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Volumn 286, Issue 5447, 1999, Pages 2151-2153

Three-dimensional structures of the TAF(II)-containing complexes TFIID and TFTC

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE II; TATA BINDING PROTEIN; TRANSCRIPTION FACTOR; DNA BINDING PROTEIN; TAF10 PROTEIN, HUMAN; TATA BINDING PROTEIN ASSOCIATED FACTOR; TRANSCRIPTION FACTOR II; TRANSCRIPTION FACTOR IID;

EID: 0032589574     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.286.5447.2151     Document Type: Article
Times cited : (103)

References (36)
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    • 2] containing 500 mM KCl and two times with IP buffer containing 100 mM KCl. After washing, bound proteins were eluted with a 1000-fold excess of the corresponding epitope peptide and analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The polypeptide composition of the isolated complexes were verified either on silver-stained SDS-PAGE gels or by protein immunoblots as described (1, 8).
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    • The 3D models were calculated from conical tilt series produced by recording the same field of molecules at 60° and 0° tilt. Molecular images were extracted from the untilted micrographs, aligned, and classified into homogeneous groups to identify subpopulations of similarly oriented particles. Tilt and in-plane rotation parameters yield the Euler angles for each tilted view, about 100 of which were merged by filtered back-projection to calculate the 3D molecular envelope.
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    • We thank Y. Lutz for antibody production, J. C. Dantonel and B. Bell for discussions and critical reading of the manuscript, P. Eberling for peptide synthesis, the cell culture group for HeLa cells, J. C. Homo for help with electron microscopes, and R. Buchert, J.-M. Lafontaine, and B. Boulay for illustrations. M.B. was supported by a fellowship from the Ministère de la Recherche et Technologie. This work was supported by grants from CNRS, INSERM, the Höpital Universitaire de Strasbourg, Fondation pour la Recherche Médicale, the Association pour la Recherche contre le Cancer, the Ligue National Contre le Cancer, and the Human Frontier Science Program.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.