Ecteinascidin 743 induces protein-linked DNA breaks in human colon carcinoma HCT116 cells and is cytotoxic independently of topoisomerase I expression

Clinical Cancer Research

Volumn 7, Issue 1, 2001, Pages 185-191

  Takebayashi, Yuji ^a   Goldwasser, François ^a   Urasaki, Yoshimasa ^a   Kohlhagen, Glenda ^a   Pommier, Yves ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

3' (3 CYANOMORPHOLINO) 3' DEAMINODOXORUBICIN; 3' DEAMINO 3' MORPHOLINODOXORUBICIN; BISANTRENE; CAMPTOTHECIN; CHROMOMYCIN A3; CISPLATIN; DACTINOMYCIN; DAUNORUBICIN; DNA; DNA TOPOISOMERASE; DOXORUBICIN; ECHINOMYCIN; MITHRAMYCIN; NSC 648766; PHYLLANTHOSIDE; TRABECTEDIN; UNCLASSIFIED DRUG;

ARTICLE; CELL CYCLE PHASE; COLON CARCINOMA; CONTROLLED STUDY; CYTOTOXICITY; DNA ADDUCT; DNA DAMAGE; DNA STRAND BREAKAGE; DNA SYNTHESIS INHIBITION; DOSE RESPONSE; DRUG STRUCTURE; HUMAN; HUMAN CELL; LEUKEMIA; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN DNA INTERACTION; PROTEIN EXPRESSION;

ANTINEOPLASTIC AGENTS, ALKYLATING; CELL CYCLE; CELL DIVISION; CELL SURVIVAL; COLONIC NEOPLASMS; DIOXOLES; DNA DAMAGE; DNA TOPOISOMERASES, TYPE I; DNA, NEOPLASM; DOSE-RESPONSE RELATIONSHIP, DRUG; FLOW CYTOMETRY; FORMAZANS; HUMANS; IMMUNOENZYME TECHNIQUES; ISOQUINOLINES; PROLIFERATING CELL NUCLEAR ANTIGEN; TETRAHYDROISOQUINOLINES; TETRAZOLIUM SALTS; TUMOR CELLS, CULTURED; TUMOR SUPPRESSOR PROTEIN P53;

EID: 0035136529     PISSN: 10780432     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (32)

1. Potent antitumor agents from the Caribbean tunicate Ecteinascidia turbinata
2. In vitro antitumor activity of the novel marine agent, ecteinascidin-743 (ET-743, NSC-648766) against human tumors explanted from patients
3. In vitro schedule-dependency of myelotoxicity and cytotoxicity of Ecteinascidin 743 (ET-743)
4. Ecteinascidin-743, a new marine natural product with potent antitumor activity on human ovarian carcinoma xenografts
5. High antitumour activity of ET743 against human tumour xenografts from melanoma, non-small-cell lung and ovarian cancer
6. Final results of Phase I study of ecteinascidin-743 (ET-743) 24 hour continuous infusion in advanced solid tumors patients
7. A phase I and pharmacokinetic trial of ecteinascidin-743 (ET-743) administered as a 72 hour continuous infusion
8. Additional antitumor ecteinascidins from a Caribbean tunicate: Crystal structures and activities in vivo
9. Molecular and crystal structures of ecteinascidins: Potent antitumor compounds from the Caribbean tunicate Ecteinascidia turbinata
10. DNA sequence- and structure-selective alkylation of guanine N2 in the DNA minor groove by ecteinascidin 743, a potent antitumor compound from the Caribbean tunicate, Ecteinascidia Turbinata
11. NMR-based model of an ecteinascidin 743-DNA adduct
12. Poisoning of human DNA topoisomerase I by ecteinascidin 743, an anticancer drug that selectively alkylates DNA in the minor groove
13. Phthalascidin, a synthetic antitumor agent with potency and mode of action comparable to ecteinascidin 743
14. Effect of ecteinascidin-743 on the interaction between DNA binding proteins and DNA
15. Ecteinascidin 743, a transcription-targeted chemotherapeutic that inhibits MDR1 activation
16. Interference of transcriptional activation by the antineoplastic drug ecteinascidin-743
17. Measurement of strand breaks and cross-links by alkaline elution
18. Synergic cell killing by ionizing radiation and topoisomerase I inhibitor topotecan (SK&F 10864)
19. In vitro and in vivo effects of clinically important camptothecin analogues on multidrug-resistant cells
20. Assessment of DNA damage in mammalian cells by DNA filtration methods
21. Qualitative and quantitative aspects of intercalator-induced DNA strand breaks
22. Abrogation of an S-phase checkpoint and potentiation of camptothecin cytotoxicity by 7-hydroxystaurosporine (UCN-01) in human cancer cell lines, possibly influenced by p53
23. Protein-linked, DNA strand breaks induced in mammalian cells by camptothecin, an inhibitor of topoisomerase I
24. Induction of topoisomerase I cleavage complexes by 1-β-D-arabinofuranosylcytosine (Ara-C) in vitro and in ara-C-treated cells
25. Some practical considerations and applications of the National Cancer Institute in vitro anticancer drug discovery screen
26. Prediction of biochemical mechanism of action from the in vitro antitumor screen of the National Cancer Institute
27. Topoisomerase II as a target of anticancer drug action in mammalian cells
28. Mechanism of action of eukaryotic DNA topoisomerase I and drugs targeted to the enzyme
29. DNA topoisomerases
30. Identification of mammalian DNA topoisomerase I as an intracellular target of the anticancer drug camptothecin
31. Molecular basis for the DNA sequence selectivity of ecteinascidin 736 and 743: Evidence for the dominant role of direct readout via hydrogen bonding
32. Ecteinascidin 743: A minor groove alkylator that bends the DNA toward the major groove