Green fluorescent protein-cell wall fusion proteins are covalently incorporated into the cell wall of Saccharomyces cerevisiae

FEMS Microbiology Letters

Volumn 162, Issue 2, 1998, Pages 249-255

  Ram, Arthur F J ^a,b   Van Den Ende, Herman ^a   Klis, Frans M ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b LEIDEN UNIVERSITY   (Netherlands)

Author keywords

Cell wall protein; Green fluorescent protein; Protein targeting; Yeast

Indexed keywords

CELL MEMBRANE PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN;

ARTICLE; BACTERIUM CULTURE; CELL WALL; CULTURE MEDIUM; CYTOCHEMISTRY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN METABOLISM; SACCHAROMYCES CEREVISIAE;

BLOTTING, WESTERN; CELL WALL; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MEMBRANE GLYCOPROTEINS; MICROSCOPY, FLUORESCENCE; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

AEQUOREA VICTORIA; BACTERIA (MICROORGANISMS); SACCHAROMYCES CEREVISIAE; VICTORIA;

EID: 0032525141     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(98)00130-X     Document Type: Article

References (17)

1. Klis, F.M. (1994) Review: cell wall assembly in yeast. Yeast 10, 851-869.
2. Cid, V.J., Duran, A., del Rey, F., Snyder, M.P., Nombela, C. and Sanchez, M. (1995) Molecular basis of cell integrity and morphogenesis in S. cerevisiae. Annu. Rev. Microbiol. 59, 345-386.
3. Montijn, R.C., Van Rinsum, J., Van Schagen, F.A. and Klis, F.M. (1994) Glucomannoproteins in the cell wall of S. cerevisiae contain a novel type of carbohydrate side chain. J. Biol. Chem. 269, 19338-19342.
4. Kapteyn, J.C., Montijn, R.C., Vink, E., De La Cruz, J., Llobell, A., Douwes, J.E., Shimoi, H., Lipke, P.N. and Klis, F.M. (1996) Retention of S. cerevisiae cell wall proteins through a phosphodiester-linked β-1,3-/β-1,6-glucan heteropolymer. Glycobiology 6, 337-345.
5. Caro, L.H.P., Tettelin, H., Vossen, J.H., Ram, A.F.J., van den Ende, H. and Klis, F.M. (1997) In silico identification of glycosyl-phosphatidyl inositol-anchored plasma-membrane and cell wall proteins of S. cerevisiae. Yeast 13, 1477-1489.
6. Kollar, R., Reinhold, B.B., Petrakova, E., Yeh, H.J.C., Ashwell, G., Drgonova, J., Kapteyn, J.C., Klis, F.M. and Cabib, E. (1997) Architecture of the yeast cell wall: β(1-6)-glucan interconnects mannoproteins. J. Biol. Chem. 272, 17762-17775.
7. De Nobel, J.G. and Lipke, P.N. (1994) Is there a role for GPIs in yeast cell wall assembly? Trends Cell Biol. 4, 41-45.
8. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W.W. and Prasher, D.C. (1994) Green fluorescent protein as a marker for gene expression. Science 263, 802-804.
9. Winston, F., Dollard, C. and Ricupero-Hovasse, S.L. (1995) Construction of a set of convenient S. cerevisiae strains that are isogenic to S288C. Yeast 11, 53-55.
10. Imai, J., Toh-e, A. and Matsui, Y. (1996) Genetic analysis of the S. cerevisiae RHO3 gene, encoding a rho-type small GTPase, provides evidence for a role in bud formation. Genetics 142, 359-369.
11. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
12. Ito, H., Fukuda, Y., Murata, M. and Kimura, A. (1983) Transformations of intact yeast cells with alkali cations. J. Bacteriol. 153, 163-168.
13. Horton, R.M., Hunt, H.D., Ho, S.N., Hullen, J.K. and Pease, L.R. (1988) Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77, 61-68.
14. Gietz, R.D. and Sugino, A. (1988) New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74, 527-534.
15. Ozols, J. (1990) Amino acid analysis. Methods Enzymol. 182, 587-601.
16. Ram, A.F.J., Wolters, A., ten Hoopen, R. and Klis, F.M. (1994) A new approach for isolating cell wall mutants in S. cerevisiae by screening for hypersensitivity to Calcofluor White. Yeast 10, 1019-1030.
17. Tkacz, J.S. and Lampen, J.O. (1972) Wall replication in Saccharomyces species: use of fluorescein-conjugated concanavalin A to reveal the site of mannan insertion. J. Gen. Microbiol. 72, 243-247.