Characterization of the dihydroorotate dehydrogenase as a soluble fumarate reductase in Trypanosoma cruzi

Molecular and Biochemical Parasitology

Volumn 122, Issue 2, 2002, Pages 189-200

  Takashima, Eizo ^a   Inaoka, Daniel Ken ^a   Osanai, Arihiro ^a   Nara, Takeshi ^b   Odaka, Masao ^b   Aoki, Takashi ^b   Inaka, Kozi ^c   Harada, Shigeharu ^d   Kita, Kiyoshi ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b JUNTENDO UNIVERSITY   (Japan)

^c Maruwa Food Industry Co Ltd   (Japan)

^d UNIVERSITY OF TOKYO   (Japan)

Author keywords

Dihydroorotate dehydrogenase; Fumarate reductase; Methylviologen; Succinate production; Trypanosoma cruzi

Indexed keywords

DIHYDROOROTATE DEHYDROGENASE; FUMARATE REDUCTASE; FUMARIC ACID; OROTIC ACID DERIVATIVE; PARAQUAT; PYRIMIDINE; RECOMBINANT ENZYME; SUCCINIC ACID;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; CYTOSOL; ELECTRON TRANSPORT; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME PURIFICATION; GENE CLUSTER; LIGHT SCATTERING; MICROBIAL METABOLISM; NONHUMAN; PRIORITY JOURNAL; PYRIMIDINE SYNTHESIS; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SOLUBILITY; SPECIES COMPARISON; TRYPANOSOMA CRUZI;

ANIMALS; BINDING SITES; CYTOSOL; DIHYDROOROTATE OXIDASE; FUMARATES; KINETICS; OXIDATION-REDUCTION; PARAQUAT; RECOMBINANT PROTEINS; SOLUBILITY; SUCCINATE DEHYDROGENASE; SUCCINATES; TRYPANOSOMA CRUZI;

PROTOZOA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; TRYPANOSOMA; TRYPANOSOMA CRUZI;

EID: 0036067218     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(02)00100-7     Document Type: Article

References (48)

1. Role of complex II in anaerobic respiration of the parasite mitochondria from Ascaris suum and Plasmodium falciparum
2. Cytochromes in the respiratory chain of helminth mitochondria
3. The electron transport chain in anaerobically functioning eukaryotes
4. Stage-specific isoforms of complex II (succinate-ubiquinone oxidoreductase) in mitochondria from the parasitic nematode, Ascaris suum
5. Stage-specific isoforms of Ascaris suum complex. II: The fumarate reductase of the parasitic adult and the succinate dehydrogenase of free-living larvae share a common iron-sulfur subunit
6. An anthelmintic compound, nafuredin, shows selective inhibition of complex I in helminth mitochondria
7. The aerobic fermentation of glucose by Trypanosoma cruzi
8. End products and enzyme levels of aerobic glucose fermentation in trypanosomatids
9. Oxidative metabolism in mammalian and culture forms of Trypanosoma cruzi
10. Inhibition of phosphoenolpyruvate carboxykinase from Trypanosome (schizotrypanum) cruzi epimastigotes by 3-mercaptopicolinic acid: In vitro and in vivo studies
11. Differences in energy metabolism between trypanosomatidae
12. Fumarate reductase and other mitochondrial activities in Trypanosoma cruzi
13. Separation of NADH-fumarate reductase and succinate dehydrogenase activities in Trypanosoma cruzi
14. Inhibition of Trypanosoma cruzi and T. brucei NADH fumarate reductase by benznidazole and anthelmintic imidazole derivatives
15. 556
16. Succinate: Quinone oxidoreductases variations on a conserved theme
17. Novel organization and sequences of five genes encoding all six enzymes for de novo pyrimidine biosynthesis in Trypanosoma cruzi
18. Evolutionary implications of the mosaic pyrimidine-biosynthetic pathway in eukaryotes
19. Active site of dihydroorotate dehydrogenase A from Lactococcus lactis investigated by chemical modification and mutagenesis
20. The crystal structure of the flavin containing enzyme dihydroorotate dehydrogenase A from Lactococcus lactis
21. The crystal structure of Lactococcus lactis dihydroorotate dehydrogenase A complexed with the enzyme reaction product throws light on its enzymatic function
22. Structure of dihydroorotate dehydrogenase B: Electron transfer between two flavin groups bridged by an iron-sulfur cluster
23. Structures of human dihydroorotate dehydrogenase in complex with antiproliferative agents
24. Purification and characterization of dihydroorotate dehydrogenase A from Lactococcus lactis, crystallization and preliminary X-ray diffraction studies of the enzyme
25. Two different dihydroorotate dehydrogenases in Lactococcus lactis
26. Dihydrooxonate is a substrate of dihydroorotate dehydrogenase (DHOD) providing evidence for involvement of cysteine and serine residues in base catalysis
27. Specific inhibition of a family 1A dihydroorotate dehydrogenase by benzoate pyrimidine analogues
28. A second dihydroorotate dehydrogenase (Type A) of the human pathogen Enterococcus faecalis: Expression, purification, and steady-state kinetic mechanism
29. Divergent evolution of pyrimidine biosynthesis between anaerobic and aerobic yeasts
30. Catalytic properties of dihydroorotate dehydrogenase from Saccharomyces cerevisiae: Studies on pH, alternate substrates, and inhibitors
31. The B form of dihydroorotate dehydrogenase from Lactococcus lactis consists of two different subunits, encoded by the pyrDb and pyrK genes, and contains FMN, FAD, and [FeS] redox centers
32. Dihydroorotate dehydrogenase B of Enterococcus faecalis. Characterization and insights into chemical mechanism
33. Dihydroorotate dehydrogenase from Clostridium oroticum is a class 1B enzyme and utilizes a concerted mechanism of catalysis
34. Studies on electron transport and energy-linked reactions using mutants of Escherichia coli
35. Purification and properties of the bovine liver mitochondrial dihydroorotate dehydrogenase
36. Analysis of the kinetic mechanism of the bovine liver mitochondrial dihydroorotate dehydrogenase
37. The activity of Escherichia coli dihydroorotate dehydrogenase is dependent on a conserved loop identified by sequence homology, mutagenesis, and limited proteolysis
38. Insight into the chemistry of flavin reduction and oxidation in Escherichia coli dihydroorotate dehydrogenase obtained by rapid reaction studies
39. Crystallization and preliminary X-ray studies of membrane-associated Escherichia coli dihydroorotate dehydrogenase
40. Purification, characterization and localization of mitochondrial dihydroorotate dehydrogenase in Plasmodium falciparum, human malaria parasite
41. Isolation of mitochondria from Plasmodium falciparum showing dihydroorotate dependent respiration
42. Purification and properties of dihydroorotate oxidase from Crithidia fasciculate and Trypanosoma brucei
43. Conversion of dihydroorotate to orotate in parasitic protozoa
44. Inactivation of Crithidia fasciculate carbamoyl-phosphate synthetase II by the antitumor drug acivicin
45. Determination of protein: A modification of the Lowry method that gives a linear photometric response
46. Inducible membrane-bound L-lactate dehydrogenase from Eschrichia coli
47. Reversible enzyme inhibitors as mechanistic probes
48. Flavoenzymes: Diverse catalysts with recurrent features