Mouse hepatitis virus 3C-like protease cleaves a 22-kilodalton protein from the open reading frame 1a polyprotein in virus-infected cells and in vitro

Journal of Virology

Volumn 72, Issue 3, 1998, Pages 2265-2271

  Lu, Xiao Tao ^a   Sims, Amy C ^a,b   Denison, Mark R ^a,b  

^a VANDERBILT UNIVERSITY MEDICAL CENTER   (United States)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; PROTEINASE; VIRUS ENZYME;

ARTICLE; MURINE HEPATITIS CORONAVIRUS; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PROCESSING; VIRUS CELL INTERACTION;

EID: 0242280088     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.72.3.2265-2271.1998     Document Type: Article

References (26)

1. Baker, S. C., K. Yokomori, S. Dong, R. Carlisle, A. E. Gorbalenya, E. V. Koonin, and M. M. Lai. 1993. Identification of the catalytic sites of a papain-like cysteine proteinase of murine coronavirus. J. Virol. 67:6056-6063.
2. Bonilla, P. J., A. E. Gorbalenya, and S. R. Weiss. 1994. Mouse hepatitis virus strain A59 RNA polymerase gene ORF 1a: heterogeneity among MHV strains. Virology 198:736-740.
3. Boursnell, M. F. G., T. D. K. Brown, I. J. Foulds, P. F. Green, F. M. Tomley, and M. M. Binns. 1987. Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. J. Gen. Virol. 68:57-77.
4. Breedenbeek, P. J., C. J. Pachuk, A. F. H. Noten, J. Charite, W. Luytjes, S. R. Weiss, and W. J. M. Spaan. 1990. The primary structure and expression of the second open reading frame of the polymerase gene of the coronavirus MHV-A59; a highly conserved polymerase is expressed by an efficient ribosomal frameshifting mechanism. Nucleic Acids Res. 18:1825-1832.
5. Brierley, I., M. E. G. Boursnell, M. M. Binns, B. Billimoria, V. C. Blok, T. D. K. Brown, and S. C. Inglis. 1987. An efficient ribosomal frame-shifting signal in the polymerase-encoding region of the coronavirus IBV. EMBO J. 6:3779-3785.
6. Denison, M. R., S. A. Hughes, and S. R. Weiss. 1995. Identification and characterization of a 65-kDa protein processed from the gene 1 polyprotein of the murine coronavirus MHV-A59. Virology 207:316-320.
7. Denison, M. R., P. W. Zoltick, S. A. Hughes, B. Giangreco, A. L. Olson, S. Perlman, J. L. Leibowitz, and S. R. Weiss. 1992. Intracellular processing of the N-terminal ORF 1a proteins of the coronavirus MHV-A59 requires multiple proteolytic events. Virology 189:274-284.
8. Dougherty, W. G., and B. L. Semler. 1993. Expression of virus-encoded proteinase: functional and structural similarities with cellular enzymes. Microbiol. Rev. 57:781-822.
9. Eleouet, J. F., D. Rasschaert, P. Lambert, L. Levy, P. Vende, and H. Laude. 1995. Complete sequence (20 kilobases) of the polyprotein-encoding gene 1 of transmissible gastroenteritis virus. Virology 206:817-822.
10. Gorbalenya, A. E., E. V. Koonin, A. P. Donchenko, and V. M. Blinov. 1989. Coronavirus genome: prediction of putative functional domains in the non-structural polyprotein by comparative amino acid sequence analysis. Nucleic Acids Res. 17:4847-4861.
11. Herold, J., T. Raabe, P. B. Schelle, and S. G. Siddell. 1993. Nucleotide sequence of the human coronavirus 229E RNA polymerase locus. Virology 195:680-691.
12. Kim, J. C., R. A. Spence, P. F. Currier, X. T. Lu, and M. R. Denison. 1995. Coronavirus protein processing and RNA synthesis is inhibited by the cysteine proteinase inhibitor e64d. Virology 208:1-8.
13. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
14. Lawson, M. A., and B. L. Semler. 1990. Picornavirus protein processing -enzymes, substrates, and genetic regulation. Curr. Top. Microbiol. Immunol. 161:49-80.
15. Lee, H.-J., C.-K. Shieh, A. E. Gorbalenya, E. V. Koonin, N. LaMonica, J. Tuler, A. Bagdzhadhzyan, and M. M. C. Lai. 1991. The complete sequence (22 kilobases) of murine coronavirus gene 1 encoding the putative proteases and RNA polymerase. Virology 180:567-582.
16. Liu, D., H. Xu, and T. Brown. 1997. Proteolytic processing of the coronavirus infectious bronchitis virus 1a polyprotein: identification of a 10-kilodalton polypeptide and determination of its cleavage sites. J. Virol. 71:1814-1820.
17. Liu, D. X., and T. D. K. Brown. 1995. Characterisation and mutational analysis of an ORF 1a-encoding proteinase domain responsible for proteolytic processing of the infectious bronchitis virus 1a/1b polyprotein. Virology 209:420-427.
18. Lu, X. T., Y. Q. Lu, and M. R. Denison. 1996. Intracellular and in vitro translated 27-kDa proteins contain the 3C-like proteinase activity of the coronavirus MHV-A59. Virology 222:375-382.
19. Lu, Y., X. Lu, and M. R. Denison. 1995. Identification and characterization of a serine-like proteinase of the murine coronavirus MHV-A59. J. Virol. 69:3554-3559.
20. Lu, Y. Q., and M. R. Denison. 1997. Determinants of mouse hepatitis virus 3C-like proteinase activity. Virology 230:335-342.
21. Pachuk, C. J., P. J. Breedenbeek, P. W. Zoltick, W. J. M. Spaan, and S. R. Weiss. 1989. Molecular cloning of the gene encoding the putative polymerase of mouse hepatitis coronavirus, strain A59. Virology 171:141-148.
22. Pinon, J., R. Mayreddy, J. Turner, F. Khan, P. Bonilla, and S. Weiss. 1997. Efficient autoproteolytic processing of the MHV-A59 3C-like proteinase from the flanking hydrophobic domains requires membranes. Virology 230: 309-322.
23. Saborio, J. L., S.-S. Pong, and G. Koch. 1974. Selective and reversible inhibition of initiation of protein synthesis in mammalian cells. J. Mol. Biol. 85:195-211.
24. Seybert, A., J. Ziebuhr, and S. G. Siddell. 1997. Expression and characterization of a recombinant murine coronavirus 3C-like proteinase. J. Gen. Virol. 78:71-75.
25. Sims, A. C., X. T. Lu, and M. R. Denison. Unpublished data.
26. Ziebuhr, J., J. Herold, and S. G. Siddell. 1995. Characterization of a human coronavirus (strain 229E) 3C-like proteinase activity. J. Virol. 69:4331-4338.