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Biomacromolecules
Volumn 3, Issue 2, 2002, Pages 262-271
A tunable hydrogel for encapsulation and controlled release of bioactive proteins
Author keywords

[No Author keywords available]
Indexed keywords

ACRYLAMIDE DERIVATIVE; BOVINE SERUM ALBUMIN; CATALYTIC ANTIBODY; CHYMOTRYPSIN A; ETHANE; FRUCTOSE BISPHOSPHATE ALDOLASE; IMMUNOGLOBULIN G; LYSOZYME;
EID: 0036200418     PISSN: 15257797     EISSN: None     Source Type: Journal    
DOI: 10.1021/bm010147y     Document Type: Article
Times cited : (44)
References (94)
  • 38
    • 0004287481 scopus 로고
    • DeRoss, D., Kajiwara, K., Osada, Y., Yamaguchi, A., Eds; Plenum Press: New York
    • (1991) Polymer Gels , pp. 289-297
    • Hoffman, A.S.1
  • 62
    • 0034894205 scopus 로고    scopus 로고
    • We reported the use of this cross-linker at the 2000 International Chemical Congress of Pacific Basin Societies. Honolulu, HI, December, of the use of this cross-linker appeared in the literature
    • (2001) Biomacromolecules , vol.2 , pp. 22-24
    • Nagahori, N.1    Nishimura, S.-I.2
  • 69
    • 0008631124 scopus 로고    scopus 로고
    • The polymer thus formed was thoroughly washed before the swelling experiments were carried out and no fluorescence was detected in the washing liquids
  • 70
    • 0008652708 scopus 로고    scopus 로고
    • The excitation wavelength in each case was always 350 nm
  • 73
    • 0008671296 scopus 로고    scopus 로고
    • In the case of lysozyme and BSA, the loading was 11.5 mg of protein/g of gel. For IgG, the loading was 23 mg/g of gel
  • 74
    • 0008659988 scopus 로고    scopus 로고
    • Each point is the mean value of at least two experiments. Control in the first time point interference from the resin is observed, due posibly to traces of unpolymerized N,N-dimethylacrylamide which reacts are already corrected to take this into account
  • 75
    • 0008637394 scopus 로고    scopus 로고
    • The swelling degree of 0.5-16 mol % cross-linked resins range from 15 to 5 mL/g of dry resins, respectively
  • 76
    • 0008669424 scopus 로고    scopus 로고
    • Fluorescence was used as a confirmation method that the released amounts of protein measured by the BCA method correspond to protein released and not to interference from the resin
  • 77
    • 0008649341 scopus 로고    scopus 로고
    • The experiments were performed with hydrogels loaded with 11.5, 38, and 77 mg of protein/g of dry resin. A similar release behavior was observed in all cases
  • 80
    • 0008679287 scopus 로고    scopus 로고
    • The amounts of α-chymotrypsin released to the buffer were measured by the BCA method
  • 81
    • 0008649343 scopus 로고    scopus 로고
    • Control experiments in which unloaded resin was treated with either N-succinyl-L-phenylalanine p-nitroanilide (4) or cynol (6) were performed, and in neither case formation of the products was observed
  • 87
    • 0008652709 scopus 로고    scopus 로고
    • Cynol 6 was added to an aliquot of this supernatant, but no significant catalytic activity was detected

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.