Affinity and location of an internal K+ ion binding site in Shaker K channels

Journal of General Physiology

Volumn 117, Issue 5, 2001, Pages 373-383

  Thompson, Jill ^a   Begenisich, Ted ^a  

^a UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

Author keywords

Ion channels; Ion permeation; Tetraethylammonium; Voltage clamp

Indexed keywords

ION CHANNEL; POTASSIUM; POTASSIUM CHANNEL; TETRYLAMMONIUM;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; BINDING SITE; CHANNEL GATING; COMPETITIVE INHIBITION; CONTROLLED STUDY; ION TRANSPORT; MEMBRANE ELECTROPHYSIOLOGY; NONHUMAN; VOLTAGE CLAMP;

ANIMALS; BINDING SITES; BINDING, COMPETITIVE; ION CHANNEL GATING; MODELS, BIOLOGICAL; OOCYTES; PATCH-CLAMP TECHNIQUES; POTASSIUM; POTASSIUM CHANNELS; PROTEIN STRUCTURE, TERTIARY; SHAKER SUPERFAMILY OF POTASSIUM CHANNELS; TETRAETHYLAMMONIUM; XENOPUS LAEVIS;

EID: 0035030374     PISSN: 00221295     EISSN: None     Source Type: Journal    
DOI: 10.1085/jgp.117.5.373     Document Type: Article

References (37)

1. Almers, W., and E.W. McClesky. 1984. Non-selective conductance in calcium channels of frog muscles: calcium selectivity in a single-file pore. J. Physiol. 353:585-608.
2. +]: a tale of two inactivation mechanisms. Neuron. 15:951-960.
3. + channel. Science. 271:653-656.
4. Begenisich, T., and C. Smith. 1981. Multi-ion nature of potassium channels in squid axons. Curr. Top. Membr. Trans. 22:353-369.
5. Choi, K.L., C. Mossman, C. Aubé, and G. Yellen. 1993. The internal quaternary ammonium receptor site of Shaker potassium channels. Neuron. 10:533-541.
6. Dang, T.X., and E.W. McClesky. 1998. Ion channel selectivity through stepwise changes in binding affinity. J. Gen. Physiol. 111: 185-193.
7. + channel and its structural implications. Nature. 403:321-325.
8. + conduction and selectivity. Science. 280:69-77.
9. Goldin A.L. 1992. Maintenance of Xenopus larvis and oocyte injection. Methods Enzymol. 207:266-279.
10. + channel. Biophys. J. 74:1808-1820.
11. Heginbotham, L., and R. MacKinnon. 1992. The aromatic binding site for tetraethylammonium ion on pottassium channels. Neuron. 8:483-491.
12. + channel. Biophys. J. 65:2089-2096.
13. Hess, P., and R.W. Tsien. 1984. Mechanism of ion permeation through calcium channels. Nature. 309:453-456.
14. Hille, B., and W. Schwarz. 1978. Potassium channels as multi-ion single-file pores. J. Gen. Physiol. 72:409-442.
15. Hoshi, T., W.N. Zagotta, and R.W. Aldrich. 1995. Biophysical and molecular mechanisms of Shaker potassium channel inactivation. Science. 250:533-538.
16. Hurst, R.S., R. Latorre, L. Toro, and E. Stefani. 1995. External barium block of Shaker potassium channels: evidence for two binding sites. J. Gen. Physiol. 106:1069-1087.
17. Hurst, R.S., L. Toro, and E. Stefani. 1996. Molecular determinanats of external barium block in Shaker potassium channels. FEBS Letts. 388:59-65.
18. +-dependent modulation of tetraethylammonium efficacy in Kv2.1 potassium channels. J. Gen. Physiol. 115:509-518.
19. Immke, D., M. Wood, L. Kiss, and S.J. Korn. 1999. Potassium-dependent changes in the comformation of the Kv2.1 potassium channel pore. J. Gen. Physiol. 113:819-838.
20. + channel. Nature. 353:86-90.
21. Jiang, Y., and R. MacKinnon. 2000. the barium site in a potassium channel by X-ray crystallography. J. Gen. Physiol. 115:269-272.
22. + in voltage-gated potassium channels. Evidence for cation binding sites of the different affinity. J. Gen. Physiol. 111:195-206.
23. Newland, C.F., J.P. Adelman, B.L. Tempel, and W. Almers. 1992. Repulsion between tetraethylammonium ions in cloned voltage-gated potassium channels. Neuron. 8:975-982.
24. Neyton, J., and C. Miller. 1988b. Potassium blocks barium permeation through a calcium-activated potassium channel. J. Gen. Physiol. 92:549-568.
25. + channel. J. Gen. Physiol. 92:569-586.
26. + channels. Biophys. J. 66:1929-1938.
27. Slesinger, P.A., Y.N. Jan, and L.Y. Jan. 1993. The S4-S5 loop contributes to the ion-selective pore of potassium channels. Neuron. 11: 739-749.
28. + channel. J. Gen. Physiol. 114:415-426.
29. + fluxes through recombinanat Shaker channels expressed in single Xenopus oocytes. J. Gen. Physiol. 107:449-457.
30. + movement through the pore in IRK1 potassium channels. J. Gen. Physiol. 112:475-484.
31. Starkus, J.G., L. Kuschel, M.D. Rayner, and S.H. Heinemann. 1997. Ion conduction through C-type inactivated Shaker channels. J. Gen. Physiol. 110:539-550.
32. Thompson, J., and T. Begenisich. 2000. Interaction between quaternary ammonium ions in the pore of potassium channels: evidence against an electrostatic mechanism. J. Gen. Physiol. 115: 769-782.
33. + permeability. J. Gen. Physiol. 114:365-376.
34. +-dependent potentiation in Kv2.1 pottasium channels. Biophys. J. 79:2535-2546.
35. Woodhaull, A.M. 1972. Ionic blockage of sodium channels in nerve. J. Gen. Physiol. 61:687-708.
36. + channels by external cations. J. Gen. Physiol. 84:187-199.
37. + channel. Science. 251:939-942.