Mutational analysis of the TonB1 energy coupler of Pseudomonas aeruginosa

Journal of Bacteriology

Volumn 184, Issue 6, 2002, Pages 1503-1513

  Zhao, Qixun ^a   Poole, Keith ^a  

^a QUEEN S UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; ASPARTIC ACID; BACTERIAL PROTEIN; GLUTAMIC ACID; GLUTAMINE; IRON; LYSINE; PROLINE; PROTEIN TONB1; TYROSINE; UNCLASSIFIED DRUG; VALINE;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL MEMBRANE; CARBOXY TERMINAL SEQUENCE; CHIMERA; ESCHERICHIA COLI; IRON TRANSPORT; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN FUNCTION; PSEUDOMONAS AERUGINOSA; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BIOLOGICAL TRANSPORT; ENERGY METABOLISM; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENES, BACTERIAL; IRON; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PSEUDOMONAS AERUGINOSA; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0036185882     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.184.6.1503-1513.2002     Document Type: Article

References (76)

1. Characterization of the exbBD operon of Escherichia coli and the role of ExbB and ExbD in TonB function and stability
2. FptA, the Fe(III)-pyochelin receptor of Pseudomonas aeruginosa: A phenolate siderophore receptor homologous to hydroxamate siderophore receptors
3. Functional analysis of a C-terminally altered TonB protein of Escherichia coli
4. TonB: A mediator of iron uptake in bacteria
5. Genetic suppression demonstrates interaction of TonB protein with outer membrane transport proteins in Escherichia coli
6. Specific in vivo labeling of cell surface-exposed protein loops: Reactive cysteines in the predicted gating loop mark a ferrichrome binding site and a ligand-induced conformational change of the Escherichia coli FhuA protein
7. The beta-barrel domain of FhuA Δ5-160 is sufficient for TonB-dependent FhuA activities of Escherichia coli
8. Energy-coupled transport across the outer membrane of Escherichia coli: ExbB binds ExbD and TonB in vitro, and leucine 132 in the periplasmic region and aspartate 25 in the transmembrane region are important for ExbD activity
9. Bacterial solutions to the iron-supply problem
10. Cloning and sequencing of the Klebsiella pneumoniae tonB gene and characterization of Escherichia coli-Klebsiella pneumoniae TonB hybrid proteins
11. Crystal structure of the outer membrane active transporter FepA from Escherichia coli
12. Site-directed disulfide bonding reveals an interaction site between energy-coupling protein TonB and BtuB, the outer membrane cobalamin transporter
13. Crystal structure of the dimeric C-terminal domain of TonB reveals a novel fold
14. Cloning and characterization of the ferric enterobactin receptor gene (pfeA) of Pseudomonas aeruginosa
15. Identification and characterization of the tolQRA genes of Pseudomonas aeruginosa
16. 12 transport mutants of Escherichia coli
17. Import of biopolymers into Escherichia coli: Nucleotide sequences of the exbB and exbD genes are homologous to those of the tolQ and tolR genes
18. Siderophore-mediated iron transport: Crystal structure of FhuA with bound lipopolysaccharide
19. Involvement of ExbB and TonB in transport across the outer membrane of Escherichia coli: Phenotypic complementation of exbB mutants by overexpressed tonB and physical stabilization of TonB by ExbB
20. Bacterial transferrin and lactoferrin receptors
21. In vivo evidence for FhuA outer membrane receptor interaction with the TonB inner membrane protein of Escherichia coli
22. TonB protein of Salmonella typhimurium: A model for signal transduction between membranes
23. 12 and ferric iron transport
24. Signal transfer through three compartments: Transcription initiation of the Escherichia coli ferric citrate transport system from the cell surface
25. Suppression of the btuB451 mutation by mutations in the tonB gene suggests a direct interaction between TonB and TonB-dependent receptor proteins in the outer membrane of Escherichia coli
26. Interactions in the TonB-dependent energy transduction complex: ExbB and ExbD form homomultimers
27. Mapping of mutations affecting pyoverdine production in Pseudomonas aeruginosa
28. In vivo synthesis of the periplasmic domain of TonB inhibits transport through the FecA and FhuA iron siderophore transporters of Escherichia coli
29. Creation of deletion, insertion and substitution mutations using a single pair of primers and PCR
30. Role of the TonB amino terminus in energy transduction between membranes
