Organelle pH studies using targeted avidin and fluorescein-biotin

Chemistry and Biology

Volumn 7, Issue 3, 2000, Pages 197-209

  Wu, Minnie M ^a   Llopis, Juan ^b   Adams, Stephen ^b   McCaffery, J Michael ^b,c   Kulomaa, Markku S ^d   Machen, Terry E ^a   Moore, Hsiao Ping H ^a   Tsien, Roger Y ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c JOHNS HOPKINS UNIVERSITY   (United States)

^d UNIVERSITY OF JYVÄSKYLÄ   (Finland)

Author keywords

Flubi 2; Targeted fluorescence

Indexed keywords

MAMMALIA;

EID: 0034057989     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(00)00088-0     Document Type: Article

References (27)

1. Kim J.H., Grinstein S.et al. Noninvasive measurement of the pH of the endoplasmic reticulum at rest and during calcium release. Proc. Natl Acad. Sci. USA. 95:1998;2997-3002.
2. Seksek O., Biwersi J., Verkman A.S. Direct measurement of trans-Golgi pH in living cells and regulation by second messengers. J. Biol. Chem. 270:1995;4967-4970.
3. Kim J.H., Lingwood C.A., Williams D.B., Furuya W., Manolson M.F., Grinstein S. Dynamic measurement of the pH of the Golgi complex in living cells using retrograde transport of the verotoxin receptor. J. Cell Biol. 134:1996;1387-1399.
4. Llopis J., McCaffery J.M., Miyawaki A., Farquhar M.G., Tsien R.Y. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc. Natl Acad. Sci. USA. 95:1998;6803-6808.
5. Miesenböck G., De Angelis D.A., Rothman J.E. Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature. 394:1998;192-195.
6. Mellman I., Fuchs R., Helenius A. Acidification of the endocytic and exocytic pathways. Annu. Rev. Biochem. 55:1986;663-700.
7. Halban P.A., Irminger J.C. Sorting and processing of secretory proteins. Biochem. J. 299:1994;1-18.
8. Schmidt W.K., Moore H.P. Ionic milieu controls the compartment-specific activation of pro-opiomelanocortin processing in AtT-20 cells. Mol. Biol. Cell. 6:1995;1271-1285.
9. Rybak S.L., Lanni F., Murphy R.F. Theoretical considerations on the role of membrane potential in the regulation of endosomal pH. Biophys. J. 73:1997;674-687.
10. +-ATPase in regulating ATP-dependent endosome acidification. Proc. Natl Acad. Sci. USA. 86:1989;539-543.
11. + pump in parallel to a chloride conductance. J. Cell Biol. 97:1983;1303-1308.
12. Barasch J., Gershon M.D., Nunez E.A., Tamir H., Al-Awqati Q. Thyrotropin induces the acidification of the secretory granules of parafollicular cells by increasing the chloride conductance of the granular membrane. J. Cell Biol. 107:1988;2137-2147.
13. Demaurex N., Furuya W., D'Souza S., Bonifacino J.S., Grinstein S. Mechanism of acidification of the trans-Golgi network (TGN). In situ measurements of pH using retrieval of TGN38 and furin from the cell surface. J. Biol. Chem. 273:1998;2044-2051.
14. Farinas J., Verkman A.S. Receptor-mediated targeting of fluorescent probes in living cells. J. Biol. Chem. 274:1999;7603-7606.
15. Kneen M., Farinas J., Li Y., Verkman A.S. Green fluorescent protein as a noninvasive intracellular pH indicator. Biophys. J. 74:1998;1591-1599.
16. Lippincott-Schwartz J., Yuan L.C., Bonifacino J.S., Klausner R.D. Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: evidence for membrane cycling from Golgi to ER. Cell. 56:1989;801-813.
17. Wong S.H., Low S.H., Hong W. The 17-residue transmembrane domain of beta-galactoside alpha 2,6-sialyltransferase is sufficient for Golgi retention. J. Cell Biol. 117:1992;245-258.
18. + exchanger in renal microvillus membrane vesicles. Nature. 299:1982;161-163.
19. + exchanger in renal microvillus membrane vesicles. J. Biol. Chem. 258:1983;6767-6771.
20. + exchanger. J. Membr. Biol. 90:1986;1-12.
21. Sano T., Cantor C.R. Expression of a cloned streptavidin gene in Escherichia coli. Proc. Natl Acad. Sci. USA. 87:1990;142-146.
22. +-ATPase in living cells. Proc. Natl Acad. Sci. USA. 86:1989;544-548.
23. Hofer A.M., Schlue W.R., Curci S., Machen T.E. Spatial distribution and quantitation of free luminal [Ca] within the InsP3-sensitive internal store of individual BHK-21 cells: ion dependence of InsP3-induced Ca release and reloading. FASEB J. 9:1995;788-798.
24. Garcia A.M., Miller C. Channel-mediated monovalent cation fluxes in isolated sarcoplasmic reticulum vesicles. J. Gen. Physiol. 83:1984;819-839.
25. Wachter R., Remington J. Sensitivity of the yellow variant of green fluorescent protein to halides and nitrate. Curr. Biol. 9:1999;628-629.
26. McNeil P.L., Murphy R.F., Lanni F., Taylor D.L. A method for incorporating macromolecules into adherent cells. J. Cell Biol. 98:1984;1556-1564.
27. Teter K., Chandy G., Quiñones B., Pereyra K., Machen T., Moore H.P. Cellubrevin-targeted fluorescence uncovers heterogeneity in the recycling endosomes. J. Biol. Chem. 273:1998;19625-19633.