, is involved in DNA replication at autonomously replicating sequences
6. Turnover of cyclin E by the ubiquitin-proteasome pathway is regulated by Cdk2 binding and cyclin phosphorylation
7. G1 cyclin-dependent kinases are sufficient to initiate DNA synthesis in quiescent human fibroblasts
8. Specialization and targeting of B-type cyclins
9. Cyclin A is destroyed in prometaphase and can delay chromosome alignment and anaphase
10. Mice lacking p21CIP1/WAF1 undergo normal development but are defective in G1 checkpoint control
11. Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization
12. A dominant-negative cyclin D1 mutant prevents nuclear import of cyclin-dependent kinase 4 (CDK4) and its phosphorylation by CDK-activating kinase
13. Nuclear export of human beta-catenin can occur independent of CRM1 and the adenomatous polyposis coli tumor suppressor
14. A new human p34 protein kinase, CDK2, identified by complementation of a cdc28 mutation in Saccharomyces cerevisiae, is a homolog of Xenopus Eg1
15. Reconstitution of biochemically altered nuclear pores: Transport can be eliminated and restored
16. Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores
17. Protein kinases in the control of mitosis: Focus on nucleocytoplasmic trafficking
18. Identification of a novel vertebrate cyclin: Cyclin B3 shares properties with both A- and B-type cyclins
19. Cyclin A is required for the onset of DNA replication in mammalian fibroblasts
20. A novel class of RanGTP binding proteins
21. A 41 amino acid motif in importin-alpha confers binding to importin-beta and hence transit into the nucleus
22. Isolation of a protein that is essential for the first step of nuclear protein import
23. MPF localization is controlled by nuclear export
24. Requirement of Cdk2-cyclin E activity for repeated centrosome reproduction in Xenopus egg extracts
25. Identification of a preinitiation step in DNA replication that is independent of origin recognition complex and cdc6, but dependent on Cdk2
26. The requirements for protein synthesis and degradation, and the control of destruction of cyclins A and B in the meiotic and mitotic cell cycles of the clam embryo
27. Human cyclins B1 and B2 are localized to strikingly different structures: B1 to microtubules, B2 primarily to the Golgi apparatus
28. C1P1
29. Structure of a cyclin A-CDK2 complex
30. Cyclin E controls S phase progression and its down-regulation during Drosophila embryogenesis is required for the arrest of cell proliferation
31. Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32Cdk2 protein kinase subunits
32. Formation and activation of a cyclin E-Cdk2 complex during the G1 phase of the human cell cycle
33. Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family
34. Cyclin/Cdk-dependent initiation of DNA replication in a human cell-free system
35. Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor
36. Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved region
37. Dominant-negative mutants of importin-beta block multiple pathways of import and export through the nuclear pore complex
38. New functional activities for the p21 family of CDK inhibitors
39. Cyclin-dependent kinase control of centrosome duplication
40. Expression and function of Drosophila cyclin A during embryonic cell cycle progression
41. Nuclear localization of vertebrate cyclin A correlates with its ability to form complexes with CDK catalytic subunits
42. Centrosome duplication in mammalian somatic cells requires E2F and Cdk2-cyclin A
43. The K nuclear shuttling domain: A novel signal for nuclear import and nuclear export in the hnRNP K protein
44. Differential modes of nuclear localization signal (NLS) recognition by three distinct classes of NLS receptors
45. Nuclear import of Cdk/cyclin complexes: Identification of distinct mechanisms for import of Cdk2/cyclin E and Cdc2/cyclin B1
46. Induction of S-phase entry by E2F transcription factors depends on their nuclear localization
47. Cloning and characterization of hSRP1 gamma, a tissue-specific nuclear transport factor
48. Targeted disruption of skp2 results in accumulation of cyclin E and p27(Kip1), polyploidy and centrosome overduplication
49. Transport of proteins and RNAs in and out of the nucleus
50. Human cyclin E, a nuclear protein essential for the G1-to-S phase transition
51. Nucleophosmin/B23 is a target of CDK2/cyclin E in centrosome duplication
52. Cyclin A is required at two points in the human cell cycle
53. Phosphorylation of mammalian CDC6 by cyclin A/CDK2 regulates its subcellular localization
54. Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B
55. Human cyclins A and B are differentially located in the cell and undergo cell cycle dependent nuclear transport
56. Kinetic analysis of translocation through nuclear pore complexes
57. Interaction of Xenopus Cdc2-cyclin A1 with the origin recognition complex
58. Human CDC6/Cdc18 associates with Orc1 and cyclin-Cdk and is selectively eliminated from the nucleus at the onset of S phase
59. Distinct modes of cyclin E/cdc2c kinase regulation and S-phase control in mitotic and endoreduplication cycles of Drosophila embryogenesis
60. Substrate recruitment to cyclin-dependent kinase 2 by a multipurpose docking site on cyclin A
61. Cyclin E associates with components of the pre-mRNA splicing machinery in mammalian cells
62. Extracellular signal-dependent nuclear import of Stat1 is mediated by nuclear pore-targeting complex formation with NPI-1, but not Rch1
63. Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells
64. Both cyclin A and cyclin E have S phase promoting (Spf) activity in Xenopus egg extracts
65. Ran-independent nuclear import of cyclin B1-Cdc2 by importin beta
66. The carboxyl terminus of RNA helicase A contains a bidirectional nuclear transport domain
67. Specific phosphorylation of nucleophosmin on Thr199 by cyclin-dependent kinase 2-cyclin E, and its role in centrosome duplication
68. Nuclear export of cyclin B1 and its possible role in the DNA damage-induced G2 checkpoint
69. Isolation of the human Cdk2 gene that encodes the cyclin A and adenovirus E1A-associated p33 kinase
70. E2F activity is regulated by cell cycle-dependent changes in subcellular localization
71. Deletion of the Cul1 gene in mice causes arrest in early embryogenesis and accumulation of cyclin E
72. CRM1- and Ran-independent nuclear export of beta-catenin
73. Culprits in the degradation of cyclin E apprehended
74. Activation of cyclin E/CDK2 is coupled to site-specific autophosphorylation and ubiquitin-dependent degradation of cyclin E
75. Cell cycle-regulated nuclear localization of MCM2 and MCM3, which are required for the initiation of DNA synthesis at chromosomal replication origins in yeast
76. Control of cyclin B1 localization through regulated binding of the nuclear export factor CRM1
77. An indelible lineage marker for Xenopus using a mutated green fluorescent protein
78. Cyclin A is required in S phase in normal epithelial cells