Receptor-mediated gene delivery to human peripheral blood mononuclear cells using anti-CD3 antibody coupled to polyethylenimine

Gene Therapy

Volumn 8, Issue 5, 2001, Pages 362-368

  O'Neill, M M ^a   Kennedy, C A ^a   Barton, R W ^a   Tatake, R J ^a  

^a BOEHRINGER INGELHEIM PHARMACEUTICALS INC   (United States)

Author keywords

Gene delivery; Lymphocytes; Nonviral; Receptor mediated

Indexed keywords

CATION; GENE PRODUCT; LIGAND; LYMPHOCYTE ANTIBODY; LYMPHOCYTE ANTIGEN RECEPTOR; PHYTOHEMAGGLUTININ; POLYETHYLENEIMINE; POLYMER;

ARTICLE; CELL ISOLATION; CELL STIMULATION; CONTROLLED STUDY; DRUG DELIVERY SYSTEM; DRUG EFFICACY; ENDOCYTOSIS; FREEZING; GENE EXPRESSION; GENE TARGETING; GENE TRANSFER; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LYMPHOID CELL; MONONUCLEAR CELL; NORMAL HUMAN; PRIORITY JOURNAL; SAMPLE; SIGNAL TRANSDUCTION; TIME; TRANSGENE;

ANTIGENS, CD3; CELL CULTURE TECHNIQUES; GENE TRANSFER TECHNIQUES; HISTOCOMPATIBILITY ANTIGENS CLASS I; HUMANS; INTERCELLULAR ADHESION MOLECULE-1; LEUKOCYTES, MONONUCLEAR; LYMPHOCYTE ACTIVATION; PHYTOHEMAGGLUTININS; PLASMIDS; POLYETHYLENEIMINE; RECEPTORS, IMMUNOLOGIC; TRANSFECTION; TRANSGENES;

EID: 0035086772     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301407     Document Type: Article

References (40)

1. T-cell gene therapy
2. Pathogenic lymphoid cells engineered to express TGF beta 1 ameliorate disease in a collagen-induced arthritis model
3. Molecular analysis of T lymphocyte-directed gene therapy for adenosine deaminase deficiency: Long-term expression in vivo of genes introduced with a retroviral vector
4. HSV-TK gene transfer into donor lymphocytes for control of allogeneic graft-versus-leukemia
5. Human HLA-specific T-cell clones with stable expression of a suicide gene: A possible tool to drive and control a graft-versus-host-graft-versus-leukemia reaction?
6. Efficient transfer of a tumor antigen-reactive TCR to human peripheral blood lymphocytes confers anti-tumor reactivity
7. In vitro transfection of fresh thymocytes and T cells shows subset-specific expression of viral promoters
8. Transcriptional regulation of the interleukin-2 gene in normal human peripheral blood T cells. Convergence of costimulatory signals and differences from transformed T cells
9. T cell activation modulates retrovirus-mediated gene expression
10. Efficient infection of a human T-cell line and of human primary peripheral blood leukocytes with a pseudotyped retrovirus vector
11. Recombinant retroviruses pseudotyped with the vesicular stomatitis virus G glycoprotein mediate both stable gene transfer and pseudotransduction in human peripheral blood lymphocytes
12. Pseudotyping of murine leukemia virus with the envelope glycoproteins of HIV generates a retroviral vector with specificity of infection for CD4-expressing cells
13. + peripheral blood lymphocytes
14. In vivo antitumor effect of cytotoxic T lymphocytes engineered to produce interferon-gamma by adenovirus-mediated genetic transduction
15. Adenoviral-mediated gene transfer in lymphocytes
16. Intra- and extracellular immunization against HIV-1 infection with lymphocytes transduced with an AAV vector expressing a human anti-gp 120 antibody
17. Use of SV40-based vectors to transduce foreign genes to normal human peripheral blood mononuclear cells
18. Transient transfection of murine B lymphocyte blasts as a method for examining gene regulation in primary B cells
19. Consistent transient transfection of DNA into non-transformed human and murine T-lymphocytes
20. Receptor-mediated transport of DNA into eukaryotic cells
21. Receptor-mediated gene delivery employing lectin-binding specificity
22. Delivery of DNA into mammalian cells by receptor-mediated endocytosis and gene therapy
23. Targeted nonviral gene delivery for cancer gene therapy
24. Receptor-mediated gene transfer into human T lymphocytes via binding of DNA/CD3 antibody particles to the CD3 T cell receptor complex
25. Coupling of cell-binding ligands to polyethylenimine for targeted gene delivery
26. A powerful nonviral vector for in vivo gene transfer into the adult mammalian brain: Polyethylenimine
27. Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery
28. Nonviral gene delivery to the rat kidney with polyethylenimine
29. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine
30. In vivo delivery to tumors of DNA complexed with linear polyethylenimine
31. Polyethylenimine-based intravenous delivery of transgenes to mouse lung
32. Polyethylenimine-mediated transfection of human monocytes with the IFN-gamma gene: An approach for cancer adoptive immunotherapy
33. Optimized galenics improve in vitro gene transfer with cationic molecules up to 1000-fold
34. Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells
35. Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery
36. Transferrin-polycation-DNA complexes: The effect of polycations on the structure of the complex and DNA delivery to cells
37. + cells
38. Lymphocyte apoptosis: Induction by gene transfer techniques
39. TNF-alpha secretion and apoptosis of lymphocytes mediated by gene transfer
40. Recognition of an endothelial determinant for CD18-dependent human neutrophil adherence and transendothelial migration