Apoptosis and megakaryocytic differentiation during ex vivo expansion of human cord blood CD34+ cells using thrombopoietin

British Journal of Haematology

Volumn 113, Issue 2, 2001, Pages 470-478

  Ryu, Kyung Ha ^a,b,c,d   Chun, Susan ^a,b,c,d   Carbonierre, Steve ^a,b,c,d   Im, Seock Ah ^a,b,c,d   Kim, Hyung Lae ^a,b,c,d   Shin, Myeong Heon ^a,b,c,d   Shin, Hee Young ^a,b,c,d   Ahn, Hyo Seop ^a,b,c,d   Woo, So Youn ^a,b,c,d   Seoh, Ju Young ^a,b,c,d   Fraser, John K ^a,b,c,d  

^a Department of Paediatrics ^*  (South Korea)

^b Ewha Womans University School of Medicine   (South Korea)

^c Seoul National University   (South Korea)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Apoptosis; CD34+ cells; Cord blood; Ex vivo expansion; Thrombopoietin

Indexed keywords

CD34 ANTIGEN; CD61 ANTIGEN; HERMES ANTIGEN; THROMBOPOIETIN;

APOPTOSIS; ARTICLE; CELL DIFFERENTIATION; COLONY FORMING UNIT; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IMMUNOPHENOTYPING; MEGAKARYOCYTE; MEGAKARYOPOIESIS; PRIORITY JOURNAL; UMBILICAL CORD BLOOD;

ANTIGENS, CD; ANTIGENS, CD34; ANTIGENS, CD44; APOPTOSIS; CELL DIFFERENTIATION; CELL DIVISION; CELLS, CULTURED; COLONY-FORMING UNITS ASSAY; CULTURE MEDIA, SERUM-FREE; FETAL BLOOD; FLOW CYTOMETRY; HUMANS; IMMUNOHISTOCHEMISTRY; IMMUNOPHENOTYPING; INTEGRIN BETA3; MEGAKARYOCYTES; PLATELET MEMBRANE GLYCOPROTEINS; THROMBOPOIETIN;

EID: 0034995618     PISSN: 00071048     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2141.2001.02762.x     Document Type: Article

References (41)

1. Umbilical cord blood as a source of hematopoietic stem cells: From research to clinical application
2. Identification and cloning of a megakaryocyte growth and development factor that is ligand for the cytokine receptor Mpl
3. Megakaryocytic precursors can be generated ex vivo and safely administered to autologous peripheral blood progenitor cell transplant recipients
4. Ability of early acting cytokines to directly promote survival and suppress apoptosis of human primitive CD34+CD38- bone marrow cells with multilineage potential at the single-cell level: Key role of thrombopoietin
5. Recombinant human megakaryocyte growth and development factor (rHuMGDF), a ligand for c-MPL, produces functional human platelets in vitro
6. Stimulation of megakaryocytopoiesis and thrombopoiesis by the c-Mpl ligand
7. Immunogencicity of thrombopoietin mimetic peptide GW395058 in BALB/c mice and New Zealand white rabbits: Evaluation of the potential for thrombopoietin neutralizing antibody production in man
8. Comparison of multiple assays for kinetic detection of apoptosis in thymocytes exposed to dexamethasone or diethylstilbesterol
9. Ultrastructural characterization of maturation, platelet release, and senescence of human cultured megakaryocytes
10. Cytokine production and function in c-mpl-deficient mice: No physiologic role for interleukin-3 in residual megakaryocyte and platelet production
11. Outcome of cord-blood transplantation from related and unrelated donors
12. Proliferative and differentiative potential of thrombopoietin-responsive precursors: Expression of megakaryocytic and erythroid lineages
13. Thrombopoietin combined with early-acting growth factors effectively expands human hematopoietic progenitor cells in vitro
14. Purification and biologic characterization of plasma-derived megakaryocyte growth and development factor
15. Activity of the ligand for c-mpl, thrombopoietin, in early hematopoiesis
16. Thrombopoietin and hematopoietic stem cell development
17. Thrombopoietin expands erythroid, granulocyte-macrophage, and megakaryocyte progenitor cells in normal and myelosuppressed mice
18. Thrombopoietin, the ligand for the Mpl receptor, synergizes with steel factor and other early acting cytokines in supporting proliferation of primitive hematopoietic progenitors of mice
19. Strategies for phenotyping apoptotic peripheral human lymphocytes comparing ISTN, annexin-V and 7-AAD cytofluorometric staining methods
20. Effects of flt-3 ligand in combination with TPO on the expansion of megakaryocytic progenitors
21. Cloning and expression of murine thrombopoietin cDNA and stimulation of platelet production in vivo
22. Hematopoietic deficiencies in c-mpl and TPO knockout mice
23. Thrombopoietin primes human platelet aggregation induced by shear stress and by multiple agonists
24. The use of 7-amino actinomycin D in identifying apoptosis: Simplicity of use and broad spectrum of application compared with other techniques
25. Extensive amplification and self-renewal of human primitive hematopoietic stem cells from cord blood
26. Engraftment in nonobese diabetic severe combined immunodeficient mice of human CD34+ cord blood cells after ex vivo expansion: Evidence for the amplification and self-renewal of repopulating cells
27. Apoptosis of erythroid precursors under stimulation with thrombopoietin: Contribution to megakaryocytic lineage choice
28. A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry
29. Comparison of different methods for separation and ex vivo expansion of cord blood progenitor cells
30. Distinct patterns of apoptosis in association with modulation of CD44 induced by thrombopoietin and granulocyte-colony stimulating factor during ex vivo expansion of human cord blood CD34+ cells
31. The effect of thrombopoietin on the proliferation and differentiation of murine hematopoietic stem cells
32. Role of c-mpl in early hematopoiesis
33. Cord blood is better than bone marrow for generating megakaryocytic progenitor cells
34. High thrombopoietin production by hematopoietic cells induces a fatal myeloproliferative syndrome in mice
35. Successful transplantation of HLA-matched and HLA-mismatched umbilical cord blood from unrelated donors: Analysis of engraftment and acute graft-versus-host disease
36. A model of myelofibrosis and osteosclerosis in mice induced by overexpressing thrombopoietin (mpl ligand): Reversal of disease by bone marrow transplantation
37. Myeloid differentiation of human cord blood CD34+ cells during ex vivo expansion using thrombopoietin, flt3-ligand, and/or granulocyte-colony stimulating factor
38. Thrombopoietin alone stimulates the early proliferation and survival of human erythroid, myeloid and multipotential progenitors in serum-free culture
39. Thrombopoietin stimulates megakaryopoiesis, myelopoiesis, and expansion of CD34+ progenitor cells from single CD34+Thy1+Lin- primitive progenitor cells
40. In vitro senescence and apoptotic cell death of human megakaryocytes
41. In vitro megakaryocytopoietic and thrombopoietic activity of c-mpl ligand (TPO) on purified murine hematopoietic stem cells