Engineered disulfide bonds in recombinant human interferon-γ: The impact of the N-terminal helix A and the AB-loop on protein stability

Protein Engineering

Volumn 9, Issue 10, 1996, Pages 905-912

  Waschütza, Gero ^a   Li, Volkhart ^a   Schäfer, Thomas ^a   Schomburg, Dietmar ^b   Villmann, Carmen ^a   Zakaria, Hayssam ^a   Otto, Bernd ^a  

^a FRAUNHOFER INSTITUTE FOR INTERFACIAL ENGINEERING AND BIOTECHNOLOGY IGB   (Germany)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

Computer modelling; Disulfide bridges; Interferon; Site directed mutagenesis

Indexed keywords

CYSTEINE; GAMMA INTERFERON; GUANIDINE; HLA DR ANTIGEN; RECOMBINANT PROTEIN;

AMINO TERMINAL SEQUENCE; ANTIGEN EXPRESSION; ANTIVIRAL ACTIVITY; ARTICLE; CIRCULAR DICHROISM; COMPUTER MODEL; CONTROLLED STUDY; COVALENT BOND; DISULFIDE BOND; ENCEPHALOMYOCARDITIS VIRUS; FIBROBLAST; FLUORESCENCE; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN DEGRADATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; STEREOCHEMISTRY; TEMPERATURE; THERMODYNAMICS;

ENCEPHALOMYOCARDITIS VIRUS;

EID: 0029966779     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/9.10.905     Document Type: Article

References (30)

1. Arakawa, T., Horan, T.P., McGinley, M. and Rohde, M.F. (1990) J. Interferon Res., 10, 321-329.
2. Creighton, T.E (1988) BioEssays, 8, 57-63
3. Day, C. Schwartz, B., Li, B.L. and Pestka, S. (1992) J. Interferon Res., 12, 139-143.
4. Ealick, S.E., Cook, W.J., Vijay-Kumar, S., Carson, M., Nagabushan, T.L., Trotta, P.P. and Bugg, C.E. (1991) Science, 252, 698-702.
5. Ellman, G.L. (1959). Arch. Biochem. Biophys., 82, 70-77.
6. Gokhale, R.S., Agarwalla, S., Francis, V.S., Santi, D.V. and Balaram, P. (1994) J. Mol. Biol., 235, 89-94.
7. Günther, G. and Otto, B. (1993) Arzneim.-Forsch./Drug Res., 43, 182-186.
8. Harrison, P.M. and Sternberg, M.J. (1994) J. Mol. Biol., 244, 448-463.
9. Hazes, B. and Dijkstra, B.W. (1988) Protein Engng, 2, 119-125.
10. Hogrefe, H.H., McPhie, P., Bekisz, J.B., Enterline, J.C., Dyer, D., Webb, D., Gerrard, T.L. and Zoon, K. (1989) J. Biol. Chem., 264, 12179-12186.
11. Katz, B.A., and Kossiakoff, A. (1986) J. Biol. Chem., 261, 15480-15485.
12. Lauren, S.L., Arakawa, T., Stoney, K. and Rhode, M.F. (1993) Arch. Biochem. Biophys., 306, 350-353.
13. Lunn, C.A., Fossetta, J., Dalgarno, D., Murgolo, N., Windsor, W., Zavodny, P.J., Narula, S.K. and Lundell, D. (1992) Protein Engng, 5, 253-257.
14. Matsumura, M. and Matthews, B.W. (1991) Methods Enzymol., 202, 336-356.
15. Milburn, V.M., Hassell, A.M., Lambert, M.H., Jordan, S.R., Proudfoot, A.E.I., Graber, P. and Wells, T.N.C. (1993) Nature, 363, 172-176.
16. Mitchinson, C. and Wells, J.A. (1989) Biochemistry, 28, 4807-4815.
17. Pabo, C.O. and Suchanek, E. (1986) Biochemistry, 25, 5987-5991.
18. Perry, L.J. and Wetzel, R. (1984) Science, 226, 555-557.
19. Provencher, S.W. and Glockner, J. (1981) Biochemistry, 20, 33-37.
20. Rubinstein, S., Familletti, P.C. and Pestka, S. (1981) J. Virol., 37, 755-758.
21. Sambrook, J., Fritsch, E. and Maniatis, T., (1989) Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
22. Schomburg, D. and Reichelt, J. (1988) J. Mol. Graphics, 6, 161-168.
23. Slodowski, O., Schöne, B., Böhm, J. and Otto, B. (1991) Eur. J. Biochem., 202, 1133-1140.
24. Sowdhamini, R., Srinivasan, N., Shoichet, B., Santi, D.V., Ramakrishnan, C. and Balaram, P. (1989) Protein Engng, 3, 95-103.
25. Thornton, J.M. (1981) J. Mol. Biol., 151, 261-287.
26. van Gunsteren, W.F. and Berendsen, H.J.C. (1987) BIOMOS. Biomolecular Software. Laboratory of Physical Chemistry, University of Groningen.
27. Villafranca, J.E., Howell, E.E., Voet, D.H., Strobel, M.S., Ogden, R.C., Abelson, J.N. and Kraut, J. (1983) Science, 222, 782-788.
28. Villafranca, J.E., Howell, E.E., Oatley, S.J., Xuong, N.H. and Kraut, J. (1987) Biochemistry, 26, 2182-2189.
29. Walter, M.R., Windsor, W.T., Nagabnushan, T.L., Lundell, D.J., Lunn, C.A., Zauodny, P.J. and Narula, S.K. (1995) Nature, 376, 230-235.
30. Weiner, S.J., Kollman, P.A., Nguyen, D.T. and Case, D.A. (1986) J. Comput. Chem., 7, 230-252.