Identification of mouse hepatitis virus papain-like proteinase 2 activity

Journal of Virology

Volumn 74, Issue 17, 2000, Pages 7911-7921

  Kanjanahaluethai, A ^a   Baker, S C ^a  

^a LOYOLA UNIVERSITY CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PAPAIN; PLASMID DNA; PROTEINASE; PROTEINASE INHIBITOR; RNA DIRECTED RNA POLYMERASE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DNA PURIFICATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME DEGRADATION; GENETIC TRANSFECTION; IMMUNOPRECIPITATION; MOLECULAR CLONING; MURINE HEPATITIS CORONAVIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; VACCINIA VIRUS; VIRUS REPLICATION;

ANIMALS; CELL LINE; CLONING, MOLECULAR; CYSTEINE ENDOPEPTIDASES; CYSTEINE PROTEINASE INHIBITORS; HELA CELLS; HUMANS; LEUCINE; MICE; MURINE HEPATITIS VIRUS; MUTATION; PLAQUE ASSAY; PROTEIN PROCESSING, POST-TRANSLATIONAL; RNA REPLICASE; VIRAL NONSTRUCTURAL PROTEINS;

EID: 0033882187     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.74.17.7911-7921.2000     Document Type: Article

References (45)

1. An in vitro system for the leader-primed transcription of coronavirus mRNAs
2. Identification of a domain required for autoproteolytic cleavage of murine coronavirus gene A polyprotein
3. Identification of the catalytic sites of a papain-like cysteine proteinase of murine coronavirus
4. Mouse hepatitis virus strain A59 RNA polymerase gene ORF 1a: Heterogeneity among MHV strains
5. Characterization of the leader papain-like proteinase of MHV-A59: Identification of a new in vitro cleavage site
6. Characterization of a second cleavage site and demonstration of activity in trans by the papain-like proteinase of the murine coronavirus mouse hepatitis virus strain A59
7. An efficient ribosomal frame-shifting signal in the polymerase-encoding region of the coronavirus IBV
8. Identification and characterization of a 65-kDa protein processed from the gene 1 polyprotein of the murine coronavirus MHV-A59
9. Translation and processing of mouse hepatitis virus virion RNA in a cell-free system
10. Identification of putative polymerase gene product in cells infected with murine coronavirus A59
11. The putative helicase of the coronavirus mouse hepatitis virus is processed from the replicase gene polyprotein and localizes in complexes that are active in viral RNA synthesis
12. Intracellular processing of the N-terminal ORF1a proteins of the coronavirus MHV-A59 requires multiple proteolytic events
13. Determinants of the p28 cleavage site recognized by the first papain-like cysteine proteinase of murine coronavirus
14. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase
15. Murine coronavirus membrane fusion is blocked by modification of thiols buried within the spike protein
16. Identification of the polymerase polyprotein products p72 and p65 of the murine coronavirus MHV-JHM
17. Four proteins processed from the replicase gene polyprotein of mouse hepatitis virus colocalize in the cell periphery and adjacent to sites of virion assembly
18. Putative papain-related thiol proteases of positive-strand RNA viruses: Identification of rubi- and aphthovirus proteinases and delineation of a novel conserved domain associated with proteinases of rubi-, alpha- and coronaviruses
19. Viral cysteine proteinases
20. Structure of the foot-and-mouth disease virus leader protease: A papain-like fold adapted for self-processing and eIF4G recognition
21. Proteolytic processing at the amino terminus of human coronavirus 229E gene 1-encoded polyproteins: Identification of a papain-like proteinase and its substrate
22. 2+-binding finger connecting the two domains of a papain-like fold
23. Utility of mouse cell line DBT for propagation and assay of mouse hepatitis virus
24. Identification of the murine coronavirus p28 cleavage site
25. Coronavirus protein processing and RNA synthesis is inhibited by the cysteine proteinase inhibitor E64d
26. The molecular biology of coronaviruses
27. The complete sequence (22 kilobases) of murine coronavirus gene 1 encoding the putative proteases and RNA polymerase
28. Polypeptide requirements for assembly of functional Sindbis virus replication complexes: A model for the temporal regulation of minus- and plus-strand RNA synthesis
29. Identification of a novel cleavage activity of the first papain-like proteinase domain encoded by open reading frame 1a of the coronavirus Avian infectious bronchitis virus and characterization of the cleavage products
30. The crystal structure of hepatitis C virus NS3 proteinase reveals a trypsin-like fold and a structural zinc binding site
31. Intracellular and in vitro-translated 27-kDa proteins contain the 3C-like proteinase activity of the coronavirus MHV-A59
32. Mouse hepatitis virus 3C-like protease cleaves a 22-kilodalton protein from the open reading frame 1a polyprotein in virus-infected cells and in vitro
33. Determinants of mouse hepatitis virus 3C-like proteinase activity
34. Identification and characterization of a serine-like proteinase of the murine coronavirus MHV-A59
35. Analysis of genomic and intracellular viral RNA and small plaque mutants of mouse hepatitis virus, JHM strain
36. Ammonium acetate protocol for the preparation of plasmid DNA suitable for mammalian cell transfections
37. Processing of the coronavirus MHV-JHM polymerase polyprotein: Identification of precursors and proteolytic products spanning 400 kilodaltons of ORF1a
38. Colocalization and membrane association of murine hepatitis virus gene 1 products and de novo-synthesized viral RNA in infected cells
39. Regulation of Sindbis virus RNA replication: Uncleaved P123 and nsP4 function in minus-strand RNA synthesis, whereas cleaved products from P123 are required for efficient plus-strand RNA synthesis
40. The 2A proteinase of human rhinovirus is a zinc containing enzyme
41. Mutational analyses support a model for the HRV2 2A proteinase
42. The NS3 proteinase domain of hepatitis C virus is a zinc-containing enzyme
43. Enhanced growth of a murine coronavirus in transformed mouse cells
44. Expression of murine coronavirus recombinant papain-like proteinase: Efficient cleavage is dependent on the lengths of both the substrate and the proteinase polypeptides
45. ORF1a-encoded replicase subunits are involved in the membrane association of the arterivirus replication complex