Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: The pH behaviour of Trichoderma reesei Cel7A and its E223S/A224H/L225V/T226A/D262G mutant

Biochemical Journal

Volumn 356, Issue 1, 2001, Pages 19-30

  Becker, Dieter ^a   Braet, Christophe ^a   Brumer III, Harry ^a   Claeyssens, Marc ^a   Divne, Christina ^a   Fagerström, B Richard ^a   Harris, Mark ^a   Jones, T Alwyn ^a   Kleywegt, Gerard J ^a   Koivula, Anu ^a   Mahdi, Sabah ^a   Piens, Kathleen ^a   Sinnott, Michael L ^a   Ståhlberg, Jerry ^a   Teeri, Tuula T ^a   Underwood, Melanie ^a   Wohlfahrt, Gerd ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Cellulase; Cellulose; Endoglucanase; Enzyme kinetics; pH dependence

Indexed keywords

CRYSTAL STRUCTURE; ENZYMES; HYDROGEN BONDS; HYDROLYSIS; PH EFFECTS; SUBSTRATES; X RAY CRYSTALLOGRAPHY;

MUTANTS;

BIOCHEMISTRY;

AMINO ACID; CELLOBIOSE; CELLULASE; CELLULOSE; CELLULOSE 1,4 BETA CELLOBIOSIDASE; GLUCAN SYNTHASE; MUTANT PROTEIN;

ALKALINITY; ARTICLE; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME ENGINEERING; ENZYME KINETICS; HYDROGEN BOND; HYDROLYSIS; IONIZATION; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN FAMILY; TRICHODERMA REESEI; X RAY CRYSTALLOGRAPHY;

ALKALIES; CATALYTIC DOMAIN; CELLOBIOSE; CELLULASE; CELLULOSE; CELLULOSE 1,4-BETA-CELLOBIOSIDASE; CRYSTALLOGRAPHY, X-RAY; ENZYME STABILITY; HISTIDINE; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MUTATION; PROTEIN ENGINEERING; TRICHODERMA;

HUMICOLA; HUMICOLA INSOLENS; HYPOCREA JECORINA; TRICHODERMA;

EID: 0035874465     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3560019     Document Type: Article

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