Proteolytic processing of the coronavirus infectious bronchitis virus 1a polyprotein: Identification of a 10-kilodalton polypeptide and determination of its cleavage sites

Journal of Virology

Volumn 71, Issue 3, 1997, Pages 1814-1820

  Liu, D X ^a,b   Xu, H Y ^a   Brown, T D K ^b  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; VIRUS PROTEIN;

ANIMAL CELL; ARTICLE; AVIAN INFECTIOUS BRONCHITIS VIRUS; CONTROLLED STUDY; CORONAVIRUS; GENE EXPRESSION; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; SITE DIRECTED MUTAGENESIS; VERO CELL; VIRUS GENE; VIRUS REPLICATION;

EID: 0031058476     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.71.3.1814-1820.1997     Document Type: Article

References (24)

1. Boursnell, M. E. G., T. D. K. Brown, I. J. Foulds, P. F. Green, F. M. Tomley, and M. M. Binns. 1987. Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. J. Gen. Virol. 68:57-77.
2. Brierley, I., M. E. G. Boursnell, M. M. Binns, B. Bilimoria, V. C. Blok, T. D. K. Brown, and S. C. Inglis. 1987. An efficient ribosomal frame-shifting signal in the polymerase-encoding region of the coronavirus IBV. EMBO. J. 6:3779-3785.
3. Brierley, I., P. Digard, and S. C. Inglis. 1989. Characterization of an efficient coronavirus rihosomal frameshifting signal: requirement for an RNA pseudoknot. Cell 57:537-547.
4. Carroll, A. R., D. J. Rowlands, and B. E. Clarke. 1984. The complete nucleotide sequence of the RNA coding for the primary translation product of foot-and-mouth disease virus. Nucleic Acids Res. 12:2461-2472.
5. Fuerst, T. R., E. G. Niles, F. W. Studier, and B. Moss. 1986. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad Sci. USA 83:8122-8127.
6. Gorbalenya, A. E., E. Y. Koonin, A. P. Donchenko, and V. M. nov. 1989. Coronavirus genome: prediction of putative functional domain the non-structural polyprotein by comparative amino acid sequence annuals. Nucleic Acids Res. 17:4847-4860.
7. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
8. Lai, C. J., B. Zhao, H. Hori, and M. Bray. 1991. Infectious RNA transcribed from stably cloned full-length cDNA of dengue type-4 virus. Proc. Natl. Acad. Sci. USA 88:5139-5143.
9. Lee, H.-J., C.-K. Shieh, A. E. Gorbalenya, E. V. Koonin, N. L. Monica, J. Tuler, A. Bagdzhadzhyan, and M. M. C. Lai. 1991. The complete sequence (22 kilobases) of murine coronavirus gene 1 encoding the putative proteases and RNA polymerase. Virology 180:567-582.
10. Liu, D. X., and T. D. K. Brown. 1995. Characterization and mutational analysis of an ORF-1a-encoding proteinase domain responsible for proteolytic processing of the infectious bronchitis virus 1a/1b polyprotein. Virology 209:420-427.
11. Liu, D. X., and S. C. Inglis. 1991. Association of the infectious bronchitis virus 3C protein with the virion envelope. Virology 185:911-917.
12. Liu, D. X., D. Cavanagh, P. Green, and S. C. Inglis. 1991. A polycistronic mRNA specified by the coronavirus infectious bronchitis virus. Virology 184:531-544.
13. Liu, D. X., I. Brierley, K. W. Tibbles, and T. D. K. Brown. 1994. A 100-kilodalton polypeptide encoded by open reading frame (ORF) 1b of the coronavirus infectious bronchitis virus is processed by ORF 1a products. J. Virol. 68:5772-5780.
14. Liu, D. X., K. W. Tibbles, D. Cavanagh, T. D. K. Brown, and I. Brierley. 1995. Identification, expression and processing of an 87 kDa polypeptide encoded by ORF1a of the coronavirus infectious bronchitis virus. Virology 208:48-57.
15. 14a. Liu, D. X., et al. Unpublished observations.
16. MacFarlane, S. A., C. V. Wallis, S. C. Taylor, M. G. Goulden, K. R. Wood, and J. W. Davies. 1991. Construction and analysis of infectious transcripts synthesized from full-length clones of both genomic RNAs of pea early browning virus. Virology 182:124-129.
17. Palmenberg, A. C. 1990. Proteolytic processing of picornaviral polyprotein. Annu. Rev. Microbiol. 44:603-623.
18. Parks, G. D., J. C. Baker, and A. C. Palmenberg. 1989. Proteolytic cleavage of encephalomyocarditis viral capsid region substrates by precursors to the 3C enzyme. J. Virol. 63:1054-1058.
19. Quillet, L., H. Guilley, G. Jonard, and K. Richards. 1989. In vitro synthesis of biologically active beet necrotic yellow vein virus RNA. Virology 172:293-301.
20. Rice, C. M., A. Grakoui, R. Galler, and T. J. Chambers. 1989. Transcription of infectious yellow fever RNA from full-length cDNA templates produced by in vitro ligation. New Biol. 1:285-296.
21. Skotnicki, M. L., S. W. Ding, A. M. Mackenzie, and A. J. Gibbs. 1993. Infectious eggplant mosaic tymovirus and ononis yellow mosaic tymovirus from cloned cDNA. Arch. Virol. 131:47-60.
22. Stern, D. F., and B. M. Sefton. 1984. Coronavirus multiplication: location of genes for virion proteins on the avian infectious bronchitis virus genome. J. Virol. 50:22-29.
23. Sumiyoshi, H., C. H. Hoke, and D. W. Trent. 1992. Infectious Japanese encephalitis virus RNA can be synthesized from in vitro-tigated cDNA templates. J. Virol. 66:5425-5431.
24. Tibbles, K. W., I. Brierley, D. Cavanagh, and T. D. K. Brown. 1996. Characterization in vitro of an autocatalytic processing activity associated with the predicted 3C-like proteinase domain of the coronavirus avian infectious bronchitis virus. J. Virol. 70:1923-1930.