Modification of the fatty acid composition of Escherichia coli by coexpression of a plant acyl-acyl carrier protein desaturase and ferredoxin

Journal of Bacteriology

Volumn 178, Issue 3, 1996, Pages 936-939

  Cahoon, Edgar B ^a   Mills, Lisa A ^a   Shanklin, John ^a  

^a BROOKHAVEN NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; DESATURASE; FERREDOXIN; MONOUNSATURATED FATTY ACID; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; LIPID COMPOSITION; NONHUMAN; PLANT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

ESCHERICHIA COLI;

EID: 0030064049     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.3.936-939.1996     Document Type: Article

References (27)

1. Bligh, E. G., and W. J. Dyer. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37:911-917.
2. Bloch, K. 1970 β-Hydroxydecanoyl thioester dehydrase, p. 441-464. In P. D. Boyer (ed.), The enzymes, vol. 5, 3rd ed. Academic Press, Inc., New York.
3. 6 palmitoyl-acyl carrier protein desaturase in Thunbergia alata endosperm. J. Biol. Chem. 269:27519-27526.
4. Cahoon, E. B., and J. B. Ohlrogge. 1994. Apparent role of phosphatidylcholine in the metabolism of petroselinic acid in developing Umbelliferae endosperm. Plant Physiol. 104:845-855.
5. Dörmann, P., T. A. Voelker, and J. B. Ohlrogge. 1995. Cloning and expression in Escherichia coli of a novel thioesterase from Arabidopsis thaliana specific for long-chain acyl-acyl carrier proteins. Arch. Biochem. Biophys 316:612-618.
6. Gerdes, K. 1988. The PARB (HOK/SOK) locus of plasmid R1: a general purpose plasmid stabilization system. Bio/Technology 6:1402-1405.
7. Guerra, M. I., J. B. Ohlrogge, and M. Frentzen. 1986. Activity of acyl carrier protein isoforms in reactions of plant fatty acid metabolism. Plant Physiol. 82:448-453
8. Jaworski, J. G., and P. K. Stumpf. 1974. Fat metabolism in higher plants. Properties of a soluble stearyl-acyl carrier protein desaturase from maturing Carthamus tinctonus. Arch. Biochem Biophys 162:158-165
9. Jones, A., H. M. Davies, and T. A. Voelker. 1995. Palmitoyl-acyl carrier protein (ACP) thioesterase and the evolutionary origin of plant acyl-ACP thioesterases. Plant Cell 7:359-371.
10. Kater, M. M., G. M. Koningstein, H. J. J. Nijkamp, and A. R. Stuitje. 1994. The use of a hybrid genetic system to study the functional relationship between prokaryotic and plant multi-enzyme fatty acid synthetase complexes. Plant Mol. Biol. 24:771-790.
11. Knoell, H.-E., and J. Knappe. 1974. Escherichia coli ferredoxin, an iron-sulfur protein of the adrenodoxin type. Eur. J. Biochem. 50:245-252.
12. Magnuson, K., S. Jackowski, C. O. Rock, and J. E. Cronan, Jr. 1993. Regulation of fatty acid biosynthesis in Escherichia coli Microbiol. Rev. 57:522-542.
13. McKeon, T., and P. K. Stumpf. 1981. Stearoyl-acyl carrier protein desaturase from safflower seeds. Methods Enzymol. 71:275-281.
14. Morris, L. J., D. M. Wharry, and E. M. Hammond. 1967. Chromatographic behaviour of isomeric long-chain aliphatic compounds. II. Argentation thin-layer chromatography of isomeric octadecenoates J. Chromatogr. 31:69-76.
15. Morrison, W. R., and L. M. Smith. 1964 Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron trifluoride methanol. J. Lipid Res 5:600-608.
16. Nagai, J., and K. Bloch. 1968. Enzymatic desaturation of stearyl acyl carrier protein. J. Biol. Chem. 243:4626-4633
17. Ohlrogge, J., and J. Browse. 1995. Lipid biosynthesis. Plant Cell 7:957-970.
18. Ohlrogge, J., L. Savage, J. Jaworski, T. Voelker, and D. Post Beittenmiller. 1995. Alteration of acyl-acyl carrier protein pools and acetyl-CoA carboxylase expression in Escherichia coli by a plant medium chain acyl-acyl carrier protein thioesterase. Arch. Biochem. Biophys. 317:185-190.
19. + oxidoreductase. Plant Mol. Biol 26:679-690.
20. Roughan, G., and I. Nishida. 1990. Concentrations of long-chain acyl-acyl carrier proteins during fatly acid synthesis by chloroplasts isolated from pea (Pisum sativum), safflower (Carthamus tinctoris), and amaranthus (Amaranthus lividus) leaves. Arch. Biochem. Biophys. 276:38-16
21. Shimakata, T., and P. Stumpf. 1982. Isolation and function of spinach leaf β-ketoacyl-[acyl-carrier-protein] synthases. Proc. Natl. Acad. Sci USA 79: 5808-5812.
22. Studier, F. W., A. H. Rosenberg, J. J. Dunn, and J. W. Dubendorff. 1990. Use of 17 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:60-89.
23. Ta, D. T., and L. E. Vickery. 1992. Cloning, sequencing, and overexpression of a [2Fe-2S] ferredoxin gene from Escherichia coli. J. Biol. Chem. 267: 11120-11125
24. Thompson, G. A., D. E. Scherer, S. Foxall-Van Aken, J. W. Kenny, H. L. Young, D. K. Shintani, J. C. Kridl, and V. C. Knauf. 1991. Primary structures of the precursor and mature forms of the stearoyl-acyl carrier protein desaturase from safflower embryos and requirement of ferredoxin for enzyme activity. Proc. Natl. Acad. Sci. USA 88:2578-2582.
25. Voelker, T. A., and H. M. Davies. 1994. Alteration of the specificity and regulation of fatty acid synthesis of Escherichia coli by expression of a plant medium-chain acyl-acyl carrier protein thioesterase J. Bacteriol 176:7320-7327.
26. Yamamoto, K., A. Shibahara, T. Nakayama, and G. Kajimoto. 1991 Determination of double-bond positions in methylene-interrupted dienoic fatty acids by GC-MS as their dimethyl disulfide adducts. Chem. Phys. Lipids 60:39-50
27. + oxidoreductase. Methods Enzymol 69:250-252.