Dynamics of gap junctions observed in living cells with connexin43-GFP chimeric protein

European Journal of Cell Biology

Volumn 78, Issue 12, 1999, Pages 856-866

  Holm, Ingvar ^a,b   Mikhailov, Alexei ^a,c   Jillson, Tracy ^a   Rose, Birgit ^a  

^a MARINE BIOLOGICAL LABORATORY   (United States)

^b KRISTIANSTAD UNIVERSITY   (Sweden)

^c MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

cAMP; Cell to cell channels; Connexin43; Gap junction; Green fluorescent protein; Timelapse videomicroscopy

Indexed keywords

CHIMERIC PROTEIN; CONNEXIN 43; CYCLIC AMP; GREEN FLUORESCENT PROTEIN;

ANIMAL CELL; ARTICLE; CELL CONTACT; CONTROLLED STUDY; EXPRESSION VECTOR; FLUORESCENCE MICROSCOPE; GAP JUNCTION; GENETIC TRANSFECTION; MEMBRANE CHANNEL; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; RAT; TIME;

ANIMALIA;

EID: 0033394620     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0171-9335(99)80087-9     Document Type: Article

References (45)

1. Adams, C.L., Y Chen, S.J. Smith, W.J. Nelson: Mechanisms of epithelial cell-cell adhesion and cell compaction revealed by high-resolution tracking of E-cadherin-green fluorescent protein. J. Cell Biol. 142, 1105-1110 (1998).
2. Beyer, E.C., D.L. Paul, D.A. Goodenough: Connexin43: a protein from rat heart homologous to a gap junction protein from liver. J. Cell Biol. 105, 2621-2629 (1987).
3. Beyer, E.C., J. Kistler, D.L. Paul, D.A. Goodenough: Antisera directed against connexin 43 peptides react with a 43-kD protein localized to gap junctions in myocardium and other tissues. J. Cell Biol. 108, 595-605 (1989).
4. Beyer, E.C., D.L. Paul, D.A. Goodenough: Connexin family of gap junction proteins. J. Membr. Biol. 116, 187-194 (1990).
5. Caspar, D.L., D.A. Goodenough, L. Makowski, W.C. Phillips: Gap junction structures. I. Correlated electron microscopy and x-ray diffraction. J. Cell Biol. 74, 605-628 (1977).
6. Chalfie, M., Y. Tu, G. Euskirchen, W.W. Ward, D.C. Prasher: Green fluorescent protein as a marker for gene expression. Science 263, 802-805 (1994).
7. Cole, N.B., C.L. Smith, N. Sciaky, M. Terasaki, M. Edidin, J. Lippincott-Schwartz: Diffusional mobility of Golgi proteins in membranes of living cells. Science 273, 797-801 (1996).
8. Cole, N.B., J. Ellenberg, J. Song, D. DiEuliis, J. Lippincott-Schwartz: Retrograde transport of Golgi-localized proteins to the ER. J. Cell Biol. 140, 1-15 (1998).
9. Craig, N.: Effect of reduced temperatures on protein synthesis in mouse L cells. Cell 4, 329-335 (1975).
10. Dermietzel, R., R. Leibstein, A. Frixen, U. Janssen-Timmen, O. Traub, K. Willecke: Gap junctions in several tissues share antigenic determinants with liver gap junctions. EMBO J. 3, 2261-2270 (1984).
11. Dermietzel, R., B. Yancey, U. Janssen-Timmen, O. Traub, K. Willecke, J.-P. Revel: Simultaneous light and electronmicroscopic observation of immunolabelled liver 27 kD gap junction protein on ultra-thin cryosections. J. Histochem. Cytochem. 35, 387-392 (1987).
12. Essner, J. J., J.G. Laing, E.G. Beyer, R.G. Johnson, P.B. Hackett, Jr: Expression of zebrafish connexin43.4 in the notochord and tail bud of wild-type and mutant no tail embryos. Dev. Biol. 177, 449-462 (1996).
13. Flagg-Newton, J.L., I. Simpson, W.R. Loewenstein: Permeability of the cell-to-cell membrane channels in mammalian cell junction. Science 205, 404-407 (1979).
14. George, C.H., J.M. Kendall, A.K. Campbell, W.H. Evans: Connexin-aequorin chimerae report cytoplasmic calcium environments along trafficking pathways leading to gap junction biogenesis in living COS-7 cells. J. Biol. Chem. 273, 29822-29829 (1998).
15. Goodenough, D. A., J.P. Revel: A fine structural analysis of intercellular junctions in the mouse liver. J. Cell Biol. 45, 272-290 (1970).
16. Hampton, R.Y., A. Koning, R. Wright, J. Rine: In vivo examination of membrane protein localization and degradation with green fluorescent protein. Proc. Natl. Acad. Sci. USA 93, 828-833 (1996).
17. [17) Hertzberg, E.L., R.V. Skibbens: A protein homologous to the 27,000 dalton liver gap junction protein is present in a wide variety of species. Cell 39, 61-69 (1984).
18. Ho, C.L., J.L. Martys, A. Mikhailov, G.G. Gundersen, R.K. Liem: Novel features of intermediate filament dynamics revealed by green fluorescent protein chimeras. J. Cell Sci. 111, 1767-1778 (1998).
19. Hofer, A., R. Dermietzel: Visualization and functional blocking of gap junction hemichannels (connexons) with antibodies against external loop domains in astrocytes. Glia 24, 141-154 (1998).
