메뉴 건너뛰기




Volumn 272, Issue 5263, 1996, Pages 881-884

A U1/U4/U5 snRNP complex induced by a 2′-O-methyl-oligoribonucleotide complementary to U5 snRNA

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; OLIGORIBONUCLEOTIDE; SMALL NUCLEAR RIBONUCLEOPROTEIN; SMALL NUCLEAR RNA;

EID: 0029934852     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5263.881     Document Type: Article
Times cited : (17)

References (39)
  • 1
    • 0001877802 scopus 로고
    • R. F. Gesteland and J. F. Atkins, Eds. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
    • J. M. Moore, C. C. Query, P. A. Sharp, in The RNA World, R. F. Gesteland and J. F. Atkins, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1993), p. 303; P. A. Sharp, Cell 77, 805 (1994); T. W. Nilsen, ibid. 78, 1 (1994).
    • (1993) The RNA World , pp. 303
    • Moore, J.M.1    Query, C.C.2    Sharp, P.A.3
  • 2
    • 0028307171 scopus 로고
    • J. M. Moore, C. C. Query, P. A. Sharp, in The RNA World, R. F. Gesteland and J. F. Atkins, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1993), p. 303; P. A. Sharp, Cell 77, 805 (1994); T. W. Nilsen, ibid. 78, 1 (1994).
    • (1994) Cell , vol.77 , pp. 805
    • Sharp, P.A.1
  • 3
    • 0028308696 scopus 로고
    • J. M. Moore, C. C. Query, P. A. Sharp, in The RNA World, R. F. Gesteland and J. F. Atkins, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1993), p. 303; P. A. Sharp, Cell 77, 805 (1994); T. W. Nilsen, ibid. 78, 1 (1994).
    • (1994) Cell , vol.78 , pp. 1
    • Nilsen, T.W.1
  • 5
    • 0025874112 scopus 로고    scopus 로고
    • B. Seraphin, N. Abovich, M, Rosbash, Nuc. Acids Res. 19, 3857 (1991); G. M. Lamm et al., ibid., p. 3193.
    • Nuc. Acids Res. , pp. 3193
    • Lamm, G.M.1
  • 7
    • 0022894691 scopus 로고
    • B. Chabol, D. L. Black, D. M. LeMaster, J. A. Steitz, Science 230, 1344 (1985); J. Tazi et al., Cell 47, 755 (1986); V. Gerke and J. A. Steitz, ibid., p. 973.
    • (1986) Cell , vol.47 , pp. 755
    • Tazi, J.1
  • 8
    • 0022339325 scopus 로고    scopus 로고
    • B. Chabol, D. L. Black, D. M. LeMaster, J. A. Steitz, Science 230, 1344 (1985); J. Tazi et al., Cell 47, 755 (1986); V. Gerke and J. A. Steitz, ibid., p. 973.
    • Cell , pp. 973
    • Gerke, V.1    Steitz, J.A.2
  • 9
    • 0027059033 scopus 로고
    • J. R. Wyatt, E. J. Sontheimer, J. A. Steitz, Genes Dev. 6, 2542 (1992); A. M. MacMillan et al., ibid. 8, 3008 (1994); S. Teigelkamp, A. J. Newman, J. D. Beggs, EMBO J. 14, 2602 (1995); J. G. Umen and C. Guthrie, Genes Dev. 9, 855 (1995).
    • (1992) Genes Dev. , vol.6 , pp. 2542
    • Wyatt, J.R.1    Sontheimer, E.J.2    Steitz, J.A.3
  • 10
    • 0028556759 scopus 로고
    • J. R. Wyatt, E. J. Sontheimer, J. A. Steitz, Genes Dev. 6, 2542 (1992); A. M. MacMillan et al., ibid. 8, 3008 (1994); S. Teigelkamp, A. J. Newman, J. D. Beggs, EMBO J. 14, 2602 (1995); J. G. Umen and C. Guthrie, Genes Dev. 9, 855 (1995).
