Recent advances in the diagnosis of Lyme disease

Expert Review of Molecular Diagnostics

Volumn 1, Issue 4, 2001, Pages 413-427

  Van Dam, Alje P ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Follow up; Immunoblotting; Lyme disease; PCR serology

Indexed keywords

RECOMBINANT ANTIGEN;

BORRELIA INFECTION; DATA ANALYSIS; DIAGNOSTIC ACCURACY; DIAGNOSTIC ERROR; DIAGNOSTIC VALUE; HUMAN; LYME DISEASE; NONHUMAN; POLYMERASE CHAIN REACTION; REVIEW; SERODIAGNOSIS; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; BORRELIA BURGDORFERI; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; LYME DISEASE; MOLECULAR DIAGNOSTIC TECHNIQUES; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 0035558503     PISSN: 14737140     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (96)

1. Lyme disease
2. Molecular typing of Borrelia burgdorferi sensu lato: Taxonomic, epidemiological and clinical implications
3. Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi
4. Animal models of Lyme disease: Pathogenesis and immunoprophylaxis
5. Fate of Borrelia burgdorferi DNA in tissues of infected mice after antibiotic treatment
6. Quantitative detection of Borrelia burgdorferi by real-time PCR
7. Persistence of Borrelia burgdorferi in experimentally infected dogs after antibiotic treatment
8. PCR-based quantification of Borrelia burgdorferi organisms in canine tissues over a 500-day postinfection period
9. Detection of active infection in nonhuman primates with Lyme neuroborreliosis: Comparison of PCR, culture and a bioassay
10. Transmission of Borrelia garinii OspA serotype 4 to BALB/c mice by Ixodes ricinus ticks collected in the field
11. The cell-mediated immune response to Borrelia afzelii, B. garinii and B. burgdorferi in C57BL/6 mice is dependent on antigen specificity
12. Guidelines for laboratory evaluation in the diagnosis of Lyme disease
13. Test-treatment strategies for patients suspected of having Lyme disease: A cost-effectiveness analysis
14. Standardization of medium for culturing Lyme disease spirochetes
15. European Borrelia burgdorferi isolated from humans and ticks culture conditions and antibiotic susceptibility
16. Solitary erythema migrans: A clinical, laboratory and epidemiological study of 77 Dutch patients
17. Diagnosis of early Lyme disease by polymerase chain reaction amplification and culture of skin biopsies from erythema migrans lesions
18. Different genospecies of Borrelia burgdorferi are associated with distinct clinical manifestations of Lyme borreliosis
19. Isolation of Borrelia burgdorferi from biopsy specimens taken from healthy-looking skin of patients with Lyme borreliosis
20. Improving the yield of blood cultures for patients with early Lyme disease
21. Isolation of Borrelia burgdorferi sensu lato from blood of patients with erythema migrans
22. Cultivation and characterization of spirochetes from cerebrospinal fluid of patients with Lyme borreliosis
23. PCR in laboratory diagnosis of human Borrelia burgdorferi infections
24. Molecular detection of Borrelia burgdorferi in formalin-fixed, paraffin-embedded lesions of Lyme disease
25. No evidence for Borrelia burgdorferi-specific DNA in lesions of localized scleroderma
26. Polymerase chain reaction for detection of Borrelia burgdorferi DNA in skin lesions of early and late Lyme borreliosis
27. Detection of Borrelia burgdorferi sensu lato in lesional skin of patients with erythema migrans and acrodermatitis chronica atrophicans by ospA-specific PCR
28. Diagnostic value of PCR for detection of Borrelia burgdorferi DNA in clinical specimens from patients with erythema migrans and Lyme neuroborreliosis
29. Direct molecular typing of Borrelia burgdorferi sensu lato species in synovial samples from patients with lyme arthritis
30. A two year prospective study to compare culture and polymerase chain reaction amplification for the detection and diagnosis of Lyme borreliosis
