MRS study of glutamate metabolism in cultured neurons/glia

Neurochemical Research

Volumn 21, Issue 9, 1996, Pages 987-993

  Sonnewald, Ursula ^a   White, Linda R ^b   Ødegård, Elin ^b   Westergaard, Niels ^c   Bakken, Inger J ^a,b   Aasly, Jan ^b   Unsgård, Geirmund ^b   Schousboe, Arne ^c  

^a SINTEF   (Norway)

^b TRONDHEIM UNIVERSITY HOSPITAL   (Norway)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

3 nitropropionic acid; 13C NMR spectroscopy; U 13C glutamate astrocytes; aminooxyacetic acid; neurons

Indexed keywords

4 AMINOBUTYRIC ACID RECEPTOR; AMINOOXYACETIC ACID; GLUTAMIC ACID; LACTIC ACID;

AMINO ACID METABOLISM; ANIMAL TISSUE; ARTICLE; BRAIN METABOLISM; CELL CULTURE; CITRIC ACID CYCLE; MOUSE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; TRANSAMINATION;

ANIMALS; ASPARTIC ACID; CARBON RADIOISOTOPES; CELLS, CULTURED; CEREBELLUM; CITRIC ACID CYCLE; EMBRYO; GAMMA-AMINOBUTYRIC ACID; GLUTAMIC ACID; LACTATES; MAGNETIC RESONANCE SPECTROSCOPY; MICE; NEUROGLIA; NEURONS; PREFRONTAL CORTEX; RADIOISOTOPE DILUTION TECHNIQUE; SUCCINATES;

ANIMALIA;

EID: 0029799731     PISSN: 03643190     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02532408     Document Type: Article

References (41)

