Plasmid transcriptional repressor copG oligomerises to render helical superstructures unbound and in complexes with oligonucleotides

Journal of Molecular Biology

Volumn 310, Issue 2, 2001, Pages 403-417

  Costa, M ^a   Sola M ^a   Del Solar, G ^b   Eritja, R ^a   Hernandez Arriaga A M ^b   Espinosa, M ^b   Gomis Ruth F X ^a   Coll, M ^a  

^a INSTITUT DE BIOLOGIA MOLECULAR DE BARCELONA   (Spain)

^b CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

Author keywords

CopG; Plasmid; Protein DNA complex; Transcriptional repressor; X ray crystal structure

Indexed keywords

DOUBLE STRANDED DNA; GENE PRODUCT; PROTEIN COPG; REPLICATION PROTEIN A; TETRAMER; UNCLASSIFIED DRUG;

ALPHA HELIX; ARTICLE; CRYSTAL STRUCTURE; DNA HELIX; NONHUMAN; NUCLEOTIDE REPEAT; NUCLEOTIDE SEQUENCE; OLIGOMERIZATION; PLASMID; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DNA INTERACTION; PROTEIN POLYMERIZATION; PROTEIN PROTEIN INTERACTION; STREPTOCOCCUS; STRUCTURE ANALYSIS; TRANSCRIPTION REGULATION;

EID: 0035816213     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4760     Document Type: Article

References (56)

1. Plasmid copy number control: An ever-growing story
2. Agrobacterium tumefaciens T-complex transport apparatus: A paradigm for a new family of multifunctional transporters in eubacteria
3. Control of plasmid regulation
4. Mechanisms that contribute to the stable segregation of plasmids
5. Plasmid incompatibility
6. The copy number of plasmid pLS1 is regulated by two trans-acting plasmid products: The antisense RNA II and the repressor protein
7. Replication control of plasmid pLS1: The antisense RNA II and the compact rnaII region are involved in translational regulation of the initiator RepB synthesis
8. Purification and characterization of RepA, a protein involved in the copy number control of plasmid pLS1
9. Plasmid pLS1-encoded RepA protein regulates transcription from repAB promoter by binding to a DNA sequence containing a 13-base-pair symmetric element
10. Chemical synthesis of a fully active transcriptional repressor protein
11. Replication control of plasmid pLS1: Efficient regulation of plasmid copy number is exerted by the combined action of two plasmid components, CopG and RNA II
12. Induced bending of plasmid pLS1 DNA by the plasmid-encoded protein RepA
13. Replication and control of circular bacterial plasmids
14. The structure of plasmid-encoded transcriptional repressor CopG unliganded and bound to its operator
15. The DNA loop model for ara repression: AraC protein occupies the proposed loop sites in vivo and repression-negative mutations lie in the same sites
16. Protein-DNA recognition
17. DNA recognition by proteins with the helix-turn-helix motif
18. Three-dimensional crystal structures of Escherichia coli met repressor with and without corepressor
19. DNA recognition by β-sheets in the Arc repressor-operator crystal structure
20. Crystal structure of the met repressor-operator complex at 2.8 Å resolution reveals DNA recognition by β-strands
21. Structure of Arc repressor in solution: Evidence for a family of β-sheet DNA binding proteins
22. Solution structure of dimeric Mnt repressor
23. Specific binding of TraY protein to oriT and the promoter region for the traY gene of plasmid R100
24. The F plasmid TraY gene product binds DNA as a monomer or a dimer-structural and functional implications
25. oriT-processing and regulatory roles of TrwA protein in plasmid R388 conjugation
26. Crystal structure of a CAP-DNA complex: The DNA is bent by 90°
27. DNA bending and its relation to nucleosome positioning
28. DNA recognition code of transcription factors
29. Structure of the nucleosome particle at 2.8 Å resolution
30. Clues and consequences of DNA bending in transcription
31. Specific β-sheet interactions
32. 3 Å resolution structure of a protein with histone like properties in prokaryotes
33. A protein structural motif that bends DNA
34. Histone-like proteins of bacteria
35. DNA ring closure mediated by protein HU
36. Escherichia coli OxyR protein represses the unmethylated bacteriophage Mu mom operon without blocking binding of the transcriptional activator C
37. Leucine-responsive regulatory protein: A global regulator of gene expression in E. coli
38. An Lrp-type transcriptional regulator from Agrobacterium tumefaciens condenses more than 100 nucleotides of DNA into globular nucleoprotein complexes
39. A novel nucleoprotein complex at a replication origin
40. Phage Φ29 protein p6 is in a monomer-dimer equilibrium that shifts to higher association states at the millimolar concentrations found in vivo
41. Overexpression, purification, crystallization and preliminary X-ray diffraction analysis of the pMV158-encoded plasmid transcriptional repressor protein CopG
42. Macromolecular data processing
43. The CCP4 suite: Programs for protein crystallography
44. AMoRe: An automated package for molecular replacement
45. Recognition by Max of its cognate DNA through a dimeric b/HLH/Z domain
46. Three-dimensional structure of the Stat3β homodimer bound to DNA
47. Turbo-Frodo
48. Crystallography & NMR System: A new software suite for macromolecular structure determination
49. GRASP: Graphical representation and analysis of surface properties
50. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
51. An extensively modified version of Molscript that includes greatly enhanced coloring capabilities
52. Raster3D version 2.0. A program for photorealistic molecular graphics
53. The definition of generalised helicoidal parameters and of axis curvature for irregular nucleic acids
54. WHAT IF: A molecular modelling and drug design program
55. Structure predictions of B-DNA duplex based on coordinates of the phosphorus atoms
56. An algorithm to generate DNA double helical molecule that follows any smooth spatial curve in 3-D