31. Diagnostic value of PCR for detection of Borrelia burgdorferi in skin biopsy and urine samples from patients with skin borreliosis
32. Vaccination against Lyme disease with recombinant Borrelia burgdorferi outer-surface lipoprotein A with adjuvant
33. Detection of Borrelia burgdorferi DNA in urine samples and cerebrospinal fluid samples from patients with early and late Lyme neuroborreliosis by polymerase chain reaction
34. Comparison of two methods for detecting it. Synthesis of Borrelia burgdorferi-specific antibodies and PCR for diagnosis of Lyme neuroborreliosis
35. Invasion of the central nervous system by Borrelia burgdorferi in acute disseminated infection
36. The polymerase chain reaction in the diagnosis of Lyme neuroborreliosis
37. The polymerase chain reaction for the detection of Borrelia burgdorferi in human body fluids
38. Detection of Borrelia burgdorferi in biological samples using the polymerase chain reaction assay
39. PCR detection of Borrelia burgdorferi DNA in cerebrospinal fluid of Lyme neuroborreliosis patients
40. Nondifferentiation between Lyme disease spirochetes from vector ticks and human cerebrospinal fluid
41. Detection of Borrelia burgdorferi by nested polymerase chain reaction in cerebrospinal fluid and urine of children with neuroborreliosis
42. Polymerase chain reaction with the 30-kb circular plasmid of Borrelia burgdorferi B31 as a target for detection of the Lyme borreliosis agents in cerebrospinal fluid
43. An optimized PCR leads to rapid and highly sensitive detection of Borrelia burgdorferi in patients with Lyme borreliosis
44. Detection of Borrelia burgdorferi DNA by polymerase chain reaction in cerebrospinal fluid in Lyme neurobotreliosis
45. Detection of Borrelia burgdorferi DNA by polymerase chain reaction in synovial fluid from patients with Lyme arthritis
46. Detection of Borrelia burgdorferi by DNA amplification in synovial tissue samples from patients with Lyme arthritis
47. Target imbalance: Disparity of Borrelia burgdorferi genetic material in synovial fluid from Lyme arthritis patients
48. Bloodstream invasion in early Lyme disease: Results from a prospective, controlled, blinded study using the polymerase chain reaction
49. Simultaneous presence of different Borrelia burgdorferi genospecies in biological fluids of Lyme disease patients
50. Polymerase chain reaction detection of Lyme disease: Correlation with clinical manifestations and serologic responses
51. Detection of Borrelia burgdorferi-specific DNA in urine specimens from patients with erythema migrans before and after antibiotic therapy
52. Evaluation of the detection of Borrelia burgdorferi DNA in urine samples by polymerase chain reaction
53. Detection of Borrelia burgdorferi DNA in urine of patients with ocular Lyme borreliosis
54. Demonstration of Borrelia burgdorferi DNA in urine samples from healthy humans whose sera contain B. burgdorferi-specific antibodies
55. Polymerase chain reaction control of antibiotic treatment in dermatoborreliosis
56. Identification of LFA-1 as a candidate autoantigen in treatment-resistant Lyme arthritis
57. Detection of Borrelia burgdorferi by polymerase chain reaction in synovial membrane but not in synovial fluid from patients with persisting Lyme arthritis after antibiotic therapy
58. Lack of Borrelia burgdorferi DNA in synovial samples from patients with antibiotic treatment-resistant Lyme arthritis
59. Borrelia burgdorferi detected by culture and PCR in clinical relapse of disseminated Lyme borreliosis
60. Borrelia burgdorferi DNA in the urine of treated patients with chronic Lyme disease symptoms. A PCR study of 97 cases
61. Three species of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto, B. afzelii and B. garinii) identified from cerebrospinal fluid isolates by pulsed-field gel electrophoresis and PCR
62. Association of specific subtypes of Borrelia burgdorferi with hematogenous dissemination in early Lyme disease