    • (1994) Genes Dev. , vol.8 , pp. 3008
    • MacMillan, A.M.1
  • 11
    • 0029031713 scopus 로고
    • J. R. Wyatt, E. J. Sontheimer, J. A. Steitz, Genes Dev. 6, 2542 (1992); A. M. MacMillan et al., ibid. 8, 3008 (1994); S. Teigelkamp, A. J. Newman, J. D. Beggs, EMBO J. 14, 2602 (1995); J. G. Umen and C. Guthrie, Genes Dev. 9, 855 (1995).
    • (1995) EMBO J. , vol.14 , pp. 2602
    • Teigelkamp, S.1    Newman, A.J.2    Beggs, J.D.3
  • 12
    • 0028960809 scopus 로고
    • J. R. Wyatt, E. J. Sontheimer, J. A. Steitz, Genes Dev. 6, 2542 (1992); A. M. MacMillan et al., ibid. 8, 3008 (1994); S. Teigelkamp, A. J. Newman, J. D. Beggs, EMBO J. 14, 2602 (1995); J. G. Umen and C. Guthrie, Genes Dev. 9, 855 (1995).
    • (1995) Genes Dev. , vol.9 , pp. 855
    • Umen, J.G.1    Guthrie, C.2
  • 13
    • 0026663296 scopus 로고
    • J. A. Steitz, Science257, 888 (1992); H. Sawa and J. Abelson, Proc. Natl. Acad. Sci. U.S.A. 89, 11269 (1992); H. Sawa and Y. Shimura, Genes Dev. 6, 244 (1992).
    • (1992) Science , vol.257 , pp. 888
    • Steitz, J.A.1
  • 14
    • 0026453396 scopus 로고
    • J. A. Steitz, Science257, 888 (1992); H. Sawa and J. Abelson, Proc. Natl. Acad. Sci. U.S.A. 89, 11269 (1992); H. Sawa and Y. Shimura, Genes Dev. 6, 244 (1992).
    • (1992) Proc. Natl. Acad. Sci. U.S.A. , vol.89 , pp. 11269
    • Sawa, H.1    Abelson, J.2
  • 15
    • 0026524532 scopus 로고
    • J. A. Steitz, Science257, 888 (1992); H. Sawa and J. Abelson, Proc. Natl. Acad. Sci. U.S.A. 89, 11269 (1992); H. Sawa and Y. Shimura, Genes Dev. 6, 244 (1992).
    • (1992) Genes Dev. , vol.6 , pp. 244
    • Sawa, H.1    Shimura, Y.2
  • 16
    • 0026605043 scopus 로고
    • A. Newman and C. Norman, Cell 68, 743 (1992); E. J. Sontheimer and J. A. Steitz, Science 262, 1989 (1993).
    • (1992) Cell , vol.68 , pp. 743
    • Newman, A.1    Norman, C.2
  • 17
    • 0027739853 scopus 로고
    • A. Newman and C. Norman, Cell 68, 743 (1992); E. J. Sontheimer and J. A. Steitz, Science 262, 1989 (1993).
    • (1993) Science , vol.262 , pp. 1989
    • Sontheimer, E.J.1    Steitz, J.A.2
  • 19
    • 0028016354 scopus 로고
    • B. B. Konforti, M. J. Kcziolkiewicz, M. M. Konarska, Cell 75, 863 (1993); B. B. Konforti and M. M. Konarska, Genes Dev. 8, 1962 (1994).
    • (1994) Genes Dev. , vol.8 , pp. 1962
    • Konforti, B.B.1    Konarska, M.M.2
  • 22
    • 0022386757 scopus 로고
    • D. Solnick, Cell 42, 157 (1985).
    • (1985) Cell , vol.42 , pp. 157
    • Solnick, D.1
  • 25
    • 4243079644 scopus 로고    scopus 로고
    • note
