Immunoaffinity chromatographic purification of cellobiohydrolase II mutants from recombinant Trichoderma reesei strains devoid of major endoglucanase genes

Protein Expression and Purification

Volumn 8, Issue 4, 1996, Pages 391-400

  Koivula, Anu ^a   Lappalainen, Arja ^a   Virtanen, Susanna ^a   Mäntylä, Arja L ^b   Suominen, Pirkko ^b   Teeri, Tuula T ^a  

^a VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

^b Rohm Enzyme Finland Oy   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

HYPOCREA JECORINA; TRICHODERMA;

CELLULASE; CELLULOSE 1,4 BETA CELLOBIOSIDASE; FUNGAL PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING SITE; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETICS; METHODOLOGY; PEPTIDE MAPPING; RESTRICTION MAPPING; TRICHODERMA; WESTERN BLOTTING;

BINDING SITES; BLOTTING, WESTERN; CELLULASE; CELLULOSE 1,4-BETA-CELLOBIOSIDASE; CHROMATOGRAPHY, AFFINITY; ENZYME-LINKED IMMUNOSORBENT ASSAY; FUNGAL PROTEINS; PEPTIDE MAPPING; RESTRICTION MAPPING; TRICHODERMA;

EID: 0030462043     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0116     Document Type: Article

References (34)

