Imaging the electrostatic potential of transmembrane channels: Atomic probe microscopy of OmpF porin

Biophysical Journal

Volumn 82, Issue 3, 2002, Pages 1667-1676

  Philippsen, Ansgar ^a   Im, Wonpil ^b   Engel, Andreas ^a   Schirmer, Tilman ^a   Roux, Benoît ^b   Müller, Daniel J ^c  

^a UNIVERSITY OF BASEL   (Switzerland)

^b WEILL CORNELL MEDICAL COLLEGE   (United States)

^c MAX PLANCK INSTITUTE OF MOLECULAR CELL BIOLOGY AND GENETICS   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN F; PORIN; UNCLASSIFIED DRUG;

ARTICLE; ATOMIC FORCE MICROSCOPY; CALCULATION; COMPUTER SIMULATION; CONCENTRATION PROCESS; ELECTRIC POTENTIAL; ELECTROLYTE BALANCE; ESCHERICHIA COLI; IMAGE ANALYSIS; IONIC STRENGTH; LIPID BILAYER; MATHEMATICAL COMPUTING; MEMBRANE CHANNEL; MEMBRANE STRUCTURE; NONHUMAN; OPTICAL RESOLUTION; PEPTIDE MAPPING; PROTEIN STRUCTURE; QUANTITATIVE ASSAY; REPRODUCIBILITY; STRUCTURE ANALYSIS; SURFACE PROPERTY;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0036195902     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(02)75517-3     Document Type: Article

References (46)

1. Atomic force microscope
2. Measuring electrostatic, van der Waals, and hydration forces in electrolyte solutions with an atomic force microscope
3. Measuring surface forces in aqueous solution with the atomic force microscope
4. Crystal structures explain functional properties of two E. coli porins
5. The vacuolating toxin from Helicobacter pylori forms hexameric pores in lipid bilayers at low pH
6. Imaging crystals, polymers, and processes in water with the atomic force microscope
7. Direct measurements of colloidal forces using an atomic force microscope
8. Crystal structure and functional characterization of OmpK36, the osmoporin of Klebsiella pneumoniae
9. Observing single biomolecules at work with the atomic force microscope
10. High-resolution imaging of native biological sample surfaces using scanning probe microscopy
11. Relative surface charge density mapping with the atomic force microscope
12. Direct in situ structural analysis of recombinant outer membrane proteins expressed in an OmpA-deficient mutant Escherichia coli strain
13. Localization of the lipopolysaccharides in metal-shadowed reconstituted lipid-porin membranes
14. Classical electrostatics in biology and chemistry
15. Continuum solvation model: Electrostatic forces from numerical solutions to the Poisson-Boltzmann equation
16. Grand canonical Monte Carlo-Brownian dynamics algorythm for simulating ion channels
17. Electrostatic properties of two porin channels from Escherichia coli
18. Theory of solution of molecules containing widely separated charges with application to zwiterions
19. Mechanisms of solute transport through outer membrane porins: Burning down the house
20. Structural and functional characterization of OmpF porin mutants selected for larger pore size
21. All-atom empirical potential for molecular modeling and dynamics studies of proteins
22. Electrostatics and diffusion in solution: Simulations with the University of Houston Brownian Dynamics Program
23. The electrostatic properties of membranes
24. Adsorption of biological molecules to a solid support for scanning probe microscopy
25. The height of biomolecules measured with the atomic force microscope depends on electrostatic interactions
26. Mapping flexible protein domains at subnanometer resolution with the AFM
27. Electrostatically balanced subnanometer imaging of biological specimens by atomic force microscopy
28. Roles of electrostatic interaction in proteins
29. Transport proteins in bacteria: Common themes in their design
30. Atomic radii for continuum electrostatics calculations based on molecular dynamics free energy simulations
31. + electrolyte solutions: A correlation of double layer and hydration forces with surface cation exchange properties
32. Mapping local electrostatic forces with the atomic force microscope
33. Ion channels, permeation, and electrostatics: Insight into the function of KcsA
34. The cavity and pore helices in the KcsA K+ channel: Electrostatic stabilization of monovalent cations
35. Implicit solvent models
36. Structural and functional characterization of OmpF porin mutants selected for larger pore size
37. Replacement of the sole histidinyl residue in OmpF porin from E. coli by theronine (H21T) does not affect channel structure and function
38. Reduced surface: An efficient way to compute molecular surfaces
39. Reproducible acquisition of Escherichia coli porin surface topographs by atomic force microscopy
40. Native Escherichia coli OmpF porin surfaces probed by atomic force microscopy
41. General and specific porins from bacterial outer membranes
42. Brownian dynamics simulation of ion flow through porin channels
43. Electrostatic interactions in macromolecules: Theory and application
44. Deformation and height anomaly of soft surfaces studied with an AFM
45. Molecular architecture and electrostatic properties of a bacterial porin