Methylmercury has a selective effect on mitochondria in cultured astrocytes in the presence of [U-13C]glutamate

Brain Research

Volumn 908, Issue 2, 2001, Pages 149-154

  Allen, Jeffrey W ^a   El Oqayli, Haytham ^b   Aschner, Michael ^a   Syversen, Tore ^b   Sonnewald, Ursula ^b  

^a WAKE FOREST SCHOOL OF MEDICINE   (United States)

^b NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

Author keywords

Astrocytes; Glutamate; Methylmercury; Mitochondria; MR spectroscopy

Indexed keywords

2 OXOGLUTARIC ACID; ASPARTIC ACID; GLUCOSE; GLUTAMIC ACID; GLUTAMINE; GLUTATHIONE; LACTIC ACID; MALIC ACID; METHYLMERCURY; OXALOACETIC ACID;

AMINO ACID SYNTHESIS; AMINO ACID TRANSPORT; ANIMAL TISSUE; ARTICLE; ASTROCYTE; CARBON NUCLEAR MAGNETIC RESONANCE; CELL COMPARTMENTALIZATION; CITRIC ACID CYCLE; CONTROLLED STUDY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLITE; MITOCHONDRIAL RESPIRATION; NEWBORN; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; ANIMALS, NEWBORN; ASPARTIC ACID; ASTROCYTES; CARBON RADIOISOTOPES; CELLS, CULTURED; CEREBRAL CORTEX; CITRIC ACID CYCLE; ENERGY METABOLISM; GLUTAMIC ACID; GLUTAMINE; GLUTATHIONE; MERCURY POISONING, NERVOUS SYSTEM; METHYLMERCURY COMPOUNDS; MITOCHONDRIA; RATS; RATS, SPRAGUE-DAWLEY;

EID: 0035958569     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-8993(01)02628-2     Document Type: Article

References (43)

1. Reactive oxygen species formation as a biomarker of methylmercury and trimethyltin neurotoxicity
2. Isolation of neonatal rat cortical astrocytes for primary cultures
3. Methylmercury-induced alterations in excitatory amino acid transport in rat primary astrocyte cultures
4. Nonvesicular release of neurotransmitter
5. In vitro evidence for the role of glutamate in the CNS toxicity of mercury
6. Increases in the number of reactive glia in the visual cortex of Macaca fascicularis following subclinical long-term methyl mercury exposure
7. Changes in the number of astrocytes and microglia in the thalamus of the monkey Macaca fascicularis following long-term subclinical methylmercury exposure
8. The effects of glutathione glycoside in methyl mercury poisoning
9. Astrocytes as mediators of methylmercury neurotoxicity: Effects on D-aspartate and serotonin uptake
10. Effects of prenatal and postnatal methylmercury exposure from fish consumption on neurodevelopment: Outcomes at 66 months of age in the Seychelles Child Development Study
11. Dissociation of neonatal rat brain by dispase for preparation of primary astrocyte cultures
12. Changes in the amino acid content of nerve endings (synaptosomes) induced by drugs that alter the metabolism of glutamate and γ-aminobutyric acid
13. Cellular distribution and kinetic properties of high-affinity glutamate transporters
14. Cognitive deficit in 7-year-old children with prenatal exposure to methylmercury
15. Astrocytes: Glutamate producers for neurons
16. 3H]D-aspartate from GABAergic neurons caused by reversal of glutamate transporters
17. Primary structure and functional characterization of a high-affinity glutamate transporter
18. Methylmercury-induced neurotoxicity in cerebral neuron culture is blocked by antioxidants and NMDA receptor antagonists
19. Energy on demand
20. Ultrastructural evidence that mercuric chloride lowers the threshold for glutamate neurotoxicity in an organotypic culture of rat cerebellum
21. Exogenous glutamate concentration regulates the metabolic fate of glutamate in astrocytes
22. The role of -SH groups in methylmercuric-chloride-induced D-aspartate and rubidium release from rat primary astrocyte cultures
23. Dynamic changes in glucose metabolism by lactate loading as revealed by a positron autoradiography technique using rat living brain slices
24. Effects of prenatal methylmercury exposure from a high fish diet on developmental milestones in the Seychelles Child Development Study
25. High extracellular potassium concentrations stimulate oxidative metabolism in a glutamatergic neuronal culture and glycolysis in cultured astrocytes but have no stimulatory effect in a GABAergic neuronal culture
26. Cloning and expression of a rat brain L-glutamate transporter
27. Localization of neuronal and glial glutamate transporters
28. Bcl-2 expression decreases methyl mercury-induced free-radical generation and cell killing in a neural cell line
29. Cellular resistance to methylmercury
30. A developmental study of lactate dehydrogenase isozyme and aspartate aminotransferase activity in organotypic rat hippocampal slice cultures and primary cultures of mouse neocortical and cerebellar neurons
31. Brain lactate is an obligatory aerobic energy substrate for functional recovery after hypoxia: Further in vitro validation
32. Lactate-supported synaptic function in the rat hippocampal slice preparation
33. Increased vulnerability of neurones and glial cells to low concentrations of methylmercury in a prooxidant situation
34. Mitochondrial heterogeneity in the brain at the cellular level
35. MRS study of glutamate metabolism in cultured neurons/glia
36. 13C NMR spectroscopy: Incorporation of more label into lactate than into glutamine demonstrates the importance of the TCA cycle
37. Structure, expression, and functional analysis of a Na(+)-dependent glutamate/aspartate transporter from rat brain
38. Triggering and execution of neuronal death in brain ischaemia: Two phases of glutamate release by different mechanisms
39. Effect of ischaemia and reperfusion on the extra- and intracellular distribution of glutamate, glutamine, aspartate and GABA in the rat hippocampus, with a note on the effect of the sodium channel blocker
40. Mitochondrial compartmentation at the cellular level: Astrocytes and neurons
41. 13C nuclear magnetic resonance spectroscopy
42. Anaerobic glycolysis is crucial for the maintenance of neuronal activity in guinea pig hippocampal slices
43. Role of nitric oxide in the cerebellar degeneration during methylmercury intoxication