Telomerase immortalization of human myometrial cells

Biology of Reproduction

Volumn 67, Issue 2, 2002, Pages 506-514

  Condon, Jennifer ^a   Yin, Su ^a   Mayhew, Bobbie ^a   Word, R Ann ^a   Wright, W E ^a   Shay, J W ^a   Rainey, William E ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Female reproductive tract; Oxytocin; Parturition; Pregnancy; Uterus

Indexed keywords

ALPHA ACTIN; CALCIUM; CALDESMON; CALPONIN; ESTROGEN RECEPTOR ALPHA; ESTROGEN RECEPTOR BETA; MESSENGER RNA; OXYTOCIN; OXYTOCIN RECEPTOR; RNA DIRECTED DNA POLYMERASE; TELOMERASE; BIOLOGICAL MARKER; ESTROGEN;

ARTICLE; CALCIUM CELL LEVEL; CELL CULTURE; CELL DIFFERENTIATION; CELL IMMORTALIZATION; CELL ISOLATION; CELL SURVIVAL; CONCENTRATION RESPONSE; CONTROLLED STUDY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MALIGNANT TRANSFORMATION; MYOMETRIUM; PREGNANCY; PRIORITY JOURNAL; PROTEIN EXPRESSION; RETROVIRUS INFECTION; SENESCENCE; TELOMERE; BIOSYNTHESIS; CELL LINE; CELL SEPARATION; CHEMISTRY; CYTOLOGY; DRUG EFFECT; ENZYMOLOGY; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION REGULATION; GENE VECTOR; GENETICS; IMMUNOBLOTTING; METABOLISM; RETROVIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SMOOTH MUSCLE; UTERUS;

UNIDENTIFIED RETROVIRUS; VECTORS;

BIOLOGICAL MARKERS; CALCIUM; CELL LINE; CELL SEPARATION; ESTROGENS; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENETIC VECTORS; HUMANS; IMMUNOBLOTTING; MUSCLE, SMOOTH; MYOMETRIUM; OXYTOCIN; RETROVIRIDAE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA-DIRECTED DNA POLYMERASE; TELOMERASE; TELOMERE; TRANSFECTION; UTERUS;

EID: 0035992840     PISSN: 00063363     EISSN: None     Source Type: Journal    
DOI: 10.1095/biolreprod67.2.506     Document Type: Article

References (52)

1. Maintenance and characterization of human myometrial smooth muscle cells in monolayer culture
2. The serial cultivation of human diploid cell strains
3. Molecular cytogenetic characterization of del (7q) in two uterine leiomyoma-derived cell lines
4. Myometrial characteristics of the Syrian hamster uterine smooth muscle cell line, SHM
5. Oxytocin-stimulated responses in a pregnant human immortalized myometrial cell line
6. Establishment and characterization of a conditionally immortalized smooth muscle/myometrial-like cell line
7. Isolation, immortalization and initial characterization of uterine cell lines: An in vitro system for the porcine uterus
8. Simian virus 40 large T antigen: The puzzle, the pieces, and the emerging picture
9. Extension of life span by introduction of telomerase into normal human cells
10. Genetic and epigenetic changes in human epithelial cells immortalized by telomerase
11. Absence of cancer-associated changes in human fibroblasts immortalized with telomerase
12. Analysis of genomic integrity and p53-dependent G1 checkpoint in telomerase-induced extended-life span human fibroblasts
13. Human keratinocytes that express hTERT and also bypass a p16 (INK4a)-enforced mechanism that limits life span become immortal yet retain normal growth and differentiation characteristics
14. Telomere maintenance mechanisms and cellular immortalization
15. Telomerase activity in human germline and embryonic tissues and cells
16. Advanced mammalian gene transfer: High titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line
17. Both transcriptional and post transcriptional mechanisms regulate human telomerase template RNA levels
18. Single nucleotide polymorphisms (SNPs) in the estrogen receptor gene and breast cancer susceptibility
19. The complete primary structure of human estrogen estrogen receptor beta (hER beta) and its heterodimerization with ER alpha in vivo and in vitro
20. Structure and expression of a human oxytocin receptor
21. Molecular cloning and sequence analysis of smooth muscle calponin
22. Smoothelin, a novel cytoskeletal protein specific for smooth muscle cells
23. Complete amino acid sequence of the human progesterone receptor deduced from cloned cDNA
24. Function and localization of oxytocin receptors in the reproductive tissue of rams
25. Endothelin increases cytoplasmic calcium and myosin phosphorylation in human myometrium
26. 2+ homeostasis in human myometrial smooth muscle cells
27. 2+ indicators with greatly improved fluorescence properties
28. Chicken ovalbumin upstream promoter-transcription factor interacts with estrogen receptor, binds to estrogen response elements and halfsites, and inhibits estrogen-induced gene expression
29. Subsenescent telomere lengths in fibroblasts immortalized by limiting amounts of telomerase
30. Rat heart - A site of oxytocin production and action
31. Regulation of differentiation of vascular smooth muscle cells
32. Vascular smooth muscle calponin. A novel troponin T-like protein
33. Expression of high and low molecular weight caldesmons during phenotypic modulation of smooth muscle cells
34. Developmental pattern of expression and genomic organization of the calponin-h1 gene. A contractile smooth muscle cell marker
35. Immortalization of primary human smooth muscle cells
36. A comparative molecular analysis of four rat smooth muscle cell lines
37. Functional and growth properties of a myometrial cell line derived from transgenic mice: Effects of estradiol and antiestrogens
38. Targeted expression of SV40 large T-antigen to visceral smooth muscle induces proliferation of contractile smooth muscle cells and results in megacolon
39. Alpha-smooth muscle actin is transiently expressed in embryonic rat cardiac and skeletal muscles
40. Alpha-smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing
41. Rat alveolar myofibroblasts acquire alpha-smooth muscle actin expression during bleomycin-induced pulmonary fibrosis
42. Contractile elements and myosin light chain phosphorylation in myometrial tissue from non-pregnant and pregnant women
43. Smoothelin expression characteristics: Development of a smooth muscle cell in vitro system and identification of a vascular variant
44. Expression of oxytocin receptor in human pregnant myometrium
45. Oxytocin receptor and its messenger ribonucleic acid in human leiomyoma and myometrium
46. Estrogen receptors (ER-alpha/ERbeta) in normal and pathological growth of the human myometrium: Pregnancy and leiomyoma
47. Effect of progesterone and oestradiol on expression of connexin43 in cultured human myometrium cells
48. Tissue differences but limited sex steroid responsiveness of c-fos and c-jun in human fibroids and myometrium
49. Elevated connexin-43 expression in term human myometrium correlates with elevated c-Jun expression and is independent of myometrial estrogen receptors
50. Rapid loss of oestrogen and progesterone receptors in human leiomyoma and myometrial explant cultures
51. Two distinct estrogen-regulated promoters generate transcripts encoding the two functionally different human progesterone receptor forms A and B
52. Distribution of the A and B forms of the progesterone receptor messenger ribonucleic acid and protein in uterine leiomyomata and adjacent myometrium