Immobilized prion protein undergoes spontaneous rearrangement to a conformation having features in common with the infectious form

EMBO Journal

Volumn 20, Issue 7, 2001, Pages 1547-1554

  Leclerc, Estelle ^a   Peretz, David ^b   Ball, Haydn ^b   Sakurai, Hiroshi ^a   Legname, Giuseppe ^b   Serban, Ana ^b   Prusiner, Stanley B ^b,c   Burton, Dennis R ^a   Williamson, R Anthony ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Conformational change; Phage display; Prion protein; Recombinant antibodies

Indexed keywords

ISOPROTEIN; MONOCLONAL ANTIBODY; PRION PROTEIN;

ARTICLE; CONFORMATIONAL TRANSITION; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN IMMOBILIZATION; PROTEIN PROTEIN INTERACTION; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS;

ANIMALS; ANTIBODIES, MONOCLONAL; MICE; PROTEIN CONFORMATION; PRPC PROTEINS; PRPSC PROTEINS; TIME FACTORS;

EID: 0035794531     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.7.1547     Document Type: Article

References (60)

1. Monoclonal antibodies to the cellular and scrapie prion proteins
2. Nearly ubiquitous tissue distribution of the scrapie agent precursor protein
3. Non-genetic propagation of strain-specific properties of scrapie prion protein
4. Identification of a protein that purifies with the scrapie prion
5. Evidence for synthesis of scrapie prion proteins in the endocytic pathway
6. Normal prion protein has an activity like that of superoxide dismutase
7. The anti-prion activity of congo-red
8. The scrapie-associated form of PrP is made from a cell surface precursor that is both protease- and phospholipase-sensitive
9. N-terminal truncation of the scrapie-associated form of PrP by lysosomal protease(s): Implications regarding the site of conversion of PrP to the protease-resistant state
10. Structure of the recombinant full-length hamster prion protein PrP(29-231): The N terminus is highly flexible
11. Prion protein (PrP) with amino-proximal deletions restoring susceptibility of PrP knockout mice to scrapie
12. Predicted α-helical regions of the prion protein when synthesized as peptides form amyloid
13. Transmissible familial Creutzfeldt-Jakob disease associated with five, seven and eight extra octapeptide coding repeats
14. Cell biology of the prion protein
15. Reversible conversion of monomeric human prion protein between native and flbrilogenic conformations
16. Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors
17. A synthetic peptide initiates Gerstmann-Straussler-Scheinker (GSS) disease in transgenic mice
18. Sc conformational change
19. Mouse polyclonal and monoclonal antibody to scrapie-associated fibril protein
20. Trafficking of the cellular isoform of the prion protein
21. Free copper ions in the cell?
22. NMR structure of the bovine prion protein
23. Raman spectroscopic study on the copper(II) binding mode of prion octapeptide and its pH dependence
24. Improving biosensor analysis
25. A cellular gene encodes scrapie PrP 27-30 protein
26. Insertion in prion protein gene in familial Creutzfeldt-Jakob disease
27. Conversion of α-helices into β-sheets features in the formation of the scrapie prion proteins
28. Copper stimulates endocytosis of the prion protein
29. A conformational transition at the N terminus of the prion protein features in formation of the scrapie isoform
30. Inherited prion disease with 144 base pair gene insertion. 1. Genealogical and molecular studies
31. Prion protein and the scrapie agent: In vitro studies in infected neuroblastoma cells
32. Molecular biology of prions diseases
33. Inherited prion diseases
34. Prion diseases and the BSE crisis
35. Further purification and characterization of scrapie prions
36. Prion protein biology
37. NMR structure of the mouse prion domain PrP(121-231)
38. NMR characterization of the full-length recombinant murine prion protein, mPrP(23-231)
39. Global analysis of a macromolecular interaction measured on BIAcore
40. Sc molecules with different conformations
41. Molecular determinants of the physicochemical properties of a critical prion protein region comprising residues 106-126
42. Spontaneous deamidation and isomerization of Asn 108 in prion peptide 106-126 and in full-length prion protein
43. Propagation of prion strains through specific conformers of the prion protein
44. Expression of amino terminally truncated PrP in the mouse leading to ataxia and specific cerebellar lesions
45. A prion protein cycles between the cell surface and an endocytic compartment in cultured neuroblastoma cells
46. A glycolipid-anchored prion protein is endocytosed via clathrin-coated pits
47. Scrapie prion protein contains a phosphatidylinositol glycolipid
48. Differential release of cellular and scrapie prion proteins from cellular membranes by phosphatidylinositol-specific phospholipase C
49. Prion protein selectively binds copper (II) ions
50. Evidence for the conformation of the pathologic isoform of the prion protein enciphering and propagating prion diversity
51. Copper binding to the prion protein: Structural implications of four identical cooperative binding sites
52. Strain-specific prion-protein conformation determined by metal ions
53. Brain copper content and cuproenzyme activity do not vary with prion expression level
54. Molecular genetics of transmissible spongiform encephalopathies
55. The role of PrP in pathogenesis of experimental scrapie
56. Circumventing tolerance to generate autologous monoclonal antibodies to the prion protein
57. Mapping the prion protein using recombinant antibodies
58. NMR solution structure of the human prion protein
59. Conformational transitions in peptides containing two putative α-helices of the prion protein
60. Physical studies of conformational plasticity in a recombinant prion protein