Polymerase chain reaction detection of Lyme disease: Correlation with clinical manifestations and serologic responses

American Journal of Clinical Pathology

Volumn 105, Issue 5, 1996, Pages 647-654

  Mouritsen, C Lars ^a   Wittwer, Carl T ^a,b   Litwin, Christine M ^a,b   Yang, Liming ^b   Weis, Janis J ^b   Martins, Thomas B ^a   Jaskowski, Troy D ^a   Hill, Harry R ^a,b  

^a ARUP LABORATORIES   (United States)

^b UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

Author keywords

Borrelia burgdorferi; Polymerase chain reaction; Rapid cycle amplification; Serum

Indexed keywords

ANTIBIOTIC AGENT; ANTIFUNGAL AGENT; BACTERIAL DNA; CEFTRIAXONE; DOXYCYCLINE; IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN M; OUTER MEMBRANE PROTEIN;

ADULT; ARTICLE; BORRELIA BURGDORFERI; CHILD; CLINICAL FEATURE; DIAGNOSTIC VALUE; DNA DETERMINATION; ENZYME IMMUNOASSAY; FEMALE; GENE AMPLIFICATION; HUMAN; IMMUNOBLOTTING; LYME DISEASE; MAJOR CLINICAL STUDY; MALE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SERODIAGNOSIS;

EID: 0029925221     PISSN: 00029173     EISSN: None     Source Type: Journal    
DOI: 10.1093/ajcp/105.5.647     Document Type: Article

References (15)

1. Steere AC. Lyme disease. N Engl J Med 1989;321:586-596.
2. Rahn DW, Malawista SE. Lyme disease: Recommendations for diagnosis and treatment. Ann Intern Med 1991;114:472-481.
3. Krause A, Burmester GR, Rensing A, et al. Cellular Immune reactivity to recombinant OspA and Flagellin from Borrelia burgdorferi in patients with Lyme borreliosis: Complexity of humoral and cellular immune responses. J Clin Invest 1992;90:1077-1084.
4. Magnarelli LA, Miller JN, Anderson JF, Riviere GR. Cross-reactivity of nonspecific treponemal antibody in serologic tests for Lyme disease. J Clin Microbiol 1990;28:1276-1279.
5. Schmitz JL, Powell CS, Folds JD. Comparison of seven commercial kits for detection of antibodies to Borrelia burgdorferi. Eur J Clin Microbiol Infect Dis 1993;12:419-424.
6. Bergstrom S, Bundoc VG, Barbour AG, Molecular analysis of linear plasmid-encoded major surface proteins, OspA and OspB, of the Lyme disease spirochete Borrelia burgdorferi. Mol Microbiol 1989;3:479-486.
7. Lebech AM, Hansen K. Detection of Borrelia burgdorferi DNA in urine samples and cerebrospinal fluid samples from patients with early and late Lyme neuroborreliosis by polymerase chain reaction. J Clin Microbiol 1992;30:1646-1653.
8. Liebling MR, Nishio MJ, Rodriguez A, Sigal LH, Jin T, Louie JS. The polymerase chain reaction for the detection of Borrelia burgdorferi in human body fluids. Arthritis Rheum 1993;36:665-675.
9. Wittwer CT, Fillmore GC, Hillyard DR. Automated polymerase chain reaction in capillary tubes with hot air. Nucl Acids Res 1989;17:4353-4357.
10. Wittwer CT, Reed GB, Ririe KM. Rapid cycle DNA amplification. In: Mullis K, Ferre F, Gibbs R. The polymerase chain reaction. Deerfield Beach, FL: Springer-Verlag, 1994, pp 174-181.
11. Nocton JJ, Dressler F, Rutledge BJ, Rys PN, Persing DH, Steere AC. Detection of Borrelia burgdorferi DNA by polymerase chain reaction in synovial fluid from patients with Lyme arthritis. N Engl J Med 1994;330:229-234.
12. Wittwer CT, Fillmore GC, Garling DJ. Minimizing the time required for DNA amplification by efficient heat transfer to small samples. Anal Biochem 1990;186:328-331.
13. Rychlik W, Spencer WJ, Rhoads RE. Optimization of the annealing temperature for DNA amplification in vitro. Nucl Acids Res 1990;18:6409-6412.
14. Wittwer CT, Garling DJ. Rapid cycle DNA amplification: Time and temperature optimization. Biotechniques 1991;10:76-83.
15. Leidtke W, Opalka B, Zimmermann CW, Schmid E. Different methods of sample preparation influence sensitivity of Mycobacterium tuberculosis and Borrelia burgdorferi PCR. PCR Methods and Applications 1994;301-304.