Overexpression, purification, crystallization and preliminary X-ray diffraction analysis of the pMV158-encoded plasmid transcriptional repressor protein CopG

FEBS Letters

Volumn 425, Issue 1, 1998, Pages 161-165

  Gomis Rüth, F Xavier ^a   Solà, María ^a   Pérez Luque, Rosa ^a   Acebo, Paloma ^b   Alda, M Teresa ^b   González, Ana ^c   Espinosa, Manuel ^b   Del Solar, Gloria ^b   Coll, Miquel ^a  

^a CSIC   (Spain)

^b CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^c DESY   (Germany)

Author keywords

CopG; Crystallization; Plasmid; Transcriptional repressor protein; X ray analysis

Indexed keywords

REPRESSOR PROTEIN;

ARTICLE; CRYSTALLIZATION; ESCHERICHIA COLI; PLASMID; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; TECHNIQUE; X RAY DIFFRACTION;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; DNA HELICASES; DNA, BACTERIAL; DNA-BINDING PROTEINS; ESCHERICHIA COLI; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; PLASMIDS; PROTEIN STRUCTURE, SECONDARY; PROTEINS; RECOMBINANT PROTEINS; REPRESSOR PROTEINS; TRANS-ACTIVATORS; X-RAY DIFFRACTION;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0032549691     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(98)00219-1     Document Type: Article

References (25)

1. Nordström K. Plasmid. 9:1983;1-7.
2. Novick R.P. Annu. Rev. Microbiol. 43:1989;537-565.
3. Espinosa M., del Solar G., Rojo F., Alonso J.C. FEMS Microbiol. Lett. 130:1995;111-120.
4. del Solar G., Espinosa M. Mol. Microbiol. 6:1992;83-94.
5. Brantl S. Mol. Microbiol. 14:1994;473-483.
6. del Solar G., Acebo P., Espinosa M. Mol. Microbiol. 18:1995;913-924.
7. del Solar G., de la Campa A.G., Pérez-Martín J., Choli T., Espinosa M. Nucleic Acids Res. 17:1989;2405-2420.
8. del Solar G., Pérez-Martín J., Espinosa M. J. Biol. Chem. 265:1990;12569-12575.
9. Kwak J.-H., Weisblum B. J. Bacteriol. 176:1994;5044-5051.
10. Pérez-Martín J., del Solar G., Lurz R., de la Campa A.G., Dobrinski B., Espinosa M. J. Biol. Chem. 264:1989;21334-21339.
11. Lee D.H., Schleif R.F. Proc. Natl. Acad. Sci. USA. 86:1989;476-480.
12. Ebright R.H. Mol. Microbiol. 8:1993;797-802.
13. Busby S., Ebright R.H. Mol. Microbiol. 23:1997;853-859.
14. Studier F.W., Rosenberg A.H., Dunn J.J., Dubendorff J.W. Methods Enzymol. 185:1990;60-89.
15. Ballester S., Alonso J.C., López P., Espinosa M. Gene. 86:1990;71-79.
16. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
17. Leslie, A.G.W. (1991) in: D. Moras, A.D. Podjanry and J.C. Thierry (Eds.), Crystallographic Computing V, Oxford University Press, Oxford, pp. 27-38.
18. CCP4 (1994) Acta Crystallogr. Sect. D, 50, 760-763.
19. Carter C.W.J., Carter C.W. J. Biol. Chem. 254:1979;12219-12223.
20. Jancarik J., Kim S.-H. J. Appl. Crystallogr. 24:1991;409-411.
21. Matthews B.W. J. Mol. Biol. 33:1968;491-497.
22. Navaza J. Acta Crystallogr. Sect. A. 50:1994;157-163.
23. Lacks S.A., López P., Geenberg B., Espinosa M. J. Mol. Biol. 192:1986;753-765.
24. Priebe S.D., Lacks S.A. J. Bacteriol. 171:1989;4778-4784.
25. del Solar G., Albericio F., Eritja R., Espinosa M. Proc. Natl. Acad. Sci. USA. 91:1994;5178-5182.