63. Rapid and sensitive quantification of Borrelia burgdorferi-infected mouse tissues by continuous fluorescent monitoring of PCR
64. Sensitive detection of Borrelia burgdorferi sensu lato DNA and differentiation of Borrelia species by Light Cycler PCR
65. Routine diagnosis of Borrelia burgdorferi (sensu lato) infections using a real-time PCR assay
66. Laboratory diagnostic techniques for patients with early Lyme disease associated with erythema migrans: A comparison of different techniques
67. Serodiagnosis in early Lyme disease
68. Use of recombinant OspC from Borrelia burgdorferi for serodiagnosis of early Lyme disease
69. Improved immunoglobulin M serodiagnosis in Lyme borreliosis by using a μ-capture enzyme-linked immunosprbent assay with biotinylated Borrelia burgdorferi flagellin
70. Impact of strain heterogeneity on Lyme disease serology in Europe: Comparison of enzyme-linked immunosorbent assays using different species of Borrelia burgdorferi sensu lato
71. Western blotting in the serodiagnosis of Lyme disease
72. Antibodies against whole sonicated Borrelia burgdorferi spirochetes, 41-kilodalton flagellin and P39 protein in patients with PCR- or culture-proven late Lyme borreliosis
73. Immunoblot interpretation criteria for serodiagnosis of early Lyme disease
74. Accuracy of IgM immunoblotting to confirm the clinical diagnosis of early Lyme disease
75. Interpretation criteria for standardized Western blots for three European species of Borrelia burgdorferi sensu lato
76. A European multicenter study of immunoblotting in serodiagnosis of Lyme borreliosis
77. Serodiagnosis of Lyme disease: Accuracy of a two-step approach using a flagellin-based ELISA and immunoblotting
78. New laboratory guidelines for serologic diagnosis of Lyme disease: Evaluation of the two-test protocol
79. Evaluation of two-test serodiagnostic method for early Lyme disease in clinical practice
80. Lyme neuroborreliosis: A new sensitive diagnostic assay for it. Synthesis of Borrelia burgdorferi-specific immunoglobulin G, A and M
81. Comparison of three serologic assays for Lyme disease testing
82. Humoral immune response to outer surface protein C of Borrelia burgdorferi in Lyme disease: Role of the immunoglobulin M response in serodiagnosis of early infection
83. Enzyme-linked immunosorbent assay using recombinant OspC and the internal 14-kDa flagellin fragment for serodiagnosis of early Lyme disease
84. Advantage of recombinant borrelial proteins for serodiagnosis of neuroborreliosis
85. Borrelia burgdorferi P35 and P37 proteins, expressed in vivo, elicit protective immunity
86. The immunoglobulin (IgG) antibody response to OspA and OspB correlates with severe and prolonged Lyme arthritis and the IgG response to P35 correlates with mild and brief arthritis
87. Human antibody responses to VlsE antigenic variation protein of Borrelia burgdorferi
88. Sensitive and specific serodiagnosis of Lyme disease by enzyme-linked immunosorbent assay with a peptide based on an immunodominant conserved region of Borrelia burgdorferi vlsE
89. Antibody Response to IR6, a conserved immunodominant region of the VlsE lipoprotein, wanes rapidly after antibiotic treatment of Borrelia burgdorferi infection in experimental animals and in humans
90. Molecular characterization of a 35-kilodalton protein of Borrelia burgdorferi, an antigen of diagnostic importance in early Lyme disease
91. The Borrelia burgdorferi 37-kilodalton immunoblot band (P37) used in serodiagnosis of early lyme disease is the flaA gene product
92. Recombinant chimeric Borrelia proteins for diagnosis of Lyme disease
93. A first-tier rapid assay for the serodiagnosis of Borrelia burgdorferi infection
94. Recombinant immunoblot in the serodiagnosis of Lyme borreliosis. Comparison with indirect immunofluorescence and enzyme-linked immunosorbent assay
95. An improved recombinant IgG immunoblot for serodiagnosis of Lyme borreliosis
96. Comparison of immunodot and Western blot assays for diagnosing Lyme borreliosis