20. Janssen-Timmen, U., R. Dermietzel, U. Frixen, A. Leibstein, O. Traub, K. Willecke: Immunochemical localization of the gap junction 26 kDa protein in mouse liver plasma membranes. EMBO J. 2, 295-302 (1983).
21. Kumar, N.M., N.B. Gilula: Molecular biology and genetics of gap junction channels. Semin. Cell Biol. 3, 3-16 (1992).
22. Kumar, N.M., N.B. Gilula: The gap junction communication channel. Cell 84, 381-388 (1996).
23. Kumar, N.M., D.S. Friend, N.B. Gilula: Synthesis and assembly of human β1 gap junctions in BHK cells by DNA transfection with the human β1 cDNA. J Cell Sci. 108, 3725-3734 (1995).
24. Lippincott-Schwartz, J., C.L. Smith: Insights into secretory and endocytic membrane traffic using green fluorescent protein chimeras. Curr. Opin. Neurobiol. 7, 631-639 (1997).
25. Lippincott-Schwartz, J., N. Cole, J. Presley: Unravelling Golgi membrane traffic with green fluorescent protein chimeras. Trends Cell Biol. 8, 16-20 (1998).
26. Loewenstein, W.R.: Junctional intercellular communication: the cell-to-cell membrane channel. Physiol. Rev. 61, 829-913 (1981).
27. Makowski, L., D.L. Caspar, W.C. Phillips, D.A. Goodenough: Gap junction structures. II. Analysis of the x-ray diffraction data. J. Cell Biol. 74, 629-645 (1977).
28. Martin, P.E.M., C.H. George, C. Castro, J.M. Kendall, J. Capel, A.K. Campbell, A. Revilla, L.C. Barrio, W.H. Evans: Assembly of chimeric connexin-aequorin proteins into functional gap junction channels. Reporting intracellular and plasma membrane calcium environments. J. Biol. Chem. 273, 1719-1726 (1998).
29. Mehta, P.P., A. Hotz-Wagenblatt, B. Rose, D. Shalloway, W.R. Loewenstein: Incorporation of the gene for a cell-cell channel protein into transformed cells leads to normalization of growth. J. Membr. Biol. 124, 207-225 (1991).
30. Mehta, P.P., M. Yamamoto, B. Rose: Transcription of the gene for the gap junctional protein connexin43 and expression of functional cell-to-cell channels are regulated by cAMP. Mol. Biol. Cell 3, 839-850 (1992).
31. Musil, L.S., E. Beyer, D.A. Goodenough: Expression of the gap junction protein connexin43 in embryonic chick lens: molecular cloning, ultrastructural localization, and post-translational phosphorylation. J. Membr. Biol. 116, 163-175 (1990).
32. Musil, L.S., D.A. Goodenough: Biochemical analysis of connexin43 transport, phosphorylation and assembly into gap junctional plaques. J. Cell Biol. 115, 1357-1374 (1991).
33. Musil, L.S., D.A. Goodenough: Multisubunit assembly of an integral plasma membrane channel protein, gap junction connexin43, occurs after exit from the ER. Cell 74, 1065-1077 (1993).
34. Paul, D.L.: Molecular cloning of cDNA for rat liver gap junction protein. J. Cell Biol. 103, 123-134 (1986).
35. Pouli, A.E., H.J. Kennedy, J.G. Schofield, G.A.Rutter: Insulin targeting to the regulated secretory pathway after fusion with green fluorescent protein and firefly luciferase. Biochem. J. 331, 669-675 (1998).
36. Prasher, D.C., V.K. Eckenrode, W.W. Ward, F.G. Prendergast, M.J. Cormier: Primary structure of Aequorea victoria green-fluorescent protein. Gene 111, 229-233 (1992).
37. Revel, J.P., M.J. Karnovsky: Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. J. Cell Biol. 33, C7-C12 (1967).
38. Saez, J.Z., W.A. Gregory, T. Watanabe, R. Dermietzel, E.L. Hertzberg, I. Reid, M.V.L. Bennett, D.C. Spray: cAMP delays disappearance of gap junctions between pairs of hepatocytes in primary culture. Am. J. Physiol. 257, C1-C11 (1989).
39. Sciaky, N., J. Presley, C. Smith, K.J. Zaal, N. Cole, J.E. Moreira, M. Terasaki, E. Siggia, J. Lippincott-Schwartz: Golgi tubule traffic and the effects of brefeldin A visualized in living cells. J. Cell Biol. 139, 1137-1155 (1997).
40. Stamminger, T., H. Fickenscher, B. Fleckenstein: Cell type-specific induction of the major immediate early enhancer of human cytomegalovirus by cyclic AMP. J. Gen. Virol. 71, 105-113 (1990).
41. Sullivan, R., C.W. Lo: Expression of a connexin43/galactosidase fusion protein inhibits gap junctional communication in NIH3T3 cells. J. Cell Biol. 130, 419-429 (1995).
42. Unwin, P.N., G. Zampighi: Structure of the junction between communicating cells. Nature 283, 545-549 (1980).
43. Unwin, P.N., P.D. Ennis: Two configurations of a channel-forming membrane protein. Nature 307, 609-613 (1984).
44. Wang, Y., B. Rose: Clustering of Cx43 cell-to-cell channels into gap junction plaques: regulation by cAMP and microfilaments. J. Cell Sci. 108, 3501-3508 (1995).
45. Wolff, J.R., K. Stuke, M. Missler, H. Tytko, P. Schwarz, A. Rohlmann, T.I. Chao: Autocellular coupling by gap junctions in cultured astrocytes: a new view on cellular autoregulation during process formation. Glia 24, 121-140 (1998).