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Volumn 288, Issue 5463, 2000, Pages 107-112

Structure of the S15,S60,S18-rRNA complex: Assembly of the 30S ribosome central domain

Author keywords

[No Author keywords available]

Indexed keywords

RIBONUCLEOPROTEIN; RIBOSOME RNA; BACTERIAL PROTEIN; BACTERIAL RNA; PROTEIN S6; RIBOSOMAL PROTEIN S15; RIBOSOMAL PROTEIN S18; RIBOSOME PROTEIN;

EID: 0034615759     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.288.5463.107     Document Type: Article
Times cited : (186)

References (36)
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    • N. Ban et al., Nature 400, 841 (1999).
    • (1999) Nature , vol.400 , pp. 841
    • Ban, N.1
  • 10
    • 0029909551 scopus 로고    scopus 로고
    • A. A. Serganov et al., RNA 2, 1124 (1996).
    • (1996) RNA , vol.2 , pp. 1124
    • Serganov, A.A.1
  • 16
    • 0343682164 scopus 로고    scopus 로고
    • note
    • The root means square deviation (rmsd) for 182 common CA atoms and 71 common P atoms from the 5.5 Å 30S structure [PDB entry 1GD7 (1)] and the 7th T4 RNP is 2.7 Å. The rmsd for the phosphates in the two copies of the T4 RNA in the asymmetric unit was 1.33 Å. Weak electron density was observed above helix 22 in the Tth T4 RNA that is consistent with the coaxially stacked arrangement of this helix in the 30S model, although we were not able to build an atomic model for this region.
  • 21
    • 0343246503 scopus 로고    scopus 로고
    • note
    • rmsd values for the Cα atoms (Å): S15a versus S15b = 1.09, S15a versus PDB entry 1A32 (20) = 0.93, S15a versus PDB entry 1AB3 (19) = 3.26, where a and b refer to the two copies of S15 in the asymmetric unit.
  • 22
    • 0024278336 scopus 로고
    • M. S. Capel, M. Kjeldgaard, D. M. Engelman, P. B. Moore, J. Mol. Biol. 200, 65 (1988). Distances between protein centers of mass in the Tth T4 RNP and in the 30S subunit as measured by neutron scattering, in parentheses: S6a-S15a = 42 Å (70 ± 5), S6a ± S18a = 22 Å (33 ± 3), S15a-S18a = 45 Å (70 ± 5). The center of mass of the Tth S18 protein was calculated without 34 COOH-terminal residues that are disordered in the structure.
    • (1988) J. Mol. Biol. , vol.200 , pp. 65
    • Capel, M.S.1    Kjeldgaard, M.2    Engelman, D.M.3    Moore, P.B.4
  • 24
    • 0028203301 scopus 로고
    • M. Lindahl et al., EMBOJ. 13, 1249 (1994) (PDB entry 1RIS); D. E. Otzen, O. Kristensen, M. Proctor, M. Oliveberg, Biochemistry 38, 6499 (1999) (PDB entry 1LOU). rmsd values for the Cα atoms (Å): S6a-S6b = 0.46, S6a-IRIS = 0.89, S6a-1LOU = 1.89, S18a-S18b = 1.13, where 3 and b refer to the two copies of the asymmetric unit.
    • (1994) EMBOJ. , vol.13 , pp. 1249
    • Lindahl, M.1
  • 25
    • 0033580679 scopus 로고    scopus 로고
    • M. Lindahl et al., EMBOJ. 13, 1249 (1994) (PDB entry 1RIS); D. E. Otzen, O. Kristensen, M. Proctor, M. Oliveberg, Biochemistry 38, 6499 (1999) (PDB entry 1LOU). rmsd values for the Cα atoms (Å): S6a-S6b = 0.46, S6a-IRIS = 0.89, S6a-1LOU = 1.89, S18a-S18b = 1.13, where 3 and b refer to the two copies of the asymmetric unit.
    • (1999) Biochemistry , vol.38 , pp. 6499
    • Otzen, D.E.1    Kristensen, O.2    Proctor, M.3    Oliveberg, M.4
  • 36
    • 0342811868 scopus 로고    scopus 로고
    • note
    • Supported by NIH grant GM53757 (J.R.W.). The coordinates of the Tth T4 RNP complex have been deposited in the Protein Data Bank (accession number IEKC). We thank D. Shcherbakov for providing the T. thermophilus S18 sequence in advance of publication. We thank G. Joyce, R. T. Batey, J. D. Puglisi, and J. Dinsmore for critical review of the manuscript, and the staff at Stanford Synchrotron Radiation Laboratory for their assistance.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.