Structural features of interferon-γ aggregation revealed by hydrogen exchange

Protein Science

Volumn 11, Issue 6, 2002, Pages 1340-1352

  Tobler, Scott A ^a   Fernandez, Erik J ^a  

^a University of Virginia   (United States)

Author keywords

Chaotropic salts; Conformational changes; Electrospray mass spectrometry; Hydrogen exchange; Protein aggregation; Recombinant human interferon

Indexed keywords

GAMMA INTERFERON; GUANIDINE; RECOMBINANT GAMMA INTERFERON; THIOCYANATE POTASSIUM; DEUTERIUM; HYDROGEN; INORGANIC SALT; RECOMBINANT PROTEIN;

ALPHA HELIX; ARTICLE; CONFORMATIONAL TRANSITION; HYDROPHOBICITY; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STABILITY; PROTEIN STRUCTURE; PROTON TRANSPORT; THERMODYNAMICS; CHEMISTRY; DIMERIZATION; DRUG EFFECT; ELECTROSPRAY MASS SPECTROMETRY; METABOLISM; PROTEIN DENATURATION; PROTEIN TERTIARY STRUCTURE; SOLUTION AND SOLUBILITY;

DEUTERIUM; DIMERIZATION; HYDROGEN; INTERFERON TYPE II; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SALTS; SOLUTIONS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 0036109430     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.3770102     Document Type: Article

References (61)

1. Rapid formation of a molten globule intermediate in refolding of α-lactalbumin
2. Preferential interactions of proteins with salts in concentrated solutions
3. Defining protein ensembles with native-state NH exchange: Kinetics of interconversion and cooperative units from combined NMR and MS analysis
4. Primary structure effects on peptide group hydrogen exchange
5. Protein stability parameters measured by hydrogen exchange
6. Thermal denaturation of human γ-interferon. A calorimetric and spectroscopic study
7. Instability, unfolding, and aggregation of human lysozyme variants underlying amyloid fibrillogenesis
8. Solubility of different folding conformers of bovine growth hormone
9. Amide hydrogen exchange in a highly denatured state
10. Touring the landscapes: Partially folded proteins examined by hydrogen exchange
11. Probing residue-level unfolding during lysozyme precipitation
12. Breakdown in the relationship between thermal and thermodynamic stability in an interleukin-1-β point mutant modified in a surface loop
13. Hydrogen exchange properties in native and denatured states monitored by mass spectrometry and NMR
14. The development of stable protein formulations: A close look at protein aggregation, deamidation, and oxidation
15. Inclusion bodies and recovery of proteins from the aggregated state
16. Aggregation and denaturation of apomyoglobin in aqueous urea solutions
17. Evidence for a self-associating equilibrium intermediate during folding of human growth hormone
18. Identification of unfolding domains in large proteins by their unfolding rates
19. Rate and equilibrium constants for protein unfolding and refolding determined by hydrogen exchange-mass spectrometry
20. Comparison of continuous and pulsed labeling amide hydrogen exchange/mass spectrometry for studies of protein dynamics
21. Protein structure in the lyophilized state: A hydrogen isotope exchange/NMR study with bovine pancreatic trypsin inhibitor
22. Three-dimensional structure of recombinant human interferon-γ
23. Hydrogen exchange and structural dynamics of proteins and nucleic acids
24. Hydrogen exchange: The modem legacy of Linderstrom-Lang
25. Protein aggregation: Folding aggregates, inclusion bodies and amyloid
26. Use of glass electrodes to measure acidities in D20
27. 1H, 13C, and 15N NMR backbone assignments and secondary structure of human interferon-γ
28. Structural studies on acid unfolding and refolding of recombinant human interferon γ
29. Hydrogen exchange in proteins
30. Formation of a molten globule intermediate early in the kinetic folding pathway of apomyoglobin
31. A transient expansion of the native state precedes aggregation of recombinant human interferon-γ
32. Aggregation of recombinant human interferon γ: Kinetics and structural transitions
33. The effect of benzyl alcohol on recombinant human interferon-γ
34. Renaturation of Escherichia coli tryptophanase after exposure to 8 m urea: Evidence for the existence of nucleation centers
35. Thermodynamic and kinetic aspects of protein conformations in relation to physiological function
36. Conformation changes in proteins
37. How does a protein unfold on a reversed-phase liquid chromatography surface?
38. Ligand binding to proteins: The binding landscape model
39. Detection of transient protein folding populations by mass spectrometry
40. Ph dependence of the reversible and irreversible thermal denaturation of γ interferons
41. A nuclear magnetic resonance study of the hydrogen-exchange behaviour of lysozyme in crystals and solution
42. Physical basis of the stability of the folded conformations of proteins
43. Protein-protein interactions as a means of purification
44. Relative effectiveness of various ions on the solubility and crystal growth of lysozyme
45. Crystallization of basic proteins by ion pairing
46. Structural characterization of folding intermediates in cytochrome c by H-exchange labelling and proton NMR
47. The molecular basis of amyloidosis
48. Probing the non-covalent structure of proteins by amide hydrogen exchange and mass spectrometry
49. Specific aggregation of partially folded polypeptide chains: The molecular basis of inclusion body composition
50. Theory for protein solubilities
51. Protein stability: Electrostatics and compact denatured states
52. Amyloidosis
53. Determination of amide hydrogen exchange rates in peptides by mass spectrometry
54. Tracking lysozyme unfolding kinetics during salt-induced precipitation with hydrogen exchange and mass spectrometry
55. Crystal structure of a complex between interferon-γ and its soluble high-affinity receptor
56. Engineered disulfide bonds in recombinant human interferon-γ: The impact of the N-terminal helix A and the AB-loop on protein stability
57. Circular dichrosim study of nascn effect on conformation of poly-l-lysine
58. Mutations and off-pathway aggregation of proteins
59. Disulfide bonds and thermal-stability in T4 lysozyme
60. Determination of amide hydrogen exchange by mass spectrometry: A new tool for protein structure elucidation