1. 31P nuclear magnetic resonance studies on superfused cerebral tissue. Biochem. Soc. Trans. 13:835-839.
2. + under hypoxic and hypoglycaemic conditions in vitro. J. Neurochem. 51:172-176.
3. 31P-n.m.r. study. Biochem. J. 255:523-527.
4. Bachelard, H. S., K. J. Brooks, and O. Garofalo. 1991. Studies on the compartmentation of DOG metabolism in the brain. Neurochem. Res. 16:1025-1030.
5. 13C-n.m.r. spectroscopy. Biochem. J. 266:133-139.
6. 13C-n.m.r. study. Biochem. J. 282:225-230.
7. 19F NMR study. J. Neurochem. 61:2207-2214.
8. Schousboe, A., E. Meier, J. Drejer, and L. Hertz. 1989. Preparation of primary cultures of mouse (rat) cerebellar granule cells. In A Dissection and Tissue Culture Manual for The Central Nervous System. A. Shahar, J. de-Vellis, A. Vernadakis, and B. Haber, editors. Alan R. Liss, New York. 203-206.
9. Hertz, E., A. C. H. Yu, L. Hertz, B. H. J. Juurlink, and A. Schousboe. 1989. Preparation of primary cultures of mouse cortical neurons. In A Dissection and Tissue Culture Manual for The Central Nervous System. A. Shahar, J. de-Vellis, A. Vernadakis, and B. Haber, editors. Alan R. Liss, New York, 183-186
10. Hertz, L., B. H. J. Juurlink, E. Hertz, H. Fosmark, and A. Schousboe. 1989. Preparation of primary cultures of mouse (rat) astrocytes. In A Dissection and Tissue Culture Manual for The Central Nervous System. A. Shahar, J. De Vellis, A. Vernadakis, and B. Haber, editors. Alan R. Liss, New York. 105-108.
11. Lowry, O. H., N. J. Rosenbrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:265-275.
12. 13C]glutamate in astrocytes studied by 13C NMR spectroscopy: Incorporation of more label into 1actate than into glutamine demonstrates the importance of the TCA cycle. J. Neurochem. 61:1179-1182.
13. 13C]glutamate: implications for neurodegenerative diseases. Glia in press.
14. Wullner, U., A. B. Young, J. B. Penney, and M. F. Beal. 1994. 3-Nitropropionic acid toxicity in the striatum. J. Neurochem. 63: 1772-1781.
15. Brouillet, E. and M. F. Beal. 1993. NMDA antagonists partially protect against MPTP induced neurotoxicity in mice. Neuroreport. 4:387-390.
16. Brouillet, E., B. G. Jenkins, B. T. Hyman, R. J. Ferrante, N. W. Kowall, R. Srivastava, D. S. Roy, B. R. Rosen, and M. F. Beal. 1993. Age-dependent vulnerability of the striatum to the mitochondrial toxin 3-nitropropionic acid. J. Neurochem. 60:356-359.
17. Siesjö, B. K. and F. Bengtsson. 1989. Calcium fluxes, calcium antagonists, and calcium-related pathology in brain ischemia, hypoglycemia, and spreading depression: a unifying hypothesis. J. Cereb. Blood Flow and Metab. 9:127-140.
18. Beal, M. F., B. T. Hyman, and W. Koroshetz. 1993. Do defects in mitochondrial energy metabolism underlie the pathology of neurodegenerative diseases? Trends. Neurosci. 16:125-131.
19. Urbanska, E., C. Ikonomidou, M. Sieklucka, and W. A. Turski. 1991. Aminooxyacetic acid produces excitotoxic lesions in rat striatum. Synapse 9:129-135.
20. Beal, M. F., K. J. Swartz, B. T. Hyman, E. Storey, S. F. Finn, and W. Koroshetz. 1991. Aminooxyacetic acid results in excitotoxin lesions by a novel indirect mechanism. J. Neurochem. 57: 1068-1073.
21. Hertz, L., P. H. Wu, and A. Schousboe. 1978. Evidence for net uptake of GABA into mouse astrocytes in primary cultures - its sodium dependence and potassium independence. Neurochem. Res 3:313-323.
22. Schousboe, A., G. Svenneby, and L. Hertz. 1977. Uptake and metabolism of glutamate in astrocytes cultured from dissociated mouse brain hemispheres. J. Neurochem. 29:999-1005.
23. Hertz, L. 1979. Functional interactions between neurons and astrocytes I. Turnover and metabolism of putative amino acid transmitters. Prog. Neurobiol. 13:277-323.
24. Shank, R. P. and M. H. Aprison. 1977. Glutamine uptake and metabolism by the isolated toad brain: evidence pertaining to its proposed role as a transmitter precursor. J. Neurochem. 28:1189-1196.
25. Farinelli, S. E. and W. J. Nicklas. 1992. Glutamate metabolism in rat cortical astrocyte cultures. J. Neurochem. 58:1905-1915.
26. Waniewski, R. A. and D. L. Martin. 1986. Exogenous glutamate is metabolized to glutamine and exported by rat primary astrocyte cultures. J. Neurochem. 47:304-313.
27. Yu, A. C., T. E. Fisher, E. Hertz, J. T. Tildon, A. Schousboe, and L. Hertz. 1984. Metabolic fate of [14C]-glutamine in mouse cerebral neurons in primary cultures. J. Neurosci. Res 11:351-357.
28. 15N]glutamate by cultured astrocytes. Biochem. J. 234:185-192.
29. Zielke, H. R., J. T. Tildon, M. E. Landry, and S. R. Max. 1990. Effect of 8-bromo-cAMP and dexamethasone on glutamate metabolism in rat astrocytes. Neurochem. Res 15:1115-1122.
30. McKenna, M. C., U. Sonnewald, X. Huang, J. Stevenson, and R. H. Zielke. 1996. Exogenous glutamate concentration regulates the metabolic fate of glutamate in astrocytes. J. Neurochem. 66:386-393.
31. Cooper, A. J. and F. Plum. 1987. Biochemistry and physiology of brain ammonia. Physiol. Rev. 67:440-519.
32. Plaitakis, A. and S. Berl. 1988. Pathology of glutamate dehydrogenase. In Glutamine and glutamate in mammals. E. Kvamme, editor. CRC Press, Boca Raton, Fl. 128-140.
33. Erecinska, M. and D. Nelson. 1994. Effects of 3-nitropropionic acid on synaptosomal energy and transmitter metabolism: relevance to neurodegenerative brain diseases. J. Neurochem. 63: 1033-1041.
34. Hassel, B. and U. Sonnewald. 1995. Selective inhibition of the TCA cycle of GABAergic neurons with 3-nitropropionic acid in vivo. J. Neurochem. in press:
35. Leo, G. C., B. F. Driscoll, R. P. Shank, and E. Kaufman. 1993. Analysis of [1-13C]D-glucose metabolism in cultured astrocytes and neurons using nuclear magnetic resonance spectroscopy. Dev. Neurosci. 15:282-288.
36. 13C glucose metabolism in neocortical astrocytes: Evidence for mitochondrial heterogeneity. Dev. Neurosci. 15:351-358.
37. Hassel, B., U. Sonnewald, G. Unsgard, and F. Fonnum. 1994. NMR spectroscopy of cultured astrocytes: effects of glutamine and the gliotoxin fluorocitrate. J. Neurochem. 62:2187-2194.
38. Hassel, B. and U. Sonnewald. 1995. Glial formation of pyruvate and lactate from TCA cycle intermediates. J. Neurochem. in press:
39. Sonnewald, U., T. B. Müller, N. Westergaard, J. S. Svendsen, G. Unsgård, and A. Schousboe. 1994. Neuronal-glial interactions in glutamate and GABA homeostasis: Effects of hypoxia. In Pharmacology of Cerebral Ischemia. J. Krieglstein and H. Oberpichler-Schwenk, editors. Wissenschaftliche Verlagsgesellschaft, Stuttgart. 171-190.
40. 13C NMR spectroscopy may indicate compartmentation and mitochondrial heterogeneity. Neurosci. Lett. 185:24-28.
41. McKenna, M. C., N. Tonder, J. H. Stevenson, X. Huang, and R. Zielke. 1995. Synaptic terminals have higher mitochondrial malic enzyme activity than cortical or cerebellar neurons. J. Neurochem. 64:S90B Abstract.