In Vitro Characterization of a Purified NS2/3 Protease Variant of Hepatitis C Virus

Journal of Biological Chemistry

Volumn 276, Issue 49, 2001, Pages 46678-46684

  Thibeault, Diane ^a   Maurice, Roger ^b   Pilote, Louise ^b   Lamarre, Daniel ^b   Pause, Arnim ^b  

^a BOEHRINGER INGELHEIM CANADA LTD   (Canada)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MEMBRANES; CELLS; GEL PERMEATION CHROMATOGRAPHY; GLYCEROL; PURIFICATION; VIRUSES;

CLEAVAGE;

ENZYMES;

1,10 PHENANTHROLINE; 1,7 PHENANTHROLINE; 4 (2 AMINOETHYL)BENZENESULFONYL FLUORIDE; ALANINE; APROTININ; CAPTOPRIL; EDETIC ACID; ENZYME VARIANT; IODOACETAMIDE; LEUCINE; LEUPEPTIN; METALLOPROTEINASE INHIBITOR; N [N (3 CARBOXYOXIRANE 2 CARBONYL)LEUCYL]AGMATINE; N ETHYLMALEIMIDE; PEPSTATIN; PHOSPHORAMIDON; POLYPROTEIN; PROTEINASE INHIBITOR; TOSYLLYSYL CHLOROMETHYL KETONE; TOSYLPHENYLALANYL CHLOROMETHYL KETONE; UNCLASSIFIED DRUG; VIRUS ENZYME; VIRUS PROTEASE NS2; VIRUS PROTEASE NS3; VIRUS PROTEIN; VIRUS PROTEIN NS4A; NS2 PROTEIN, HEPATITIS C VIRUS; NS3 PROTEIN, HEPATITIS C VIRUS;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BINDING SITE; CELL INCLUSION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; GEL FILTRATION CHROMATOGRAPHY; HEPATITIS C VIRUS; HYDROPHOBICITY; IN VITRO STUDY; MICROSOME MEMBRANE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN STRUCTURE; VIRUS INHIBITION; VIRUS REPLICATION; AMINO ACID SEQUENCE; ENZYMOLOGY; GEL CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ANIMALIA; ESCHERICHIA COLI; HEPATITIS C VIRUS; RNA VIRUSES;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HEPACIVIRUS; IN VITRO; MOLECULAR SEQUENCE DATA; PROTEIN FOLDING; VIRAL NONSTRUCTURAL PROTEINS;

EID: 0035824669     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M108266200     Document Type: Article

References (36)