1. 1. Nevalainen, H., and Penttilä, M. (1995) Molecular biology of cellulolytic fungi, in "The Mycota II. Genetics and Biotechnology" (Kück, Ed.), pp. 303-319, Springer-Verlag, Berlin/Heidelberg.
2. 2. Rouvinen, J., Bergfors, T., Teeri, T., Knowles, J. K. C., and Jones, T. A. 1990. Three-dimensional structure of cellobiohydrolase II from Trichoderma reesei. Science 249, 380-386.
3. 3. Divne, C., Ståhlberg, J., Reinikainen, T., Ruohonen, L., Pettersson, G., Knowles, J. K. C., Teeri, T. T., and Jones, A. (1994) The three-dimensional structure of cellobiohydrolase I from Trichoderma reesei reveals a lysozyme-like active site on a lectin-like framework. Science 265, 524-528.
4. 4. Reinikainen, T., Teleman, O., and Teeri, T. (1995) Effects of pH and high ionic strength on the adsorption and activity of native and mutated cellobiohydrolase I from Trichoderma reesei. Proteins Struct. Funct. Genet. 22, 392-403.
5. 5. Srisodsuk, M., Reinikainen, T., Penttilä, M., and Teeri, T. (1993) Role of the interdomain linker pep tide of trichoderma reesei cellobiohydrolase I in its interaction with crystalline cellulose. J. Biol. Chem. 268, 20756-20761.
6. 6. Reinikainen, T., Henriksson, K., Siika-aho Matti, Teleman, O., and Poutanen, K. (1995) Low-level endoglucanase contamination in a Trichoderma reesei cellobiohydrolase II preparation affects its enzymatic activity on β-glucan. Enzyme Microb. Technol. 17, 888-892.
7. 7. Haab, D., Hagspiel, K., Szakmary, K., and Kubicek, C. P. (1990) Formation of the extracellular proteases from Trichoderma reesei QM 9414 involved in cellulase degradation. J. Biotechnol. 16, 187-198.
8. 8. Sprey, B., and Lambert, C. (1983) Titration curves of cellulases from Trichoderma reesei: Demonstration of a cellulase-xylanase-b-glucosidase complex. FEMS Microbiol. Lett. 18, 217-222.
9. 9. Tomme, P., McRae, S., Wood, T. M., and Claeyssens, M. (1988) Chromatographic separation of cellulolytic enzymes. Methods Enzymol. 160, 187-192.
10. 10. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) "Molecular Cloning. A Laboratory Manual," 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
11. 11. Suominen, P. L., Mäntylä, A. L., Karhunen, T., Hakola, S., and Nevalainen, H. (1993) High frequency one-step gene replacement in Trichoderma reesei. II. Effect of deletions of individual genes. Mol. Gen. Genet. 241, 523-530.
12. 12. Bailey, M. J., and Nevalainen, H. (1981) Induction, isolation and testing of stable Trichoderma reesei mutants with improved production of solubilizing cellulase. Enzyme Microb. Technol. 3, 153-157.
13. 13. Suominen, P., Mäntylä, A., Saarelainen, R., Paloheimo, M., Fagerström, R., Parkkinen, E., and Nevalainen, H. (1992) Genetic engineering of Trichoderma reesei to produce suitable enzyme combinations for applications in the pulp and paper industry, in "Proceedings of the 5th International Conference on Biotechnology in the Pulp and Paper Industry" (Kuwahara, M., and Shimada, M., Eds.), pp. 439-445, Kyoto, Japan.
14. 14. Koivula, A., Reinikainen, T., Ruohonen, L., Valkeajärvi, A., Claeyssens, M., Teleman, O., Kleywegt, G., Szardenings, M., Rouvinen, J., Jones, T. A., and Teeri, T. T. 1996. The active site of Trichoderma reesei cellobiohydrolase II: The role of tyrosine-169. Protein Eng. 9, 691-699.
15. 15. Penttilä, M., Nevalainen, H., Rättö, M., Salminen, E., and Knowles, J. K. C. (1987). A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei. Gene 61, 155-164.
16. 16. Mattern, I. E., Punt, P. T., Unkles, S., Powels, P. H., and van den Hondel, C. A. M. J. J. (1987) "Abstract of the 19th Lunteren Lectures on Molecular Biology," p. 34, Lunteren, The Netherlands.
17. 17. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature 227, 680-685.
18. 18. Bühler, R. (1991) Double-antibody sandwich enzyme-linked immunosorbent assay for quantitation of endoglucanase I of Trichoderma reesei. Appl. Environ. Microbiol. 57, 3317-3321.
19. 19. Aho, S. Olkkonen, V., Jalava, T., Paloheimo, M., Bühler, R., Niku-Paavola, M.-L., Bamford, D. H., and Korhola, M. (1991) Monoclonal antibodies against core and cellulose-binding domains of Trichoderma reesei cellobiohydrolases I and II and endoglucanase I. Eur. J. Biochem. 200, 643-649.
20. 20. Stern, A. S., and Podlaski, F. J. (1993) Increasing the antigen binding capacity of immobilized antibodies, in "Techniques in Protein Chemistry IV," pp. 353-360, Academic Press, San Diego.
21. 21. Niku-Paavola, M.-L., Lappalainen, A., Enari, T.-M., and Nummi, M. (1986) Trichoderma reesei cellobiohydrolase II. Purification by immunoadsorption and hydrolytic properties. Biotechnol. Appl. Biochem. 8, 449-458.
22. 22. Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354.
23. 23. van Tilbeurgh, H., Loontiens, F. G., de Bruyne, C. K., and Claeyssens, M. (1988) Fluorogenic and chromogenic glycosides as substrates and ligands of carbohydrases. Methods Enzymol. 160, 46-59.
24. 24. Claeyssens, M. (1988) The use of chromophoric substrates and specific assays in the study of structure-activity relationships of cellulolytic enzymes, in, "Biochemistry and Genetics of Cellulose Degradation" (Aubert, J.-P., Beguin, P., and Millet, J., (Eds.), pp. 393-397, Academic Press, London.
25. 25. Tomme, P. (1991) Cellulasen uit Trichoderma reesei en Clostridium thermocellum: domeinstruktuur, chemische modificaties en mutagenese, Doctoral thesis, Rijksuniversiteit Gent, Belgium.
26. 26. Miller, G. L. (1959) Use of dinitrosalisylic acid reagent for determination of reducing sugar. Anal. Chem. 31, 426-428.
27. 27. Bradford, M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal.Biochem. 72, 248-254.
28. 28. Wetlaufer, D. B. (1962) Ultraviolet spectra of proteins and amino acids, in "Advances in Protein Chemistry" (Anson, M. L., Bailey, K., and Edsall, J. T., Eds.), Vol. 17, pp. 303-390, Academic Press, New York.
29. 29. Saloheimo, M., Lehtovaara, P., Penttilä, M., Teeri, T. T., Stâhlberg, J., Johansson, G., Pettersson, G., Claeyssens, M., Tomme, P., and Knowles, J. K. C. (1988) EGIII, a new endoglucanase from Trichoderma reesei: The characterization of both gene and enzyme. Gene 63, 11-21.
30. 30. Ruohonen, L., Koivula, A., Reinikainen, T., Valkeajärvi, A., Teleman, A., Claeyssens, N., Szardenings, M., Jones, T. A., and Teeri, T. T. (1993) Active site of T. reesei cellobiohydrolase II, in "Trichoderma reesei Cellulases and Other Hydrolases" (Suominen, P., and Reinikainen, T., Eds.). Vol. 8., pp. 87-96. Foundation for Biotechnical and Industrial Fermentation Research, Helsinki.
31. 31. Macarron, R., Acebal, C., Castillob, M. P., Dominquez, J. M., De La Mata, I., Pettersson, G., Toome, P., and Claeyssens, M. (1993). Mode of action of endoglucanase III from Trichoderma reesei. Biochem. J. 289, 867-873.
32. 32. Karhunen T., Mäntylä A., Nevalainen K. M. H., and Suominen P. L. (1993). High frequency one-step gene rejplacement in Trichoderma reesei. I. Endoglucanase I overproduction. Mol. Gen. Genet. 241, 515-522.
33. 33. Aho, S., and Paloheimo, M. (1990) The conserved terminal region of Trichoderma reesei cellulases forms a strong antigenic epitope for polyclonal antibodies. Biochim. Biophys. Acta 1087, 137-141.
34. 34. van Tilbeurgh, H. (1986) Studie van het cellulasecomplex uit Trichoderma reesei, Doctoral thesis, Rijksuniversiteit Gent, Belgium.