Multiple pathways in the trafficking and assembly of connexin 26, 32 and 43 into gap junction intercellular communication channels

Journal of Cell Science

Volumn 114, Issue 21, 2001, Pages 3845-3855

  Martin, P E M ^a   Blundell, G ^a   Ahmad, S ^a   Errington, R J ^a   Evans, W H ^a  

^a CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

Connexin fluorescent protein chimera; Gap junction; Trafficking pathways

Indexed keywords

AMINO ACID; BREFELDIN A; CONNEXIN 26; CONNEXIN 32; CONNEXIN 43; GREEN FLUORESCENT PROTEIN; MEMBRANE PROTEIN; NOCODAZOLE;

ARTICLE; CELL COMMUNICATION; CONTROLLED STUDY; GAP JUNCTION; GOLGI COMPLEX; HUMAN; HUMAN CELL; MUTATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN TARGETING; PROTEIN TRANSPORT; SIGNAL TRANSDUCTION;

ANIMALS; BIOLOGICAL TRANSPORT; BREFELDIN A; CERCOPITHECUS AETHIOPS; CONNEXIN 43; CONNEXINS; COS CELLS; CYTOSKELETON; ENDOPLASMIC RETICULUM; GAP JUNCTIONS; HELA CELLS; HUMANS; INTRACELLULAR MEMBRANES; NOCODAZOLE; RECOMBINANT FUSION PROTEINS; SIGNAL TRANSDUCTION;

MAMMALIA;

EID: 0035188530     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (62)

1. Synthesis and assembly of connexins in vitro into homomeric and heteromeric functional gap junction hemichannels
2. Assembly of gap junction channels. Identification of oligomeric intermediates and determinants and evidence of a role for calmodulin
3. Biochemistry, mutagenesis and oligomerisation of DSRed, a red fluorescent protein from coral
4. Isoform composition of connexin channels determines selectivity among second messengers and uncharged molecules
5. Clustering of connexin 43-enhanced green fluorescent protein gap junction channels and functional coupling in living cells
6. The role of selective transport in neuronal protein sorting
7. A quantitative analysis of connexin-specific permeability differences of gap junctions expressed in HeLa transfectants and Xenopus oocytes
8. Altered formation of hemichannels and gap junction channels caused by C-terminal connexin-32 mutations
9. Direct targets of phosphoinositide 3-kinase products in membrane traffic and signal transduction
10. Altered trafficking of mutant connexin32
11. Assembly of heteromeric connexons in guinea-pig liver en route to the Golgi apparatus, plasma membrane and gap junctions
12. Brefeldin A redistributes resident and itinerant Golgi proteins to the endoplasmic reticulum
13. Specific permeability and selective formation of gap junction channels in connexin-transfected HeLa-cells
14. Trafficking pathways leading to the formation of gap junctions
15. Connexin-specific distribution within gap junctions revealed in living cells
16. Membrane insertion of gap junction connexins - Polytopic channel-forming membrane-proteins
17. Cell-free synthesis and assembly of connexins into functional gap junction membrane channels
18. Rapid determination of gap junction formation using HeLa cells microinjected with cDNAs encoding wild-type and chimeric connexins
19. Connexin-aequorin chimerae report cytoplasmic calcium environments along trafficking pathways leading to gap junction biogenesis in living COS-7 cells
20. Intracellular trafficking pathways in the assembly of connexins into gap junctions
21. Selective transfer of endogenous metabolites through gap junctions composed of different connexins
22. Formation of heteromeric gap junction channels by connexins 40 and 43 in vascular smooth muscle cells
23. Dissecting the role of the Golgi complex and lipid rafts in biosynthetic transport of cholesterol to cell surfaces
24. Kinetic analysis of secretory proteins traffic and characterisation of Golgi to plasma membrane transport intermediates in living cells
25. Dynamics of gap junctions observed in living cells with connexin43-GFP chimeric protein
26. Heteromeric connexons in lens gap junction channels
27. Posttranslational phosphorylation of lens fiber connexin46 - A slow occurrence
28. Trafficking, assembly, and function of a connexin 43-green fluorescent protein chimera in live mammalian cells
29. Rapid appearance of connexin 26-positive gap-junctions in centrilobular hepatocytes without induction of messenger-RNA and protein-synthesis in isolated-perfused liver of female rat
30. Changes in cellular-distribution of connexin-32 and connexin-26 during formation of gap-junctions in primary cultures of rat hepatocytes
31. Connexin46 is retained as monomers in a trans-Golgi compartment of osteoblastic cells
32. The gap junction communication channel
33. Degradation of gap junctions and connexins
34. Gap junction turnover, intracellular trafficking and phosphorylation of connexin 43 in Brefeldin A-treated mammary turnover cells
35. Developmental expression and assembly of connexins into homomeric and heteromeric gap junction hemichannels in the mouse mammary gland
36. Lipids in gap junction assembly and function
37. Measurement of colocalisation of objects in dual-colour confocal microscopy
38. Trafficking and targeting to gap junctions of connexin 32 mutations to gap junctions in Charcot-Marie-Tooth X-linked disease
39. Assembly of chimeric connexin-aequorin proteins into functional gap junction channels. Reporting intracellular and plasma membrane calcium environments
40. Properties of connexin 26 gap junctional proteins derived from mutations associated with non-syndromal hereditary deafness
41. Analysis of gap junction assembly using mutated connexins detected in Charcot Marie Tooth X-linked disease
42. Targeting motifs and functional parameters governing the assembly of connexins into gap junctions
43. Rapid modulation of gap junction expression in mouse mammary-gland during pregnancy, lactation, and involution
44. Biochemical-analysis of connexin43 intracellular-transport, phosphorylation, and assembly into gap junctional plaques
45. Multisubunit assembly of an integral plasma-membrane channel protein, gap junction connexin43, occurs after exit from the ER
46. Differential expression of connexins during neocortical development and neuronal circuit formation
47. The molecular basis of selective permeability of connexins is complex and includes both size and charge
48. Selective pemeability of different connexin channels to the second messenger inositol 1, 4, 5 - trisphosphate
49. Intercellular calcium waves in HeLa cells expressing GFP-labelled connexin 43, 32 or 26
50. Insulin action on GLUT4 traffic visualised in single 3T3-L1 adipocytes by using ultra-fast microscopy
51. Cyclic AMP and LDL trigger a rapid enhancement in gap junction assembly through a stimulation of connexin trafficking
52. Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular pathway to the ER
53. Brefeldin A acts to stabilise an abortive ARF-GDP-Sec7 domain protein complex: Involvement of specific residues of the SEC7 domain
54. A role for caveolin in transport of cholesterol from endoplasmic reticulum to plasma membrane
55. Negative physiology: What connexin-deficient mice reveal about functional roles of individual gap junction proteins
56. Acute phase response and circadian expression of connexin 26 are not altered in connexin 32-deficient mouse liver
57. Role of microtubules in the organisation of the Golgi complex
58. The subapical compartment; a novel sorting centre?
59. Intracellular transport, assembly and degradation of wild-type and disease linked mutant gap junction proteins
60. Microtubule-dependent transport of secretory vesicles visualised in real time with a GFP-tagged secretory protein
61. Synthesis, assembly and structure of gap junction intercellular channels
62. Membrane integration of in vitro - Translated gap junctional proteins: Co- and post translational mechanisms