A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT

EMBO Journal

Volumn 20, Issue 3, 2001, Pages 457-465

  Ku, Gregory M ^a   Yablonski, Deborah ^b   Manser, Edward ^c   Lim, Louis ^c,d   Weiss, Arthur ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

^c INSTITUTE OF MOLECULAR AND CELL BIOLOGY   (Singapore)

^d UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

PAK; PIX; PKL; Signal transduction; TCR

Indexed keywords

ADAPTOR PROTEIN; GUANINE NUCLEOTIDE EXCHANGE FACTOR; LAT PROTEIN; P21 ACTIVATED KINASE 1; PAXILLIN; PROTEIN CDC42; PROTEIN KINASE SYK; PROTEIN KINASE ZAP 70; PROTEIN NCK; PROTEIN SLP 76; PROTEIN TYROSINE KINASE; RAC1 PROTEIN; RAS PROTEIN; T LYMPHOCYTE RECEPTOR; UNCLASSIFIED DRUG;

ANTIGEN RECOGNITION; ARTICLE; BINDING SITE; CONTROLLED STUDY; ENZYME ACTIVATION; GENE OVEREXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; LEUKEMIA CELL LINE; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; T LYMPHOCYTE ACTIVATION; WESTERN BLOTTING;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; AMINO ACID SEQUENCE; CARRIER PROTEINS; CELL CYCLE PROTEINS; GTPASE-ACTIVATING PROTEINS; GUANINE NUCLEOTIDE EXCHANGE FACTORS; HUMANS; JURKAT CELLS; LYMPHOCYTE ACTIVATION; MEMBRANE PROTEINS; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; ONCOGENE PROTEINS; PHOSPHOPROTEINS; PROTEIN-SERINE-THREONINE KINASES; PROTEIN-TYROSINE KINASES; RAC1 GTP-BINDING PROTEIN; RECEPTORS, ANTIGEN, T-CELL; SIGNAL TRANSDUCTION; T-LYMPHOCYTES; TRANSFECTION; ZAP-70 PROTEIN-TYROSINE KINASE;

EID: 0035254857     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.3.457     Document Type: Article

References (48)

1. Grf40, a novel Grb2 family member, is involved in T cell signaling through interaction with SLP-76 and LAT
2. Pak to the future
3. A novel regulator of p21-activated kinases
4. A tyrosine-phosphorylated protein that binds to an important regulatory region on the cool family of p21-activated kinase-binding proteins
5. T cell receptor engagement induces tyrosine phosphorylation of FAK and Pyk2 and their association with Lck
6. Interaction of the Nck adapter protein with p21-activated kinase (PAK1)
7. Regulation of PAK activation and the T cell cytoskeleton by the linker protein SLP-76
8. Phosphatidylinositol 3-kinase regulates raf1 through pak phosphorylation of serine 338
9. The Rho-family GTP exchange factor Vav is a critical transducer of T cell receptor signals to the calcium, ERK and NF-κB pathways
10. αPix stimulates p21-activated kinase activity through exchange factor-dependent and -independent mechanisms
11. SLP-76 and Vav function in separate, but overlapping pathways to augment interleukin-2 promoter activity
12. LAT is required for TCR-mediated activation of PLCγ1 and the Ras pathway
13. Vav is a regulator of cytoskeletal reorganization mediated by the T-cell receptor
14. RAFTK, a novel member of the focal adhesion kinase family, is phosphorylated and associates with signaling molecules upon activation of mature T lymphocytes
15. ras effector pathways regulate nuclear factor of activated T cells
16. At least two non-antigen-binding molecules are required for signal transduction by the T-cell antigen receptor
17. Defects in actin-cap formation in Vav-deficient mice implicate an actin requirement for lymphocyte signal transduction
18. Molecular cloning of SLP-76, a 76-kDa tyrosine phosphoprotein associated with Grb2 in T cells
19. Signal transduction by the TCR for antigen
20. Protein-tyrosine kinase Pyk2 is involved in interleukin-2 production by Jurkat T cells via its tyrosine 402
21. A guanine nucleotide exchange factor-independent function of Vav1 in transcriptional activation
22. Localization of LAT in glycolipid-enriched microdomains is required for T cell activation
23. The hematopoietic-specific adaptor protein gads functions in T-cell signaling via interactions with the SLP-76 and LAT adaptors
24. Tyrosine phosphorylation mediates both activation and downmodulation of the biological activity of Vav
25. Expression of constitutively active α-PAK reveals effects of the kinase on actin and focal complexes
26. PAK kinases are directly coupled to the PIX family of nucleotide exchange factors
27. Paxillin phosphorylation and association with Lck and Pyk2 in anti-CD3- or anti-CD45-stimulated T cells
28. Tyrosine phosphorylation of Pyk2 is selectively regulated by Fyn during TCR signaling
29. Regulation of Vav-SLP-76 binding by ZAP-70 and its relevance to TCR ζ/CD3 induction of interleukin-2
30. lck: A role for SLP-76 in CD4-induced desensitization of the TCR/CD3 signaling complex
31. Deficiency of PTEN in Jurkat T cells causes constitutive localization of Itk to the plasma membrane and hyperresponsiveness to CD3 stimulation
32. Regulation of the polarization of T cells toward antigen-presenting cells by Ras-related GTPase CDC42
33. Regulation of the protein kinase Raf-1 by oncogenic Ras through phosphatidylinositol 3-kinase, Cdc42/Rac and Pak
34. Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: A role in cytoskeletal remodeling
35. A requirement for the Rho-family GTP exchange factor Vav in positive and negative selection of thymocytes
36. Cytoskeletal polarization and redistribution of cell-surface molecules during T cell antigen recognition
37. T cell antigen-receptor signal transduction
38. Requirement for the coexpression of T3 and the T cell antigen receptor on a malignant human T cell line
39. The role of T3 surface molecules in the activation of human T cells: A two-stimulus requirement for IL 2 production reflects events occurring at a pretranslational level
40. Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: Reconstitution studies in a ZAP-70-deficient Jurkat T-cell line
41. Vav and SLP-76 interact and functionally cooperate in IL-2 gene activation
42. A Nck-Pak1 signaling module is required for T-cell receptor-mediated activation of NFAT, but not of JNK
43. Uncoupling of nonreceptor tyrosine kinases from PLC-γ1 in an SLP-76-deficient T cell
44. αPIX nucleotide exchange factor is activated by interaction with phosphatidylinositol 3-kinase
45. LAT: The ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation
46. LAT palmitoylation: Its essential role in membrane microdomain targeting and tyrosine phosphorylation during T cell activation
47. Functional analysis of LAT in TCR-mediated signaling pathways using a LAT-deficient Jurkat cell line
48. Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal complex disassembly