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Volumn 278, Issue , 1997, Pages 221-257

Fluorescence methods for studying equilibrium macromolecule-ligand interactions

(1)  Eftink, Maurice R a  

a NONE

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM BINDING PROTEIN; HAPTEN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; TRYPTOPHAN DERIVATIVE;

EID: 0030956737     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(97)78013-3     Document Type: Article
Times cited : (239)

References (68)
  • 10
    • 85030299958 scopus 로고    scopus 로고
    • note
    • 2 is for site j = 2 in a multisite system. Again, subscripts are not given when they are obvious for the model, i.e., K is used for a single-site model or for a model in which all the binding sites are identical.
  • 11
    • 85030295139 scopus 로고    scopus 로고
    • note
    • i,M.
  • 12
    • 85030293348 scopus 로고    scopus 로고
    • note
    • Any background signal (fluorescence or scattered light) from the buffer should be subtracted, if it is significant. Likewise, if the ligand titrant contributes to the signal (when the fluorescence from the macromolecule is being observed), then these contributions should be subtracted. The observed fluorescence signal of the sample either can be measured at a single emission wavelength or can be integrated over part or all of the emission envelope. If measurements are made at one emission wavelength, then it should be realized that the enhancement factors may depend on the selected wavelength, if there is a red or blue shift in fluorescence on binding. In either case, it is usually not necessary to correct the signals for any wavelength dependence of the photomultiplier response, since only relative signals are needed for the purpose of studying ligand binding.
  • 13
    • 85030294675 scopus 로고    scopus 로고
    • note
    • MLi values refer to the molar fluorescence of each bound ligand.
  • 25
    • 0017494663 scopus 로고
    • B. Birdsall, R. W. King, M. R. Wheeler, C. A. Lewis, S. R. Goode, R. B. Dunlap, and G. C. K. Roberts, Anal. Biochem. 132, 353 (1983); J. F. Holland, R. E. Teets, P. M. Kelly, and A. Timnick, Anal. Chem. 49, 706 (1977).
    • (1977) Anal. Chem. , vol.49 , pp. 706
    • Holland, J.F.1    Teets, R.E.2    Kelly, P.M.3    Timnick, A.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.