The F plasmid traY gene product binds DNA as a monomer or a dimer: Structural and functional implications

Molecular Microbiology

Volumn 20, Issue 6, 1996, Pages 1179-1187

  Nelson, William C ^a   Matson, Steven W ^a  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; DNA BINDING PROTEIN; GENE PRODUCT;

ARTICLE; BACTERIAL GENETICS; BACTERIUM CONJUGATION; BINDING SITE; DELETION MUTANT; DNA STRUCTURE; DNA TRANSFER; ESCHERICHIA COLI; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN PURIFICATION;

AMINO ACID SEQUENCE; BASE SEQUENCE; DNA, BACTERIAL; DNA-BINDING PROTEINS; ESCHERICHIA COLI PROTEINS; GLUTATHIONE TRANSFERASE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0029899661     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2958.1996.tb02638.x     Document Type: Article

References (27)

1. Bowie, J.U., and Sauer, R.T. (1990) TraY proteins of F and related episomes are members of the Arc and Mnt repressor family. J Mol Biol 211: 5-6.
2. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-252.
3. Breg, J.N., van Opheusden, J.H.J., Burgering, M.J.M., Boelens, R., and Kaptein, R. (1990) Structure of Arc repressor in solution: evidence for a family of β-sheet DNA-binding proteins. Nature 346: 586-589.
4. Clewell, D.B. (1993) Bacterial Conjugation. New York: Plenum Press.
5. Everett, R., and Willetts, N. (1980) Characterisation of an in vivo system for nicking at the origin of conjugal DNA transfer of the sex factor F. J Mol Biol 136: 129-150.
6. Frost, L.S., Ippen-Ihler, K., and Skurray, R.A. (1994) Analysis of the sequence and gene products of the transfer region of the F sex factor. Microbiol Rev 58: 162-210.
7. Fu, Y.-H.F., Tsai, M.-M., Luo, Y., and Deonier, R.C. (1991) Deletion analysis of the F plasmid oriT locus. J Bacteriol 173: 1012-1020.
8. Grandoso, G., Llosa, M., Zabala, J.C., and de la Cruz, F. (1994) Purification and biochemical characterization of TrwC, the helicase involved in plasmid R388 conjugal DNA transfer. Eur J Biochem 226: 403-412.
9. Hope, I.A., and Struhl, K. (1987) GCN4, a eukaryotic transcriptional activator protein, binds as a dimer to target DNA. EMBO J 6: 2781-2784.
10. Howard, M.T., Nelson, W.C., and Matson, S.W. (1995) Stepwise assembly of a relaxosome at the F plasmid origin of transfer. J Biol Chem 270: 28381-28386.
11. Inamoto, S., Yoshioka, Y., and Ohtsubo, E. (1988) Identification and characterization of the products from the traJ and traY genes of plasmid R100. J Bacteriol 170: 2749-2757.
12. Knight, K.L., Bowie, J.U., Vershon, A.K., Kelley, R.D., and Sauer, R.T. (1989) The Arc and Mnt Repressors. A new class of sequence-specific DNA-binding protein. J Biol Chem 264: 3639-3642.
13. Lahue, E.E., and Matson, S.W. (1990) Purified Escherichia coli F-factor TraY protein binds oriT. J Bacteriol 172: 1385-1391.
14. Lanka, E., and Wilkins, B.M. (1995) DNA processing reactions in bacterial conjugation. Ann Rev Biochem 64: 141-69.
15. Llosa, M., Grandoso, G., and de la Cruz, F. (1995) Nicking activity of TrwC directed against the origin of transfer of the IncW plasmid R388. J Mol Biol 246: 54-62.
16. Luo, Y., Gao, Q., and Deonier, R.C. (1994) Mutational and physical analysis of F plasmid traY protein binding to oriT. Mol Microbiol 11: 459-469.
17. McIntire, S., and Willetts, N. (1980) Transfer-deficient cointegrates of Flac and lambda prophage. Mol Gen Genet 178: 165-172.
18. Matson, S.W., and Morton, B.S. (1991) Escherichia coli DNA helicase I catalyzes a site- and strand-specific nicking reaction at the F plasmid oriT. J Biol Chem 266: 16232-16237.
19. Nelson, W.C., Morton, B.S., Lahue, E.E., and Matson, S.W. (1993) Characterization of the Escherichia coli F factor traY gene product and its binding sites. J Bacteriol 175: 2221-2228.
20. Nelson, W.C., Howard, M.T., Sherman, J.A., and Matson, S.W. (1995) The traY gene product and integration host factor stimulate Escherichia coli DNA helicase I-catalysed nicking at the F plasmid oriT. J Biol Chem 270: 28374-28380.
21. Phillips, S.E.V., Manfield, I., Parsons, I., Davidson, B.E., Rafferty, J.B., Somers, W.S., Margarita, D., Cohen, G.N., Saint-Girons, I., and Stockley, P.G. (1989) Cooperative tandem binding of met repressor of Escherichia coli. Nature 341: 711-715.
22. Raumann, B.E., Brown, B.M., and Sauer, R.Y. (1994a) Major groove DNA recognition by β-sheets: the ribbon-helix-helix family of gene regulatory proteins. Curr Op Struct Biol 4: 36-43.
23. Raumann, B.E., Rould, M.A., Pabo, C.O., and Sauer, R.T. (1994b) DNA recognition by β-sheets in the Arc repressor-operator crystal structure. Nature 367: 754-757.
24. Reygers, U., Wessel, R., Müller, H., and Hoffmann-Berling, H. (1991) Endonuclease activity of Escherichia coli DNA helicase I directed against the transfer origin of the F factor. EMBO J 10: 2689-2694.
25. Somers, W.S., and Phillips, S.E.V. (1992) Crystal structure of the met repressor-operator complex at 2.8Å resolution reveals DNA recognition by β-strands. Nature 359: 387-393.
26. Vershon, K.K., Kelley, R.D., and Sauer, R.T. (1989) Sequence-specific binding of arc repressor to DNA. Effects of operator mutations and modifications. J Biol Chem 264: 3267-3273.
27. Yoshioka, Y., Ohtsubo, H., and Ohtsubo, E. (1987) Repressor gene finO in plasmids R100 and F: constitutive transfer of plasmid F is caused by insertion of IS3 into F finO. J Bacteriol 169: 619-623.