31. Ligand-specific opening of a gated-porin channel in the outer membrane of living bacteria
32. 12 transport in Escherichia coli: Energy coupling between membranes
33. Membrane topology of the Escherichia coli ExbD protein
34. Topology of the ExbB protein in the cytoplasmic membrane of Escherichia coli
35. A sequence-specific function for the N-terminal signal-like sequence of the TonB protein
36. ExbB acts as a chaperone-like protein to stabilize TonB in the cytoplasm
37. Conversion of the FhuA transport protein into a diffusion channel through the outer membrane of Escherichia coli
38. FhuA barrelcork hybrids are active transporters and receptors
39. Regions of Escherichia coli TonB and FepA proteins essential for in vivo physical interactions
40. Identification of TonB homologs in the family Enterobacteriaceae and evidence for conservation of TonB-dependent energy transduction complexes
41. Protonmotive force, ExbB and ligand-bound FepA drive conformational changes in TonB
42. Partial suppression of an Escherichia coli TonB transmembrane mutation (V17) by a missense mutation in ExbB
43. The conserved proline-rich motif is not essential for energy transduction by Escherichia coli TonB protein
44. TonB protein appears to transduce energy by shuttling between the cytoplasmic membrane and the outer membrane in Escherichia coli
45. A site-directed spin-labeling study of ligand-induced conformational change in the ferric enterobactin receptor, FepA
46. Transmembrane signaling across the ligand-gated FhuA receptor: Crystal structures of free and ferrichrome-bound states reveal allosteric changes
47. Modeling ligand-gated receptor activity
48. Electrophoretic resolution of the major outer membrane protein of Escherichia coli K12 into four bands
49. Nucleotide sequence of the ferrienterochelin receptor FepA in Escherichia coli: Homology among outer membrane receptors that interact with TonB
50. Substrate-induced exposure of an energy-coupling motif of a membrane transporter
51. TonB-dependent iron acquisition: Mechanisms of siderophore-mediated active transport
52. Cell envelope signalling in Escherichia coli: Ligand binding to the ferrichrome-iron receptor FhuA promotes interaction with the energy-transducing protein TonB
53. Characterization of in vitro interactions between a truncated TonB protein from Escherichia coli and the outer membrane receptors FhuA and FepA
54. Ligand-induced conformational change in the ferrichrome-iron receptor of Escherichia coli K-12
55. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants
56. ToxA-deficient mutants of Pseudomonas aeruginosa PA103: Isolation and characterization
57. Pyoverdine-mediated iron transport in Pseudomonas aeruginosa: Involvement of a high-molecular-mass outer membrane protein
58. Cloning and nucleotide sequence analysis of the ferripyoverdine receptor gene fpvA of Pseudomonas aeruginosa
59. The tonB gene of Pseudomonas aeruginosa encodes a novel TonB protein
60. Escherichia coli TonB protein is exported from the cytoplasm without proteolytic cleavage of its amino terminus
61. Analysis of Escherichia coli TonB topology by use of PhoA fusions
62. Formation of a gated channel by a ligand-specific transport protein in the bacterial outer membrane
63. The L-arabinose operon
64. Transport across the outer membrane of Escherichia coli K-12 via the FhuA receptor is regulated by the TonB protein of the cytoplasmic membrane
65. Nucleotide sequence of the colicin B activity gene cba: Consensus pentapeptide among TonB-dependent colicins and receptors
66. Energy transduction between membranes. TonB, a cytoplasmic membrane protein, can be chemically cross-linked in vivo to the outer membrane receptor FepA
67. Evidence for a TonB-dependent energy transduction complex in Escherichia coli
68. Expression of Pseudomonas aeruginosa multidrug efflux pumps MexA-MexB-OprM and MexC-MexD-OprJ in a multidrug-sensitive Escherichia coli strain
69. Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen
70. Activity domains of the TonB protein
71. Bacterial heme sources: The role of heme, hemoprotein receptors and hemophores
72. Contribution of outer membrane efflux protein OprM to antibiotic resistance in Pseudomonas aeruginosa independent of MexAB
73. Influence of the TonB energy-coupling protein on efflux-mediated multidrug resistance in Pseudomonas aeruginosa
74. A second tonB gene in Pseudomonas aeruginosa is linked to the exbB and exbD genes