Mutational analysis of the structure and functions of hepatitis C virus RNA-dependent RNA polymerase

Hepatology

Volumn 33, Issue 3, 2001, Pages 728-737

  Qin, Weiping ^a   Yamashita, Tatsuya ^a,b   Shirota, Yukihiro ^a,b   Lin, Yong ^a   Wei, Wenxiang ^a   Murakami, Seishi ^a  

^a KANAZAWA UNIVERSITY   (Japan)

^b KANAZAWA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTATHIONE TRANSFERASE; RNA DIRECTED DNA POLYMERASE; RNA POLYMERASE; VIRUS RNA;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CATALYSIS; CELL STRAIN COS1; CRYSTAL STRUCTURE; ENZYME ACTIVITY; GENE MUTATION; HEPATITIS C VIRUS; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION; VIRUS REPLICATION;

EID: 0035120615     PISSN: 02709139     EISSN: None     Source Type: Journal    
DOI: 10.1053/jhep.2001.22765     Document Type: Article

References (48)

1. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome
2. Hepatitis C virus shares amino acid sequence similarity with pestiviruses and flaviviruses as well as members of two plant virus supergroups
3. A proposed system for the nomenclature of hepatitis C viral genotypes
4. The molecular virology of hepatitis C
5. Hepatitis C virus infection is associated with the development of hepatocellular carcinoma
6. Characterization of the hepatitis C virus-encoded serine proteinase: Determination of proteinase-dependent polyprotein cleavage sites
7. Replication of hepatitis C virus
8. Identification and properties of the RNA-dependent RNA polymerase of hepatitis C virus
9. RNA-dependent RNA polymerase of hepatitis C virus
10. Expression of hepatitis C virus NS5B protein: Characterization of its RNA polymerase activity and RNA binding
11. Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity
12. RNA-dependent RNA polymerase activity of the soluble recombinant hepatitis C virus NS5B protein truncated at the C-terminal region
13. Expression, purification, and partial characterization of HCV RNA polymerase
14. A recombinant hepatitis RNA-dependent RNA polymerase capable of copying the full-length viral RNA
15. Polymerase structures and function: Variations on a theme?
16. Crystal structure of the RNA-dependent RNA polymerase from hepatitis C virus reveals a fully encircled active site
17. Identification of four conserved motifs among the RNA-dependent polymerase encoding elements
18. Crystal structure of the RNA-dependent RNA polymerase of hepatitis C virus
19. Identification and properties of the RNA-dependent RNA polymerase of hepatitis C virus
20. Sequence analysis of putative structural regions of hepatitis C virus isolated from 5 Japanese patients with hepatocellular carcinoma
21. Poliovirus RNA synthesis utilizes an RNP complex formed around the 5′-end of viral RNA
22. Complete replication of poliovirus in vitro: Preinitiation RNA replication complexes require soluble cellular factors for the synthesis of VPg-linked RNA
23. Formation of brome mosaic virus RNA-dependent RNA polymerase in yeast requires coexpression of viral proteins and viral RNA
24. Specific interaction between the hepatitis C virus NS5B RNA polymerase and the 3′ end of the viral RNA
25. Bromovirus RNA replication and transcription
26. RNA-protein interactions: Involvement of NS3, NS5, and 3′ noncoding regions of Japanese encephalitis virus genomic RNA
27. Complex formation of NS5B with NS3 and NS4A proteins of hepatitis C virus
28. Association between NS3 and NS5 proteins of dengue virus type 2 in the putative RNA replicase is linked to differential phosphorylation of NS5
29. Assembly of functional Sindbis virus RNA replication complexes: Requirement for coexpression of P123 and P34
30. Polypeptide requirements for assembly of functional Sindbis virus replication complexes: A model for the temporal regulation of minus- and plus-strand RNA synthesis
31. Identification of a region in the Sindbis virus nucleocapsid protein that is involved in specificity of RNA encapsidation
32. The alphaviruses: Gene expression, replication, and evolution
33. NS3 is a serine protease required for processing of hepatitis C virus polyprotein
34. Hepatitis C virus NS5B protein is a membrane-associated phosphoprotein with a predominantly perinuclear localization
35. Structure and organization of the hepatitis C virus genome isolated from human carriers
36. Control of PKR protein kinase by hepatitis C virus nonstructural 5A protein: Molecular mechanism of Kinase regulation
37. Evidence that hepatitis C virus resistance to interferon is mediated through repression of the PKR protein kinease by the nonstructural 5A protein
38. Human hepatitis virus X gene encodes a regulatory domain that represses transactivation of X protein
39. Delineation of regions important for heteromeric association of hepatitis C virus E1 and E2
40. The hepatitis B virus X protein is a co-activator of activated transcription that modulates the transcription machinery and distal binding activators
41. RMP, a novel RNA polymerase subunit 5-interacting protein, counteracts transactivation by hepatitis B virus X protein
42. Suggestion for "safe" residues substitutions in site-directed mutagenesis
43. Site size of cooperative single-stranded RNA binding by poliovirus RNA-dependent RNA polymerase
44. Measurement of poliovirus RNA polymerase binding to poliovirion and nonviral RNAs using a filter-binding assay
45. Crystal structure at 3.5 resolution of HIV-1 reverse transcriptase complexed with an inhibitor
46. Purification and properties of poliovirus RNA polymerase active site
47. Mutational studies of Human DNA polymerase
48. Mutational analysis of bovine viral diarrhea virus RNA-dependent RNA polymerase