Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex

Journal of Cell Biology

Volumn 155, Issue 6, 2001, Pages 923-936

  Gleizes, Pierre Emmanuel ^a   Noaillac Depeyre, Jacqueline ^a   Léger Silvestre, Isabelle ^a   Teulières, Frédéric ^a   Dauxois, Jean Yves ^b   Pommet, Denys ^b   Azum Gelade, Marie Claude ^a   Gas, Nicole ^a  

^a CNRS   (France)

^b ENSAI Ecole Nationale de la Statistique et de lAnalyse de lInformation   (France)

Author keywords

Electron microscopy; Nuclear export; Nuclear pore complex; Ribosome; Yeast

Indexed keywords

GUANOSINE TRIPHOSPHATASE; NUCLEOPORIN; PROTEIN; RIBOSOME RNA; RNA HELICASE;

ARTICLE; ELECTRON MICROSCOPY; ENZYME ACTIVITY; FLUORESCENCE IN SITU HYBRIDIZATION; MEMBRANE TRANSPORT; MUTANT; MUTATION; NONHUMAN; NORTHERN BLOTTING; NUCLEOLUS; PHENOTYPE; PRIORITY JOURNAL; RIBOSOME; RNA PROCESSING; SACCHAROMYCES CEREVISIAE; ULTRASTRUCTURE;

ACTIVE TRANSPORT, CELL NUCLEUS; CALCIUM-BINDING PROTEINS; CYTOPLASM; FUNGAL PROTEINS; IN SITU HYBRIDIZATION; MICROSCOPY, ELECTRON; MUTAGENESIS; NUCLEAR PORE COMPLEX PROTEINS; NUCLEAR PROTEINS; RIBONUCLEOPROTEINS; RIBOSOMES; RNA, RIBOSOMAL; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0035842905     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200108142     Document Type: Article

References (61)

1. Nup120p: A yeast nucleoporin required for NPC distribution and mRNA transport
2. Degradation of ribosomal RNA precursors by the exosome
3. Guiding ribose methylation of rRNA
4. Functional analysis of Rrp7p, an essential yeast protein involved in pre-rRNA processing and ribosome assembly
5. Functional characterization of a Nup159p-containing nuclear pore subcomplex
6. Nucleolar localization of early t-RNA processing
7. A small nucleolar RNP protein is required for pseudouridylation of eukaryotic ribosomal RNAs
8. Assembly of 5S ribosomal RNA is required at a specific step of the pre-rRNA processing pathway
9. The product of the Saccharomyces cerevisiae RSS1 gene, identified as a high-copy suppressor of the rat7-1 temperature-sensitive allele of the RAT7/NUP159 nucleoporin, is required for efficient mRNA export
10. A structure/function analysis of Rat7p/Nup159p, an essential nucleoporin of Saccharomyces cerevisiae
11. C-terminal truncations of the yeast nucleoporin Nup145p produce a rapid temperature-conditional mRNA export defect and alterations to nuclear structure
12. From nucleoporins to nuclear pore complexes
13. Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking
14. Regulated nuclear localization of stress-responsive factors: How the nuclear trafficking of protein kinases and transcription factors contributes to cell survival
15. Nuclear export of 60s ribosomal subunits depends on Xpo1p and requires a nuclear export sequence-containing factor, Nmd3p, that associates with the large subunit protein Rpl10p
16. Pleiotropic nuclear defects associated with a conditional allele of the novel nucleoporin Rat9p/Nup85p
17. Transport between the cell nucleus and the cytoplasm
18. A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes
19. A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p
20. Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p
21. + RNA associated with mutation of the Saccharomyces cerevisiae RAT2/NUP120 gene
22. Nmd3p is a Crm1p-dependent adapter protein for nuclear export of the large ribosomal subunit
23. Rat8p/Dbp5p is a shutling transport factor that interacts with Rat7p/Nup159p and GIe1p and suppresses the mRNA export defect of xpo1-1 cells
24. The yeast RNA1 gene product necessary for RNA processing is located in the cytosol and apparently excluded from the nucleus
25. A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants
26. + RNA export
27. Two yeast nuclear pore complex proteins involved in mRNA export form a cytoplasmically oriented subcomplex
28. The GLFG repetitive region of the nucleoporin Nup116p interacts with Kap95p, an essential yeast nuclear import factor
29. Synthetic lethality with conditional dbp6 alleles identifies rsa1p, a nucleoplasmic protein involved in the assembly of 60S ribosomal subunits
30. Structural and functional analysis of the nucleolus of the fission yeast Schizosaccharomyces pombe
31. Functional compartmentalization of the nucleus in the budding yeast Saccharomyces cerevisiae
32. Specific binding of the karyopherin Kap121p to a subunit of the nuclear pore complex containing Nup53p, Nup59p, and Nup170p
33. Nucleocytoplasmic transport: The soluble phase
34. Nuclear export of the small tibosomal subunit requires the ran-GTPase cycle and certain nucleoporins
35. An RNA-export mediator with an essential nuclear export signal
36. NSP1: A yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain
37. A distinct nuclear import pathway used by ribosomal proteins
38. The yeast nuclear pore complex. Composition, architecture, and transport mechanism
39. Yeast heat shock mRNAs are exported through a distinct pathway defined by Rip1p
40. tRNA nuclear export in Saccharomyces cerevisiae: In situ hybridization analysis
41. Importin/karyopherin protein family members required for mRNA export from the nucleus
42. A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores
43. Structure and assembly of the Nup84p complex
44. Dbp5p/ Rat8p is a yeast nuclear pore-associated DEAD-box protein essential for RNA export
45. Factors affecting nuclear export of the 60S ribosomal subunit In vivo
46. The nuclear pore complex: From molecular architecture to functional dynamics
47. The RNA export factor Gle1p is located on the cytoplasmic fibrils of the NPC and physically interacts with the FG-nucleoporin Rip1p, the DEAD-box protein Rat8p/Dbp5p and a new protein Ymr 255p
48. Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export
49. Fungal small nuclear ribonucleoproteins share properties with plant and vertebrate U-snRNPs
50. Function and synthesis of small nucleolar RNAs
51. The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast
52. Maturation of ribosomes in yeast. I. Kinetic analysis by labelling of high molecular weight rRNA species
53. The cytoplasmic maturation of a ribosomal precursor in yeast
54. Processing of 20S pre-rRNA to 18S ribosomal RNA in yeast requires Rrp10p, an essential non-ribosomal cytoplasmic protein
55. Heat shock causes transient inhibition of yeast rRNA gene transcription
56. Ribosome synthesis in Saccharomyces cerevisiae
57. The economics of ribosome biosynthesis in yeast
58. Temperature sensitive mutations affecting ribosome synthesis in Saccharomyces cerevisiae
59. Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C
60. Karyopherins and kissing cousins