Megakaryothrombopoiesis during ex vivo expansion of human cord blood CD34+ cells using thrombopoietin

Scandinavian Journal of Immunology

Volumn 55, Issue 1, 2002, Pages 88-95

  Woo, S Y ^a   Kie, J H ^b   Ryu, K H ^a   Moon, H S ^a   Chung, W S ^a   Hwang, D H ^c   Kim, S K ^d   Han, T H ^e   Hahn, M J ^e   Chong, Y H ^a   Park, H K ^a   Seoh, J Y ^a,f  

^a Ewha Womans University   (South Korea)

^b National Health Insurance Corporation   (South Korea)

^c Seoul National University   (South Korea)

^d Inha University School of Medicine   (South Korea)

^e Sungkyunkwan University   (South Korea)

^f Ewha Womans University School of Medicine   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DIPHOSPHATE; CD34 ANTIGEN; CELL DNA; FIBRINOGEN RECEPTOR; MONOCLONAL ANTIBODY; PADGEM PROTEIN; THROMBOPOIETIN;

APOPTOSIS; ARTICLE; CELL MATURATION; CONTROLLED STUDY; DNA CONTENT; ELECTRON MICROSCOPY; ENZYME ACTIVITY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; MEGAKARYOCYTE; NICK END LABELING; PERIODIC ACID SCHIFF STAIN; POLYPLOIDY; PRIORITY JOURNAL; THROMBOCYTOPOIESIS; UMBILICAL CORD BLOOD;

ANTIGENS, CD34; APOPTOSIS; BLOOD PLATELETS; CELL DIFFERENTIATION; FETAL BLOOD; HEMATOPOIESIS; HEMATOPOIETIC STEM CELLS; HUMANS; INFANT, NEWBORN; MEGAKARYOCYTES; MICROSCOPY, ELECTRON; THROMBOPOIETIN;

EID: 0036125427     PISSN: 03009475     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-3083.2002.01031.x     Document Type: Article

References (34)

1. Successful transplantation of HLA-matched and HLA-mismatched umbilical cord blood from unrelated donors: Analysis of engraftment and acute graft-versus-host disease
2. Outcomes among 562 recipients of placental-blood transplants from unrelated donors
3. Placental and/or umbilical cord blood: An alternative source of hematopoietic stem cells for transplantation
4. Delayed engraftment of nonobese diabetic/severe combined immunodeficient mice transplanted with ex vivo-expanded human CD34 (+) cord blood cells
5. Comparison of different methods for separation and ex vivo expansion of cord blood progenitor cells
6. Stimulation of megakaryocytopoiesis and thrombopoiesis by the c-Mpl ligand
7. Cloning and expression of murine thrombopoietin cDNA and stimulation of platelet production in vivo
8. Identification and cloning of a megakaryocyte growth and development factor that is a ligand for the cytokine receptor Mpl
9. In vitro megakaryocytopoietic and thrombopoietic activity of c-mpl ligand (TPO) on purified murine hematopoietic stem cells
10. Purification and biologic characterization of plasma-derived megakaryocyte growth and development factor
11. Recombinant human megakaryocyte growth and development factor (rHuMGDF), a ligand for c-Mpl, produces functional human platelets in vitro
12. The role of megakaryocyte growth and development factor in terminal stages of thrombopoiesis
13. Thrombopoietin primes human platelet aggregation induced by shear stress and by multiple agonists
14. Thrombopoietin, the ligand for the Mpl receptor, synergizes with steel factor and other early acting cytokines in supporting proliferation of primitive hematopoietic progenitors of mice
15. The effect of thrombopoietin on the proliferation and differentiation of murine hematopoietic stem cells
16. Activity of the ligand for c-mpl, thrombopoietin, in early haemopoiesis
17. Extensive amplification and self-renewal of human primitive hematopoietic stem cells from cord blood
18. Distinct patterns of apoptosis in association with modulation of CD44 induced by thrombopoietin and granulocyte-colony stimulating factor during ex vivo expansion of human cord blood CD34+ cells
19. Apoptosis and megakaryocytic differentiation during ex vivo expansion of human cord blood of human cord blood CD34+ cells using thrombopoietin
20. Optimization of a flow cytometric method for the simultaneous measurement of cell surface antigen, DNA content, and in vitro BrdUrd incorporation into normal and malignant hematopoietic cells
21. Detection of activated platelets in whole blood using activation- dependent monoclonal antibodies and flow cytometry
22. Cord blood is better than bone marrow for generating megakaryocyfic progenitor cells
23. Ultrastructural characterization of maturation, platelet release, and senescence of human cultured megakaryocytes
24. Effect of thrombopoietin on the development of megakaryocytes and platelets: An ultrastructural analysis
25. Ultrastructure of platelet formation by human megakaryocytes cultured with the Mpl ligand
26. In vitro senescence and apoptotic cell death of human megakaryocytes
27. Immature megakaryocytes undergo apoptosis in the absence of thrombopoietin
28. Thrombopoietin, the Mpl ligand, is essential for full megakaryocyte development
29. Thrombopoietin (c-mpl ligand) acts synergistically with erythropoietin, stem cell factor, and interleukin-ll to enhance murine megakaryocyte colony growth and increases megakaryocyte ploidy in vitro
30. Effects of thrombopoietin on the proliferation and differentiation of primitive and mature haemopoietic progenitor cells in cord blood
31. Comparative analyses of megakaryocytes derived from cord blood and bone marrow
32. Effects of recombinant human thrombopoietin on megakaryocyte colony formation and megakaryocyte ploidy by human CD34+ cells in a serum-free system
33. The development of human megakaryocytes. III. Development of mature megakaryocytes from highly purified committed progenitors in synthetic culture media and inhibition of thrombopoietin-induced polyploidization by interleukin-3
34. Thrombopoietin requires additional megakaryocyte-active cytokines for optimal ex vivo expansion of megakaryocyte precursor cells