Microtubule-dependent assembly of the nuclear envelope in Xenopus laevis egg extract

European Journal of Cell Biology

Volumn 80, Issue 11, 2001, Pages 678-691

  Ewald, Andrea ^a   Zünkler, Corinna ^a   Lourim, David ^b   Dabauvalle, Marie Christine ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b UNIVERSITY OF CHICAGO   (United States)

Author keywords

In vitro assembly; Microtubules; Nuclear mebrane; Pore complex; Xenopus egg extract

Indexed keywords

KINESIN; NUCLEAR PROTEIN; PROTEIN INHIBITOR;

ANIMAL CELL; ARTICLE; CELL NUCLEUS MEMBRANE; CELL STRUCTURE; CELL VACUOLE; CHROMATIN; CONTROLLED STUDY; FEMALE; MALE; MEMBRANE FORMATION; MICROTUBULE ASSEMBLY; MITOSIS; NONHUMAN; NUCLEAR PORE COMPLEX; OOCYTE; PRIORITY JOURNAL; SPERM; XENOPUS LAEVIS;

ANIMALIA; XENOPUS LAEVIS;

EID: 0035694525     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: 10.1078/0171-9335-00207     Document Type: Article

References (107)

1. Transport pathways of macromolecules between the nucleus and the cytoplasm
2. The nuclear lamina is a meshwork of intermediate-type filaments
3. Conformational states of tubulin liganded to colchicine, tropolone methyl ether, and podophyllotoxin
4. Vitro assembly of prenucleolar bodies in Xenopus egg extract
5. Identification of a novel X-linked gene responsible for Emery-Dreifuss muscular dystrophy
6. Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs
7. Sequential recruitment of NPC proteins to the nuclear periphery at the end of mitosis
8. On the cell-free association of lamins A and C with metaphase chromosomes
9. Filamentous cross-bridges link intermediate filaments to the nuclear pore complexes
10. Stepwise reassembly of the nuclear envelope at the end of mitosis
11. Annulate lamellae: Comparison of antigenic epitopes of annulate lamellae membranes with the nuclear envelope
12. Characterization of the nucleolar protein, B-36, using monoclonal antibodies
13. Identification of NG,NG Dimethyl Arginine in a nuclear protein from the lower eucaryote Physarum polycephalum homologous to the major proteins of mammalian 40S ribonucleoprotein particles
14. Distinct egg membrane vesicles differing in binding and fusion properties contribute to sea urchin male pronuclear envelopes formed in vitro
15. Targeting of membranes to sea urchin sperm chromatin is mediated by a lamin B receptor-like integral membrane protein
16. High content of a nuclear pore complex protein in cytoplasmic annulate lamellae of Xenopus oocytes
17. Cytoplasmic annulate lamellae in cultured cells: Composition, distribution, and mitotic behavior
18. Assembly of nuclear pore complexes in Xenopus egg extract
19. Distribution of emerin during the cell cycle
20. Identification of a soluble precursor complex essential for nuclear pore assembly in vitro
21. Spontaneous assembly of pore complex-containing membranes ('annulate lamellae') in Xenopus egg extract in the absence of chromatin
22. r 68000 pore complex glycoprotein interfere with nuclear protein uptake in Xenopus oocytes
23. The nuclear pore complex
24. Microtubule polymerization dynamics
25. Temporal differences in the appearance of NEP-B78 and an LBR-like protein during Xenopus nuclear envelope reassembly reflect the ordered recruitment of functionally discrete vesicle types
26. Nuclear membrane dynamics and reassembly in living cells: Targeting of an inner nuclear membrane protein in interphase and mitosis
27. Preassembly of annulate lamellae in egg extracts inhibits nuclear pore complex formation, but not nuclear membrane assembly
28. A biochemical and immunological comparison of nuclear and cytoplasmic pore complexes
29. Cell cycle-dependent phosphorylation of nucleoporins and nuclear pore membrane protein Gp210
30. Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation
31. Cloning of a cDNA for lamina-associated polypeptide 2 (LAP2) and identification of regions that specify targeting to the nuclear envelope
32. An antibody against a glycosylated integral membrane protein of the Xenopus laevis nuclear pore complex: A tool for the study of pore complex membranes
33. Nuclear assembly
34. Lamins and lamin-associated proteins
35. Nuclear envelope breakdown in mammalian cells involves stepwise lamina disassembly and microtubule-driven deformation of the nuclear membrane
36. Detection of individual fluorescently labeled reovirions in living cells
37. Integral membrane proteins and dynamic organization of the nuclear envelope
38. Identification of a major polypeptide of the nuclear pore complex
39. Lamins A and C bind and assemble at the surface of mitotic chromosomes
40. Dimples, pores, star-rings, and thin rings on growing nuclear envelopes: Evidence for structural intermediates in nuclear pore complex assembly
41. Motors and membrane traffic
42. Nuclear protein import
43. Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts
44. Kinesin and dynein super-family proteins in organelle transport and cell division
45. Dynamic relocation of chromosomal protein HMG-17 in the nucleus is dependent on transcriptional activity
46. Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles
