Role of conserved nucleotides in building the 16 S rRNA binding site for ribosomal protein S15

Journal of Molecular Biology

Volumn 305, Issue 4, 2001, Pages 785-803

  Serganov, Alexander ^a,c   Bénard, Lionel ^b   Portier, Claude ^b   Ennifar, Eric ^a   Garber, Maria ^c   Ehresmann, Bernard ^a   Ehresmann, Chantal ^a  

^a INST DE BIOL MOLEC ET CELLULAIRE   (France)

^b INST DE BIOLOGIE PHYSICO CHIMIQUE   (France)

^c INSTITUTE OF PROTEIN RESEARCH   (Russian Federation)

Author keywords

Phylogenetic conservation; Ribosomal protein S15; RNA protein interaction; rRNA; Structure

Indexed keywords

PROTEIN S6; RIBOSOME PROTEIN; RIBOSOME PROTEIN S15; RIBOSOME PROTEIN S18; RNA 16S; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; CRYSTALLOGRAPHY; ESCHERICHIA COLI; MOLECULAR MODEL; NONHUMAN; PHYLOGENY; PRIORITY JOURNAL; PROTEIN RNA BINDING; SITE DIRECTED MUTAGENESIS; THERMUS THERMOPHILUS;

ESCHERICHIA COLI; THERMUS THERMOPHILUS;

EID: 0035951286     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4354     Document Type: Article

References (56)

1. A hierarchy of RNA subdomains in assembly of the central domain of the 30 S ribosomal subunit
2. Structure of the S15,S6,S16,S18-rRNA complex: Assembly of the 30 S ribosome central domain
3. Placement of protein and RNA structures into a 5 Å-resolution map of the 50 S ribosomal subunit
4. The complete atomic structure of the large ribosomal subunit at 2.4 Å resolution
5. Interaction of the Bacillus stearothermophilus ribosomal protein S15 with 16 S rRNA. I. Defining the minimal RNA site
6. Interaction of the Bacillus stearothermophilus ribosomal protein S15 with 16 S rRNA. II. Specificity determinants of RNA-protein recognition
7. Effects of polyvalent cations on the folding of an rRNA three-way junction and binding of ribosomal protein S15
8. Identification in a pseudoknot of a U.G motif essential for the regulation of the expression of ribosomal protein S15
9. Solution structure of the ribosomal RNA binding protein S15 from Thermus thermophilus
10. Crystallography and NMR system: A new software suited for macromolecular structure determination
11. A recurrent RNA-binding motif is appended to eucaryotic aminoacyl-tRNA synthetases
12. X-ray crystal structures of 70 S ribosome functional complexes
13. Conformational variability of the N-terminal helix in the stucture of ribosomal protein S15
14. Structure of bacterial 30 S ribosomal subunit at 5.5 Å resolution
15. Crystal structure of a conserved ribosomal protein-RNA complex
16. Identification of an RNA-protein bridge spanning the ribosomal sub-unit interface
17. The aminoacyl-tRNA synthetases family: Modules at work
18. Themes in RNA-protein recognition
19. A pseudoknot is required for efficient translational initiation and regulation of the Escherichia coli rpsO gene coding for ribosomal protein S15
20. Interactions of ribosomal proteins S6, S8; S15 and S18 with the central domain of 16 S rRNA from Escherichia coli
21. Lessons from an evolving rRNA: 16 S and 23 S rRNA structures from a comparative perspective
22. Assembly mapping of 30 S ribosomal proteins from Escherichia coli. Further studies
23. Anchoring an extended HTLV-1 Rex peptide within an RNA major groove containing junctional base triples
24. Improved methods for the building of protein models in electron density maps and the location of errors in these models
25. Rapid and efficient site-specific mutagenesis without phenotypic selection
26. In vivo determination of RNA structure-function relationship: Analysis of the 790 loop in ribosomal RNA
27. lac repressor binding to non-operator DNA: Detailed studies and comparison of equilibrium and rate competition methods
28. Characterizations of the solution conformations of unbound and Tat peptide-bound forms of HIV-1 TAR RNA
29. Structure of Escherichia coli ribosomal protein L25 complexed with a 5 S rRNA fragment at 1.8 Å resolution
30. A novel loop-loop recognition motif in the yeast ribosomal protein L30 autoregulation RNA complex
31. Nucleotides in 16 S rRNA protected by the association of 30 S and 50 S ribosomal subunits
32. Binding of tRNA to the ribosomal A and P sites protects two distinct sets of nucleotides in 16 S rRNA
33. The E. coli 16 S rRNA binding site of ribosomal protein S15: Higher-order structure in the absence and in the presence of the protein
34. Crystal structure of the S15-rRNA complex
35. The structural basis of ribosome activity in peptide bond synthesis
36. On the origin of the ribosome: Coevolution of subdomains of tRNA and rRNA
37. 2+-induced conformational changes in the S15 binding site of 16 S ribosomal RNA
38. Crystal structure at 1.82 Å resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin
39. Target site of Escherichia coli ribosomal protein S15 on its messenger RNA. Conformation and interaction with the protein
40. Ribosomal protein S15 from Escherichia coli modulates its own translation by trapping the ribosome on the mRNA initiation loading site
41. Molecular dissection of the pseudoknot governing the translational regulation of Escherichia coli ribosomal protein S15
42. Translational autocontrol of the Escherichia coli ribosomal protein S15
43. Hydroxyl radical footprinting of ribosomal proteins on 16 S rRNA
44. Crystal structure of the spliceosomal U2B″-U2A′ protein complex bound to a fragment of U2 small nuclear RNA
45. Conformation of the TAR RNA-arginine complex by NMR spectroscopy
46. Structure of functionally activated small ribosomal subunit at 3.3 Å resolution
47. The 16 S rRNA binding site of Thermus thermophilus ribosomal protein S15: Comparison with Escherichia coli S15, minimum site and structure
48. Ribosomal protein S15 from Thermus thermophilus: Cloning, sequencing, overexpression of the gene and RNA-binding properties of the protein
49. The NMR structure of the 5 S rRNA E-domain-protein L25 complex shows preformed and induced recognition
50. Use of T7 polymerase to direct expression of cloned genes
51. Interaction of ribosomal proteins, S6, S8, S15 and S18 with the central domain of 16 S ribosomal RNA
52. The small ribosomal subunit from Thermus thermophilus at 4.5 Å resolution: Pattern fittings and the identification of a functional site
53. Deciphering RNA recognition: Aminoglycoside binding to the hammerhead ribozyme
54. An investigation of the 16 S RNA binding sites of ribosomal proteins S4, S8, S15, and S20 from Escherichia coli
55. A detailed view of a ribosomal active site: The structure of the L11-RNA complex
56. Location and characteristics of ribosomal protein binding sites in the 16 S RNA of Escherichia coli