1. Choo, Q.-L., Kuo, G., Weiner, A. J., Overby, L. R., Bradley, D. W., and Houghton, M. (1989) Science 244, 359-362
2. Kuo, G., Choo, Q.-L., Alter, H. J., Gitnick, G. L., Redeker, A. G., Purcell, R. H., Miyamura, T., Dienstag, J. L., Alter, M. J., Stevens, C. E., Tegtmeyer, G. E., Bonino, F., Colombo, M., Lee, W.-E., Kuo, C., Berger, K., Shuster, J. R., Overby, L. R., Bradley, D. W., and Houghton, M. (1989) Science 244, 362-364
3. Santolini, E., Migliaccio, G., and La Monica, N. (1994) J. Virol. 68, 3631-3641
4. Lin, C., Lindenbach, B. D., Pragai, B. M., McCourt, D. W., and Rice, C. M. (1994) J. Virol. 68, 5063-5073
5. Houghton, M., Weiner, A., Han, J., Kuo, G., and Choo, Q.-L. (1991) Hepatology 14, 381-388
6. Hijikata, M., Mizushima, H., Tanji, Y., Komoda, Y., Hirowatari, Y., Akagi, T., Kato, N., Kimura, K., and Shimotohno, K. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 10773-10777
7. Mizushima, H., Hijikata, M., Asabe, S., Hirota, M., Kimura, K., and Shimotohno, K. (1994) J. Virol. 68, 6215-6222
8. De Francesco, R., and Steinkühler, C. (2000) Curr. Top. Microbiol. Immunol. 242, 149-169
9. Dymock, B. W., Jones, P. S., and Wilson, F. X. (2000) Antiviral Chem. Chemother. 11, 79-96
10. Hong, Z., Standring, D. N., Baroudy, B., and Lau, J. Y. (2000) Acta Gastroenterologica Belgica 63, 210-212
11. Walker, M. A. (1999) Drug Discovery Today 4, 518-529
12. Grakoui, A., McCourt, D. W., Wychowski, C., Feinstone, S. M., and Rice, C. M. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 10583-10587
13. Hijikata, M., Mizushima, H., Akagi, T., Mori, S., Kakiuchi, N., Kato, N., Tanaka, T., Kimura, K., and Shimotohno, K. (1993) J. Virol. 67, 4665-4675
14. Hirowatari, Y., Hijikata, M., Tanji, Y., Nyumoya, H., Mizushima, H., Kimura, K., Tanaka, T., Kato, N., and Shimotohno, K. (1993) Arch. Virol. 133, 349-356
15. Pieroni, L., Santolini, E., Fipaldini, C., Pacini, L., Migliaccio, G., and La Monica, N. (1997) J. Virol. 71, 6373-6380
16. Reed, K., E., Grakoui, A., and Rice, C. M. (1995) J. Virol. 69, 4127-4136
17. Kolykhalov, A. A., Mihalik, K., Feinstone, S. M., and Rice, C. M. (2000) J. Virol. 74, 2046-2051
18. Liu, Q., Bhat, R. A., Prince, A. M., and Zhang, P. (1999) Biochem. Biophys. Res. Commun. 254, 572-577
19. Santolini, E., Pacini, L., Fipaldini, C., Migliaccio, G., and La Monica, N. (1995) J. Virol. 69, 7461-7471
20. Gorbalenya, A. E., and Snijder, E. J. (1996) Perspect. Drug Discovery Design. 6, 64-86
21. Bodansky, M. (1993) Peptide Chemistry, 2nd Edition, Springer-Verlag, Berlin
22. Darke, P. L., Jacobs, A. R., Waxman, L., and Kuo, L. C. (1999) J. Biol. Chem. 274, 34511-34514
23. Komoda, Y., Hijikata, M., Tanji, Y., Hirowatari, Y., Mizushima, H., Kimura, K. and Shimotohno, K. (1994) Gene (Amst.) 145, 221-226
24. Lin, T.-Y., and Timasheff, S. N. (1996) Protein Sci. 5, 372-381
25. Buchanan, S. K. (1999) Curr. Opin. Struct. Biol. 9, 455-461
26. Steinkühler, C., Urbani, A., Tomei, L., Biasiol, G., Sardana, M., Bianchi, E., Pessi, A., and De Francesco, R. (1996) J. Virol. 70, 6694-6700
27. Kim, J. L., Morgenstern, K. A., Lin, C., Fox, T., Dwyer, M. D., Landro, J. A., Chambers, S. P., Markland, W., Lepre, C. A., O'Malley, E. T., Harbeson, S. L., Rice, C. M., Murcko, M. A., Caron, P. R., and Thomson, J. A. (1996) Cell 87, 343-355
28. Yan, Y., Li, Y., Munshi, S., Sardana, V., Cole, J. L., Sardana, M., Steinkühler, C., Tomei, L., De Francesco, R., Kuo, L. C., and Chen, Z. (1998) Protein Sci. 7, 837-847
29. Bartenschlager, R., Ahlborn-Laake, L., Yasargil, K., Mous, J., and Jacobsen, H. (1995) J. Virol. 69, 7519-7528
30. Llinàs-Brunet, M., Bailey, M., Fazal, G., Goulet, S., Halmos, T., LaPlante, S., Maurice, R., Poirier, M., Poupart, M.-A., Thibeault, D., Wernie, D., and Lamarre, D. (1998) Bioorg. Med. Chem. Lett. 8, 1713-1718
31. Steinkühler, C., Biasiol, G., Brunetti, M., Urbani, A., Koch, U., Cortese, R., Pessi, A., and De Francesco, R. (1998) Biochemistry 37, 8899-8905
32. Tijms, M. A., van Dinten, L. C., Gorbalenya, A. E., and Snijder, E. J. (2001) Proc. Natl. Acad. Sci. U. S. A. 98, 1889-1894
33. Ziebuhr, J., Snijder, E. J., and Gorbalenya, A. E. (2000) J. Gen. Virol. 81, 853-879
34. Lohmann, V., Körner, F., Koch, J.-O., Herian, U., Theilmann, L., and Bartenschlager, R. (1999) Science 285, 110-113
35. Liu, X., Ropp, S. L., Jackson, R. J., and Frey, T. K. (1998) J. Virol. 72, 4463-4466
36. Liu, X., Yang, J., Mohamad Ghazi, A. M., and Frey, T. K. (2000) J. Virol. 74, 5949-5956