    • 2]. Beads were preblocked with glycogen (0.1 mg/ml), bovine serum albumin (1 mg/ml), and tRNA (0.1 mg/ml) in WB-50 buffer for 20 min at 4°C, then washed four times with 1 ml of WB-50 and once with WB-250 (WB-50 brought to 250 mM NaCl). After resuspension in an equal volume of WB-250, 50-mg aliquots of beads were gently rocked for 1 hour at 4°C with 25 μl of a standard splicing reaction containing 60% v/v HeLa nuclear extract but no substrate or, in later experiments, with either 250 μl of each glycerol gradient fraction. Beads were washed four times with 0.5 ml WB-250 buffer, and bound RNA was purified by digestion with proteinase K, phenol extraction, and ethanol precipitation. Affinity selection on a monomeric avidin column was performed according to the manufacturer (Pierce) with WB-500 as the wash buffer. The three RNA oligonucleotides used in Fig. 4 were 5′-CAGGUAAGUAdT-3′ (5′ SS), 5′-CAUACUUAUUCCdU-3′ (BP), and 5′-CCCUUUUUUCCACAGCUdC-3′ (Py-3′ SS). The sequence of α-Py is 5′-GGAAAAAAGGG-3′.
  • 28
    • 4243191264 scopus 로고    scopus 로고
    • note
    • 2, and 20 mM tris-HCl (pH 7.6) as described (8).
  • 31
    • 0025639012 scopus 로고
    • T. P. Hausner, L. M. Giglio, A. M. Weiner, Genes Dev. 4, 2146 (1990); B. Datta and A. M. Weiner, Nature 352, 821 (1991); J. A. Wu and J. L. Manley, ibid., p. 818.
    • (1990) Genes Dev. , vol.4 , pp. 2146
    • Hausner, T.P.1    Giglio, L.M.2    Weiner, A.M.3
  • 32
    • 0025821210 scopus 로고
    • T. P. Hausner, L. M. Giglio, A. M. Weiner, Genes Dev. 4, 2146 (1990); B. Datta and A. M. Weiner, Nature 352, 821 (1991); J. A. Wu and J. L. Manley, ibid., p. 818.
    • (1991) Nature , vol.352 , pp. 821
    • Datta, B.1    Weiner, A.M.2
  • 33
    • 0025742986 scopus 로고    scopus 로고
    • T. P. Hausner, L. M. Giglio, A. M. Weiner, Genes Dev. 4, 2146 (1990); B. Datta and A. M. Weiner, Nature 352, 821 (1991); J. A. Wu and J. L. Manley, ibid., p. 818.
    • Nature , pp. 818
    • Wu, J.A.1    Manley, J.L.2
  • 34
    • 4243189112 scopus 로고    scopus 로고
    • note
    • Both labeled and unlabeled Py-3′ SS oligo support 5′ SS selection, although labeled oligo is present at 20-fold lower concentration than unlabeled oligo.
  • 35
    • 0027215924 scopus 로고
    • S. Michaud and R. Reed, Genes Dev. 7, 1008 (1993); J. Y. Wu and T. Maniatis, Cell 75, 1061 (1993).
    • (1993) Genes Dev. , vol.7 , pp. 1008
    • Michaud, S.1    Reed, R.2
  • 36
    • 0027137934 scopus 로고
    • S. Michaud and R. Reed, Genes Dev. 7, 1008 (1993); J. Y. Wu and T. Maniatis, Cell 75, 1061 (1993).
    • (1993) Cell , vol.75 , pp. 1061
    • Wu, J.Y.1    Maniatis, T.2
  • 39
    • 4243063746 scopus 로고    scopus 로고
    • note
    • We thank J. A, Steitz for many useful suggestions, oligonucleotides, and Y12 antibody; members of her laboratory for suggestions; A. D. Bailey and T. A. Pavelitz of our laboratory for discussions and critical reading of the manuscript; and M. Moore for the gift of p220 antibody. G.A. was supported by a Human Frontiers Science Program Organization Long-Term Fellowship. This work was funded in part by NIH award GM31073 to A.M.W.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.