Optimization of a flow cytometric method for the simultaneous measurement of cell surface antigen, DNA content, and in vitro BrdUrd incorporation into normal and malignant hematopoietic cells

Cytometry

Volumn 32, Issue 1, 1998, Pages 28-36

  Holm, Mette ^a   Thomsen, Mette ^a   Høyer, Morten ^a   Hokland, Peter ^a  

^a AARHUS UNIVERSITY HOSPITAL   (Denmark)

Author keywords

Cell cycle analysis; Hematopoiesis; Immunophenotyping; In vitro BrdUrd labeling; Three parameter immunofluorescence flow cytometry

Indexed keywords

MEMBRANE ANTIGEN;

ANTIGEN DETECTION; ARTICLE; CELL CYCLE; DNA DETERMINATION; FLOW CYTOMETRY; HEMATOPOIESIS; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; PRIORITY JOURNAL;

ACUTE DISEASE; ANTIBODIES, MONOCLONAL; ANTIGENS, CD34; BONE MARROW CELLS; BROMODEOXYURIDINE; CELL MEMBRANE PERMEABILITY; COLORING AGENTS; DACTINOMYCIN; DNA; FLOW CYTOMETRY; HLA ANTIGENS; HUMANS; LEUKOCYTES, MONONUCLEAR; MYELODYSPLASTIC SYNDROMES; REPRODUCIBILITY OF RESULTS; TISSUE FIXATION; TUMOR CELLS, CULTURED;

EID: 0031923615     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19980501)32:1<28::AID-CYTO4>3.0.CO;2-B     Document Type: Article

References (41)

