Membrane association and dimerization of a cysteine-rich, 16-kilodalton polypeptide released from the C-terminal region of the coronavirus infectious bronchitis virus 1a polyprotein

Journal of Virology

Volumn 76, Issue 12, 2002, Pages 6257-6267

  Ng, Lisa F P ^a   Liu, D X ^a  

^a NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE; EPIDERMAL GROWTH FACTOR; POLYPROTEIN; TRITON X 100; VIRUS PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE; CONTROLLED STUDY; CORONAVIRUS; DIMERIZATION; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN PROCESSING; PROTEIN SECRETION; PROTEIN TRANSPORT; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS; VIRUS GENE;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CELL MEMBRANE; CERCOPITHECUS AETHIOPS; COS CELLS; CYSTEINE; DIMERIZATION; EPIDERMAL GROWTH FACTOR; INFECTIOUS BRONCHITIS VIRUS; MOLECULAR SEQUENCE DATA; PEPTIDES; POLYPROTEINS; VERO CELLS; VIRAL PROTEINS;

EID: 0036107970     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.76.12.6257-6267.2002     Document Type: Article

References (45)

1. Identification of a domain required for autoproteolytic cleavage of murine coronavirus gene A polyprotein
2. Identification of the catalytic sites of a papain-like cysteine proteinase of murine coronavirus
3. Characterization of a second cleavage site and demonstration of activity in trans by the papain-like proteinase of the murine coronavirus mouse hepatitis virus strain A59
4. Four proteins processed from the replicase gene polyprotein of mouse hepatitis virus colocalize in the cell periphery and adjacent to sites of virion assembly
5. Mouse hepatitis virus replicase protein complexes are translocated to sites of M protein accumulation in the ERGIC at late times of infection
6. Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus
7. An efficient ribosomal frame-shifting signal in the polymerase-encoding region of the coronavirus IBV
8. Characterization of an efficient coronavirus ribosomal frameshifting signal: Requirement for an RNA pseudoknot
9. Translation and processing of mouse hepatitis virus virion RNA in a cell-free system
10. Translation and processing of MHV-A59 virion RNA in reticulocyte lysates and infected cells
11. The putative helicase of the coronavirus mouse hepatitis virus is processed from the replicase gene polyprotein and localizes in complexes that are active in viral RNA synthesis
12. Determination of the p28 cleavage site recognized by the first papain-like cysteine proteinase of murine coronavirus
13. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase
14. Identification of the polymerase polyprotein products p72 and p65 of the murine coronavirus MHV-JHM
15. Coronavirus genome: Prediction of putative functional domains in the nonstructural polyprotein by comparative amino acid sequence analysis
16. Characterization of a 105-kDa polypeptide encoded in gene 1 of the human coronavirus HCV 229E
17. Proteolytic processing at the amino terminus of human coronavirus 229E gene 1-encoded polyproteins: Identification of a papain-like proteinase and its substrate
18. Identification and subcellular localization of a 41 kDa, polyprotein lab processing product in human coronavirus 229E-infected cells
19. Identification of an ATPase activity associated with a 71-kilodalton polypeptide encoded in gene 1 of the human coronavirus 229E
20. Identification of the murine coronavirus p28 cleavage site
21. Identification of mouse hepatitis virus papain-like proteinase 2 activity
22. A simple method for displaying the hydropathic character of a protein
23. Characterization of the two overlapping papain-like proteinase domains encoded in gene 1 of the coronavirus infectious bronchitis virus and determination of the C-terminal cleavage site of an 87-kDa protein
24. Identification of a novel cleavage activity of the first papain-like proteinase domain encoded by open reading frame 1a of the coronavirus Avian infectious bronchitis virus and characterization of the cleavage products
25. A 100 kilodalton polypeptide encoded by open reading frame (ORF) 1b of the coronavirus infectious bronchitis virus is processed by ORF 1a products
26. Identification, expression, and processing of an 87 kDa polypeptide encoded by ORF 1a of the coronavirus infectious bronchitis virus
27. Characterization and mutational analysis of an ORF-1a-encoding proteinase domain responsible for proteolytic processing of the infectious bronchitis virus 1a/1b polyprotein
28. Proteolytic processing of the coronavirus infectious bronchitis virus 1a polyprotein: Identification of a 10-kilodalton polypeptide and determination of its cleavage sites
29. Proteolytic mapping of the coronavirus infectious bronchitis virus 1b polyprotein: Evidence for the presence of four cleavage sites of the 3C-like proteinase and identification of two novel cleavage products
30. Intracellular and in vitro translated 27-kDa proteins contain the 3C-like proteinase activity of the coronavirus MHV-A59
31. Mouse hepatitis virus 3C-like protease cleaves a 22-kilodalton protein from the open reading frame 1a polyprotein in virus-infected cells and in vitro
32. Identification and characterization of a serine-like proteinase of the murine coronavirus MHV-A59
33. Identification of a 24-kDa polypeptide processed from the coronavirus infectious bronchitis virus 1a polyprotein by the 3C-like proteinase and determination of its cleavage sites
34. Further characterization of the coronavirus infectious bronchitis virus 3C-like proteinase and determination of a new cleavage site
35. Efficient autoproteolytic processing of the MHV-A59 3C-like proteinase from the flanking hydrophobic domains requires membranes
36. Processing of the coronavirus MHV-JHM polymerase polyprotein: Identification of precursors and proteolytic products spanning 400 kilodaltons of ORF1a
37. Colocalization and membrane association of murine hepatitis virus gene 1 products and de novo-synthesized viral RNA in infected cells
38. Mouse hepatitis virus replicase proteins associate with two distinct populations of intracellular membranes
39. Activity of a purified His-tagged 3C-like proteinase from the coronavirus infectious bronchitis virus
40. ORF1a encoded replicase subunits are involved in the membrane association of the arterivirus replication complex
41. Localization of mouse hepatitis virus nonstructural proteins and RNA synthesis indicates a role for late endosomes in viral replication
42. Characterization of a human coronavirus (strain 229E) 3C-like proteinase activity
43. Biosynthesis, purification, and characterization of the human coronavirus 229E 3C-like proteinase
44. Processing of the human coronavirus 229E replicase polyproteins by the virus-encoded 3C-like proteinase: Identification of proteolytic products and cleavage sites common to pp1a and pp1ab
45. The autocatalytic release of a putative RNA virus transcription factor from its polyprotein precursor involved two paralogous papain-like proteases that cleave the same peptide bond