47. Mechanism of mitotic block and inhibition of cell proliferation by taxol at low concentrations
48. Use of drugs to study the role of microtubule assembly dynamics in living cells
49. Microtubule dynamics in living cells
50. Beyond nuclear transport: Ran-GTP as a determinant of spindle assembly
51. Interconversion of metaphase and interphase microtubuli arrays, as studied by the injection of centrosomes and nuclei into Xenopus eggs
52. The contribution of the nuclear envelope to eukaryotic cell complexity: Architecture and functional roles
53. Annulate lamellae: A last frontier in cellular organelles
54. Lamin assembly in vivo
55. Electroblotting of multiple gels: A simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose
56. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
57. Microtubule-based endoplasmic reticulum motility in Xenopus laevis: Activation of membrane-associated kinesin during development
58. MAN1, an inner nuclear membrane protein that shares the LEM domain with lamina-associated polypeptide 2 and emerin
59. Bidirectional membrane traffic between the endoplasmic reticulum and Golgi apparatus
60. Induction of nuclear envelope breakdown, chromosome condensation and spindle formation in cell-free extracts
61. Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components
62. I protein synthesis during meiotic maturation
63. Membrane-associated lamins in Xenopus egg extracts: Identification of two vesicle populations
64. Lamin-dependent nuclear envelope reassembly following mitosis: An argument
65. Chromatin binding and polymerization of the endogenous Xenopus egg lamins: The opposing effects of glycogen and ATP
66. Differential mitotic phosphorylation of proteins of the nuclear pore complex
67. Vimentin-associated mitotic vesicles interact with chromosomes in a lamin B- and phosphorylation-dependent manner
68. The inner nuclear membrane protein LAP1 forms a native complex with B-type lamins and partitions with spindle-associated mitotic vesicles
69. Microtubules and microtubule-associated proteins
70. Enzymes for microtubule-dependent motility
71. Homologies both in primary and secondary structure between nuclear envelope and intermediate filament proteins
72. Nuclear pore complex assembly studied with a biochemical assay for annulate lamellae formation
73. The dynamic properties and possible functions of nuclear lamins
74. Disassembly of the nucleus in mitotic extracts: Membrane vesicularization, lamin disassembly, and chromosome condensation are independent processes
75. Nuclear reconstitution in vitro: Stages of assembly around protein-free DNA
76. A lamin-independent pathway for nuclear envelope assembly
77. Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound Ran
78. Intracellular trafficking of emerin, the Emery-Dreifuss muscular dystrophy protein
79. Isolation and characterization of the Drosophila nuclear envelope otefin cDNA
80. Retrograde transport by the microtubule-associated protein MAP1C
81. Are membranes of the mitotic apparatus translocated by microtubules?
82. Characterization of the microtubule movement produced by sea urchin egg kinesin
83. The lamin B receptor (LBR) provides essential chromatin docking sites at the nuclear envelope
84. r 34000 nucleolar protein of the U3 RNP particle
85. A marine natural product inhibitor of kinesin motors
86. Vitro nuclear assembly with affinity-purified nuclear envelope precursor vesicle fractions, PV1 and PV2
87. Meiosis, mitosis and microtubule motors
88. Cytoplasmic dynein is a minus end-directed motor for membranous organelles
89. Taxol stabilizes microtubules in mouse fibroblast cells
90. Microtubule-mediated transport of incoming Herpes Simplex Virus 1 capsids to the nucleus
91. Nuclear lamins: Their structure, assembly, and interactions
92. Microtubule-dependent plus- and minus end-directed motilities are competing processes for nuclear targeting of adenovirus
93. Assembly of nuclear pore complexes and annulate lamellae promotes normal pronuclear development in fertilized mammalian oocytes
94. A chromatin binding site in the tail domain of nuclear lamins that interacts with core histones
95. Taxol affects nuclear lamina and pore complex organization and inhibits import of karyophilic proteins into the cell nucleus
96. PBC68: A nuclear pore complex protein that associates reversibly with the mitotic spindle
97. An octamer of histones in chromatin and free in solution
98. The design plan of kinesin motors
99. Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility
100. Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggs
101. A distinct vesicle population targets membranes and pore complexes to the nuclear envelope in Xenopus eggs
102. Regulation of nuclear envelope precursor functions during cell division
103. Nuclear membrane dynamics
104. The mechanism of action of colchicine. Colchicine binding properties of sea urchin tail outer doublet tubulin
105. A lamin B receptor in the nuclear envelope
106. Integral membrane proteins of the nuclear envelope are dispersed throughout the endoplasmic reticulum during mitosis
107. Nuclear lamin-binding proteins