1. Aten JA, Bakker PJM, Stap J, Boschman GA, Veenhof CHN: DNA double labelling with lodoUrd and CldUrd for spatial and temporal analysis of cell proliferation and DNA replication. Histochem J 24:251-259, 1992.
2. Bakker PJM, Stap J, Tukker CJ, van Oven CH, Veenhof CHN, Aten J: An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo- and chlorodeoxyuridine incorporated into DNA. Cytometry 12:366-372, 1991.
3. Begg AC, McNally NJ, Shrieve DC, Kärcher H: A method to measure the duration of DNA synthesis and the potential doubling time from a single sample. Cytometry 6:620-626, 1985.
4. Begg AC:, Hofland I: Cell kinetic analysis of mixed populations using three-color fluorescence flow Cytometry. Cytometry 12:445-454, 1991.
5. Bennett JM, Catovsky D, Daniel MT, Landrian G, Galton DAG, Gralnick HR, Sultan C. Proposal for the classification of the myelodysplastic syndromes. Br J Haematol 51:189-199, 1982.
6. Carayon P, Bord A: Identification of DNA-replicating lymphocyte subsets using a new method to label the bromo-deoxyuridine incorporated into the DNA. J Immunol Methods 147:225-230, 1992.
7. Clevengcr CV, Bauer KD, Epstein AL: A method for simultaneous nuclear immunofluorescence and DNA content quantitation using monoclonal antibodies and flow cytometry. Cytometry 6:208-214, 1985.
8. Corver WE, Cornelisse CJ, Fleuren GJ: Simultaneous measurement of two cellular antigens and DNA using fluorescein-isothiocyanate, R-phycoerythrin, and propidium iodide on a standard FACScan. Cytometry 15:117-128, 1994.
9. Darzynkiewicz Z, Robinson JP, Crissman HA (eds): Methods in Cell Biology, Vol 41, Flow Cytometry, Ed 2, Pan A. San Diego, Academic Press, 1994, pp 297-316.
10. Dolbeare F, Gratzner H, Pallavicini MG, Gray JW; Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc Natl Acad Sci USA 80:5573-5577, 1983.
11. Evely RS, Burnett AK: "Differentiation therapy" in the myelodysplastic syndromes. Leuk Lymphoma 4:31-47, 1996.
12. Evenson D, Darzynkiewicz Z, Jost L, Janca F, Ballachey B: Changes in accessibility of DNA to various fluorochromes during spermatogenesis. Cytometry 7:45-53, 1986.
13. Foon KA, Todd RF: Immunologic classification of leukemia and lymphoma. Blood 68:1-31, 1986.
14. Gratzner HG: Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science 218:474-475, 1982.
15. Gray JW (ed): Monoclonal antibodies against bromodeoxyuridine. Cytometry 6:499-662, 1985.
16. Hokland P, Kerndrup G, Griffin JD, Ellegaard J: Analyses of leukocyte differentiation antigens in blood and bone marrow from preleukemia (refractory anemia) patients using monoclonal antibodies. Blood 67:898-902, 1986.
17. Hollander Z, Loken MR: Simultaneous analysis of DNA content and surface antigens in human bone marrow. Cytometry 9:485-490, 1988.
18. Høyer M, Bentzen SM, Salling LN, Overgaard J: Influence of sampling time on assessment on potential doubling time. Cytometry 16:144-151, 1994.
19. Jacobberger JW: Intracellular antigen staining: Quantitative immunofluorescence. Methods Companion Methods Enzymol 2:207-218, 1991.
20. Jensen IM, Hokland P: The proliferative activity of myelopoiesis in myelodysplasia evaluated by multiparameter flow cytometry. Br J Haematol 18:477-482, 1994.
21. Jensen PØ, Larsen J, Larsen JK: Flow cytometric measurements of RNA synthesis based on bromouridine labeling and combined with measurement of DNA content or cell surface antigen. Acta Oncol 32:521-524, 1993.
22. Jensen PØ, Larsen J, Christiansen J, Larsen JK: Flow cytometric measurement of RNA synthesis using bromouridine labelling and bromodeoxyuridine antibodies. Cytometry 14:455-458, 1993.
23. Kristensen JS, Hokland P: Monoclonal antibody ratios in malignant myeloid diseases: Diagnostic and prognostic use in myelodysplastic syndromes. Br J Haematol 74:270-276, 1990.
24. Larsen JK: Measurement of cytoplasmic and nuclear antigens. In: Flow Cytometry-A Practical Approach, Omerod MG (ed). Ed 2. Oxford, Oxford University Press, 1994, pp 93-117.
25. Layton DM, Mufti GJ: Myelodysplastic syndromes: Their history, evolution and relation to acute myeloid leukaemia. Blut 53:423-436, 1986.
26. Matsuoka K, Nomura K, Hishino T: Mutagenic effects of brief exposure to bromodeoxyuridine on mouse FM3A cells. Cell Tissue Kinet. 23:495-503, 1990.
27. Ormerod MG, Kubbies M: Cell cycle analysis of asynchronous cell populations by flow cytometry using bromodeoxyuridine label and Hoechst-propidium iodide stain. Cytometry 13:678-685, 1992.
28. Ormerod MG: Study of cell proliferation and death. In: Ormerod MG (ed). Flow Cytometry. Oxford, BIOS Scientific Publishers Limited, 1994, pp 39-52.
29. Penit C, Vasseur F: Phenotype analysis of cycling and postcycling thymocytes: Evaluation of detection methods for BrdUrd and surface proteins. Cytometry 14:757-763, 1993.
30. Foot M: Cell kinetic analysis using continuous BrdU labelling and bivariate Hoechst 33358/ethidium bromide flow cytometry. In: Ormerod MG (ed) Flow Cytometry-A Practical Approach, Ed 1. Oxford, Oxford University Press, 1990, pp 105-112.
31. Rabinovitch PS, Torres RM, Engel D: Simultaneous cell cycle analysis and two-color surface immunofluorescence using 7-aminoactinomycin D and single laser excitation: Applications to study of cell activation and the cell cycle of murine LY-1 B cells. J Immunol 136:2769-2775, 1986.
32. Schimenti KJ, Jacobberger JW: Fixation of mammalian cells for flow cytometric evaluation of DNA content and nuclear immunofluorescence. Cytometry 13:48-59, 1992.
33. Schutte B, Reynders MMJ, van Assche CLMVJ, Hupperets PSGJ, Bosnian FT, Blijham GH: An improved method for the immunocyto- chemical detection of bromodeoxyuridine labeled nuclei using flow cytometry. Cytometry 8:372-376, 1987.
34. Schmid I, Uittenbogaart CH, Giorgi JV: A gentle fixation and permeahilization method for combined cell surface and intracellular staining with improved precision in DNA quantification. Cytometry 12:279-285, 1991.
35. Schmid I, Krall WJ, Uittenbogaart CH, Braun J, Giorgi JV: Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunoflourescence in single laser flow cytometry. Cytometry 13:204-208, 1992.
36. Shapiro HM: Parameters and probes. In: Shapiro HM (ed). Practical Flow Cytometry, Ed 2. Alan R. Liss, Inc., New York, 1988, pp 130-132.
37. Stokke T, Steen HB: Distinction of leukocyte classed based on chromatin-structure-dependent DNA-binding of 7-aminoactinomycin D. Cytometry 8:576-583, 1987.
38. Toba K, Winton EF, Bray RA: Improved staining method for the simultaneous flow cytofluorometric analyses of DNA content. S-phase fraction, and surface phenotype using single laser instrumentation. Cytometry 13:60-67, 1992.
39. Vanderlaan M, Thomas CB: Characterization of monoclonal antibodies to bromodeoxyuridine. Cytometry 6:501-505, 1985.
40. Vogel W, Autenrieth M, Speit G: Detection of bromodeoxyuridine-incorporation in mammalian chromosomes by a bromodeoxyuridine-antibody. Hum Genet 72:129-132, 1986.
41. Zelenin AV, Poletaev AI, Stapanova NG, Barsky VE, Kolesnikov VA, Nikitin SM, Zhuze AL, Gnutchev NV: 7-amino-actinomycin D as a specific fluorophore for DNA content analyses by laser flow cytometry. Cytometry 5:348-354, 1984.