Crystal structure of the family 7 endoglucanase I (Cel7B) from Humicola insolens at 2.2 Å resolution and identification of the catalytic nucleophile by trapping of the covalent glycosyl-enzyme intermediate

Biochemical Journal

Volumn 335, Issue 2, 1998, Pages 409-416

  Mackenzie, Lloyd F ^a   Sulzenbacher, Gerlind ^b   Divne, Christina ^c   Jones, T Alwyn ^c   Wöldike, Helle F ^d   Schülein, Martin ^d   Withers, Stephen G ^a   Davies, Gideon J ^b  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF YORK   (United Kingdom)

^c UPPSALA UNIVERSITY   (Sweden)

^d NOVO NORDISK A S   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

CELLULASE; CELLULOSE; FUNGAL PROTEIN; GLUCAN SYNTHASE; GLYCOSIDASE; POLYSACCHARIDE;

AMINO ACID SEQUENCE; ARTICLE; CELL WALL; CONTROLLED STUDY; CRYSTAL STRUCTURE; DEGLYCOSYLATION; DEGRADATION; ENZYME ACTIVE SITE; ENZYME INACTIVATION; ENZYME KINETICS; FUNGUS; HYDROLYSIS; MACROMOLECULE; MASS SPECTROMETRY; NONHUMAN; PLANT CELL; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STOICHIOMETRY;

AMIDOHYDROLASES; CATALYTIC DOMAIN; CELLULASE; CELLULOSE 1,4-BETA-CELLOBIOSIDASE; CRYSTALLOGRAPHY, X-RAY; ENZYME ACTIVATION; GLUCOSIDES; MASS SPECTROMETRY; MITOSPORIC FUNGI; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL) ASPARAGINE AMIDASE; PROTEIN CONFORMATION; SOLUTIONS;

EID: 0032532639     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/bj3350409     Document Type: Article

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