Identification and subcellular localization of a 41 kDa, polyprotein 1ab processing product in human coronavirus 229E-infected cells

Journal of General Virology

Volumn 78, Issue 11, 1997, Pages 2789-2794

  Heusipp, Gerhard ^a   Grötzinger, Carsten ^a,b   Herold, Jens ^a   Siddell, Stuart G ^a   Ziebuhr, John ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b Charit Campus Benjamin Franklin   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CHYMOTRYPSIN; MONOCLONAL ANTIBODY; PROTEINASE; VIRUS ENZYME; VIRUS PROTEIN;

ARTICLE; CELLULAR DISTRIBUTION; CHEMICAL BOND; CORONAVIRUS; CYTOPLASM; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE MICROSCOPY; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN PROCESSING; VIRUS NUCLEOCAPSID;

CORONAVIRUS; HEPATITIS C VIRUS; HUMAN CORONAVIRUS; HUMAN CORONAVIRUS 229E;

EID: 1842370236     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-78-11-2789     Document Type: Article

References (25)

1. Barco, A. & Carrasco, L. (1995). A human virus protein, human poliovirus protein 2BC, induces membrane proliferation and blocks the exocytic pathway in the yeast Saccharomyces cerevisiae. EMBO Journal 14, 3349-3364.
2. Bienz, K., Egger, D., Troxler, M. & Pasamontes, L. (1990). Structural organization of poliovirus RNA replication is mediated by viral proteins of the P2 genomic region. Journal of Virology 64, 1156-1163.
3. Bienz, K., Egger, D., Pfister, T. & Troxler, M. (1992). Structural and functional characterization of the poliovirus replication complex. Journal of Virology 66, 2740-2747.
4. Chambers, T. J., Hahn, C. S., Galler, R. & Rice, C. M. (1990). Flavivirus genome organization, expression, and replication. Annual Review of Microbiology 44, 649-688.
5. Dubois-Dalq, M., Holmes, K. V. & Rentier, B. (1984). Assembly of Coronaviridae. In Assembly of Enveloped RNA Viruses, pp. 100-119. Wien: Springer-Verlag.
6. Eleouet, J.-F., Rasschaert, D., Lambert, P., Levy, L., Vende, P. & Laude, H. (1995). Complete sequence (20 kilobases) of the polyprotein-encoding gene 1 of transmissible gastroenteritis virus. Virology 206, 817-822.
7. Froshauer, S., Kartenbeck, J. & Helenius, A. (1988). Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes. Journal of Cell Biology 107, 2075-2086.
8. Gorbalenya, A. E. & Snijder, E. J. (1996). Viral cysteine proteinases. Perspectives in Drug Discovery and Design 6, 64-86.
9. Gorbalenya, A. E., Koonin, E. V., Donchenko, A. P. & Blinov, V. M. (1989). Coronavirus genome: prediction of putative functional domains in the non-structural polyprotein by comparative amino acid sequence analysis. Nucleic Acids Research 17, 4847-4861.
10. Grötzinger, C. (1996). Expression des RNA-Polymeraselocus des humanen Coronavirus 229E. PhD thesis, Humboldt University, Berlin.
11. Grötzinger, C., Heusipp, G., Ziebuhr, J., Harms, U., Süss, J. & Siddell, S. G. (1996). Characterization of a 105-kDa polypeptide encoded in gene 1 of the human coronavirus HCV 229E. Virology 222, 227-235.
12. Herold, J. & Siddell, S. G. (1993). An elaborated pseudoknot is required for high frequency frameshifting during translation of HCV 229E polymerase mRNA. Nucleic Acids Research 21, 5838-5842.
13. Herold, J., Raabe, T., Schelle-Prinz, B. & Siddell, S. G. (1993). Nucleotide sequence of the human coronavirus 229E RNA polymerase locus. Virology 195, 680-691.
14. Heusipp, G., Harms, U., Siddell, S. G. & Ziebuhr, J. (1997). Identification of an ATPase activity associated with a 71-kDa polypeptide encoded in gene 1 of the human coronavirus 229E. Journal of Virology (in press).
15. Lee, H.-J., Shieh, C.-K., Gorbalenya, A. E., Koonin, E. V., La Monica, N., Tuler, J., Bagdzhadzhyan, A. & Lai, M. M. C. (1991). The complete sequence (22 kilobases) of murine coronavirus gene 1 encoding the putative proteases and RNA polymerase. Virology 180, 567-582.
16. Liu, D. X. & Brown, T. D. K. (1995). Characterization and mutational analysis of an ORF 1a-encoding proteinase domain responsible for proteolytic processing of the infectious bronchitis virus 1a/1b polyprotein. Virology 209, 420-427.
17. Liu, D. X., Xu, H. Y. & Brown, T. D. K. (1997). Proteolytic processing of the coronavirus infectious bronchitis virus 1a polyprotein : identification of a 10-kilodalton polypeptide and determination of its cleavage sites. Journal of Virology 71, 1814-1820.
18. Lu, Y., Lu, X. & Denison, M. R. (1995). Identification and characterization of a serine-like proteinase of the murine coronavirus MHV-A59. Journal of Virology 69, 3554-3559.
19. Lu, X., Lu, Y. & Denison, M. R. (1996). Intracellular and in vitro-translated 27-kDa proteins contain the 3C-like proteinase activity of the coronavirus MHV-A59. Virology 222, 375-382.
20. Seybert, A., Ziebuhr, J. & Siddell, S. G. (1997). Expression and characterization of a recombinant murine coronavirus 3C-like proteinase. Journal of General Virology 78, 71-75.
21. Tibbles, K. W., Brierley, I., Cavanagh, D. & Brown, T. D. K. (1996). Characterization in vitro of an autocatalytic processing activity associated with the predicted 3C-like proteinase domain of the coronavirus avian infectious bronchitis virus. Journal of Virology 70, 1923-1930.
22. Van Dinten, L. C., Wassenaar, A. L. M., Gorbalenya, A. E., Spaan, W. J. M. & Snijder, E. J. (1996). Processing of the equine arteritis virus replicase ORF1b protein: identification of cleavage products containing the putative viral polymerase and helicase domains. Journal of Virology 70, 6625-6633.
23. Ziebuhr, J. (1995). Expression der 3C-like-Proteinase des humanen Coronavirus HCV 229E. PhD thesis, University of Würzburg, Germany.
24. Ziebuhr, J., Herold, J. & Siddell, S. G. (1995). Characterization of a human coronavirus (strain 229E) 3C-like proteinase activity. Journal of Virology 69, 4331-4338.
25. Ziebuhr, J., Heusipp, G. & Siddell, S. G. (1997). Biosynthesis, purification, and characterization of the human coronavirus 229E 3C-like proteinase. Journal of Virology 71, 3992-3997.