Solution structure of Grb2 reveals extensive flexibility necessary for target recognition

Journal of Molecular Biology

Volumn 306, Issue 3, 2001, Pages 527-537

  Yuzawa, Satoru ^a,b,c   Yokochi, Masashi ^a,b   Hatanaka, Hideki ^d   Ogura, Kenji ^a,b   Kataoka, Mikio ^e   Miura, Kin Ichiro ^c   Mandiyan, Valsan ^f   Schlessinger, Joseph ^f   Inagaki, Fuyuhiko ^a,b  

^a HOKKAIDO UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^c GAKUSHUIN UNIVERSITY   (Japan)

^d TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE   (Japan)

^e NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

^f NEW YORK UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Flexibility; Grb2; NMR; SAXA; Solution structure

Indexed keywords

ADAPTOR PROTEIN; GROWTH FACTOR RECEPTOR BOUND PROTEIN 2; MEMBRANE RECEPTOR; MITOGEN ACTIVATED PROTEIN KINASE; RAS PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CRYSTAL STRUCTURE; HUMAN; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN STRUCTURE; PROTEIN TARGETING; RADIATION SCATTERING; SIGNAL TRANSDUCTION;

EID: 0035936696     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4396     Document Type: Article

References (46)

1. Membrane-targeting of signalling molecules by SH2/SH3 domain-containing adaptor proteins
2. Biased combinatorial libraries: Novel ligands for the SH3 domain of phosphatidylinositol 3-kinase
3. The Grb2-mSos1 complex binds phosphopeptides with higher affinity than Grb2
4. Enhanced affinities and specificities of consolidated ligands for the Src Homology (SH) 3 and SH2 domains of Abelson protein-tyrosine kinase
5. Binding of the Grb2 SH2 domain to phosphotyrosine motifs does not change the affinity of its SH3 domains for Sos proline-rich motifs
6. A Sos-derived peptidimer blocks the Ras signaling pathway by binding both Grb2 SH3 domains and displays antiproliferative activity
7. Fluorescence methods for studying equilibrium macromolecule-ligand interactions
8. 15N NMR relaxation
9. Direct determination of changes of interdomain orientation on ligation: Use of the orientational dependence of (15)N NMR relaxation in abl SH(32)
10. The solution structure of Abl SH3, and its relationship to SH2 in the SH(32) construct
11. Signaling-2000 and beyond
12. Surface, subunit interfaces and interior of oligomeric proteins
13. Melittin binding causes a large calcium-dependent conformational change in calmodulin
14. Structural characterization of the molten globule and native states of apomyoglobin by solution X-ray scattering
15. 15N inverse detected heteronuclear NMR spectroscopy: Application to Staphylococcal nuclease
16. Solution structure and ligand-binding site of the carboxy-terminal SH3 domain of Grb2
17. A lipid-anchored Grb2-binding protein that links FGF-Receptor activation to the Ras/MAPK signaling pathway
18. Modular peptide recognition domains in eukaryotic signaling
19. Independent binding of peptide ligands to the SH2 and SH3 domains of Grb2
20. Structural determinants of peptide-binding orientation and of sequence specificity in SH3 domains
21. Model-Free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. 1. Theory and range of validity
22. Model-Free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. 2. Analysis of experimental results
23. The SH2 and SH3 domain-containing protein Grb2 links receptor tyrosine kinases to ras signaling
24. Crystal structure of the mammalian Grb2 adaptor
25. A sensitive HN(CA)CO experiment for deuterated proteins
26. Cloning of ASH, a ubiquitous protein composed of one Src homology region (SH) 2 and two SH3 domains, from human and rat cDNA libraries
27. Small-Angle scattering. Information content and error analysis
28. Solution structure of the SH2 domain of Grb2 complexed with the Shc-derived phosphotyrosine-containing peptide
29. Comparative protein modelling by satisfaction of spatial restraints
30. How receptor tyrosine kinases activate Ras
31. SH2/SH3 signaling proteins
32. Crystal structure of the Src family tyrosine kinase Hck
33. The SH2/SH3 domain-containing protein GRB2 interacts with tyrosine-phosphorylated IRS1 and Shc: Implications for insulin control of ras signaling
34. Cyclosporin A-cyclophilin complex formation a model based on X-ray and NMR data
35. Small-angle-scattering-data treatment by the regularization method
36. Structure of the N-terminal SH3 domain of GRB2 complexed with a peptide from the guanine nucleotide releasing factor Sos
37. Aggregation of bovine serum albumin upon cleavage of its disulfide bonds, studied by time-resolved small-angle X-ray scattering technique with synchrotron radiaion
38. 15N labeling of proteins for NMR strudies
39. Flexibility of interdomain contacts revealed by topological isomers of bivalent consolidated ligands to the dual Src homology domain SH(32) of Abelson
40. Three-dimensional structure of the tyrosine kinase c-Src
41. 2H labeled proteins with high sensitivity