In vivo dynamics of nuclear pore complexes in yeast

Journal of Cell Biology

Volumn 136, Issue 6, 1997, Pages 1185-1199

  Bucci, Mirella ^a   Wente, Susan R ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL NUCLEUS; CELL NUCLEUS MEMBRANE; DYNAMICS; INTRACELLULAR TRANSPORT; LIPID BILAYER; NONHUMAN; PRIORITY JOURNAL; REGULATORY MECHANISM; YEAST;

BIOLOGICAL TRANSPORT; FUNGAL PROTEINS; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MACROMOLECULAR SUBSTANCES; MEMBRANE FUSION; MOVEMENT; NUCLEAR ENVELOPE; NUCLEAR PROTEINS; RECOMBINANT FUSION PROTEINS; REPRODUCTION; SACCHAROMYCES CEREVISIAE;

AEQUOREA VICTORIA; SACCHAROMYCES CEREVISIAE; VICTORIA;

EID: 0030793799     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.136.6.1185     Document Type: Article

References (76)

1. Aitchison, J.D., G. Blobel, and M.P. Rout. 1995a. Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport. J. Cell Biol. 131:1659-1675.
2. Aitchison, J.D., M.P. Rout, M. Marelli, G. Blobel, and R.W. Wozniak. 1995b. Two novel related yeast nucleoporins Nup170p and Nup157p : complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p. J. Cell Biol. 131: 1133-1148.
3. Akey, C.W. 1995. Structural plasticity of the nuclear pore complex. J. Mol. Biol. 248:273-293.
4. Aris, J.P., and G. Blobel. 1989. Yeast nuclear envelope proteins cross-react with an antibody against mammalian pore complex proteins. J. Cell Biol. 108: 2059-2067.
5. Baudin, A., K.O. Ozier, A. Denouel, F. Lacroute, and C. Cullin. 1993. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucleic Acids Res. 21:3329-3330.
6. Boman, A.L., M.R. Delannoy, and K.L. Wilson. 1992a. GTP hydrolysis is required for vesicle fusion during nuclear envelope assembly in vitro. J. Cell Biol. 116:281-294.
7. Boman, A.L., T.C. Taylor, P. Melancon, and K.L. Wilson. 1992b. A role for ADP-ribosylation factor in nuclear vesicle dynamics. Nature (Lond.). 358: 512-514.
8. Christianson, T.W., R.S. Sikorski, M. Dante, J.H. Shero, and P. Hieter. 1992. Multifunctional yeast high-copy-number shuttle vectors. Gene (Amst.). 110: 119-122.
9. Conde, J., and G.R. Fink. 1976. A mutant of Saccharomyces cerevisiae defective for nuclear fusion. Proc. Natl. Acad. Sci. USA. 73:3651-3655.
10. Dabauvalle, M.C., K. Loos, H. Merkert, and U. Scheer. 1991. Spontaneous assembly of pore complex-containing membranes ("annulate lamellae") in Xenopus egg extract in the absence of chromatin. J. Cell Biol. 112:1073-1082.
11. Dabauvalle, M.C., K. Loos, and U. Scheer. 1990. Identification of a soluble precursor complex essential for nuclear pore assembly in vitro. Chromosoma (Berl.). 100:56-66.
12. Davis, L.I. 1995. The nuclear pore complex. Annu. Rev. Biochem. 64:865-896.
13. + RNA transport and nuclear pore distribution. EMBO (Eur. Mol. Biol. Organ.) J. 13:6062-6075.
14. Fawcett, D.W. 1981. The Cell. Second edition. W.B. Saunders Company, Philadelphia.
15. Finlay, D.R., and D.J. Forbes. 1990. Reconstitution of biochemically altered nuclear pores: transport can be eliminated and restored. Cell. 60:17-29.
16. Finlay, D.R., E. Meier, P. Bradley, J. Horecka, and D.J. Forbes. 1991. A complex of nuclear pore proteins required for pore function. J. Cell Biol. 114: 169-183.
17. Forbes, D.J. 1992. Structure and function of the nuclear pore complex. Annu. Rev. Cell Biol. 8:495-527.
18. Gerace, L., and R. Foisner. 1994. Integral membrane proteins and dynamic organization of the nuclear envelope. Trends Cell Biol. 4:127-131.
19. Goldberg, M.W., and T.D. Allen. 1995. Structural and functional organization of the nuclear envelope. Curr. Opin. Cell Biol. 7:301-309.
20. Goldstein, A.L., C.A. Snay, C.V. Heath, and C.N. Cole. 1996. Pleiotropic nuclear defects associated with a conditional allele of the novel nucleoporin Rat9p/Nup85p. Mol. Biol. Cell. 7:917-934.
21. Gorlich, D., and I.W. Mattaj. 1996. Nucleocytoplasmic transport. Science (Wash. DC). 271:1513-1518.
22. Gorsch, L.C., T.C. Dockendorff, and C.N. Cole. 1995. A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J. Cell Biol. 129:939-955.
23. Grandi, P., V. Doye, and E.C. Hurt. 1993. Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96. EMBO (Eur. Mol. Biol. Organ.) J. 12:3061-3071.
24. Hampton, R.Y., A. Koning, R. Wright, and J. Rine. 1996. In vivo examination of membrane protein localization and degradation with green fluorescent protein. Proc. Natl. Acad. Sci. USA. 93:828-833.
25. + RNA associated with mutation of the Saccharomyces cerevisiae rat2/nup120 gene. J. Cell Biol. 131:1677-1697.
26. Heim, R., and R.Y. Tsien. 1996. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr. Biol. 6:178-182.
27. Iovine, M.K., J.L. Watkins, and S.R. Wente. 1995. The GLFG repetitive region of the nucleoporin Nup116p interacts with Kap95p, an essential veast nuclear import factor. J. Cell Biol. 131:1699-1713.
28. Ito, H., Y. Fukuda, K. Murata, and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163-168.
29. Jordan, E.G., N.J. Severs, and D.H. Williamson. 1977. Nuclear pore formation and the cell cycle in Saccharomytes cerevisiae. Exp. Cell Res. 104:446-449.
30. Kahana, J.A., B.J. Schnapp, and P.A Silver. 1995. Kinetics of spindle pole body separation in budding yeast. Proc. Natl. Acad. Sci. USA. 92:9707-9711.
31. Kessel, R.G. 1983. The structure and function of annulate lamellae: porous cytoplasmic and intranuclear membranes. Int. Rev. Cytol. 82:181-303.
32. Kurihara, L.J., C.T. Beh, M. Latterich, R. Schekman, and M.D. Rose. 1994. Nuclear congression and membrane fusion: two distinct events in the yeast karyogamy pathway. J. Cell Biol. 126:911-923.
33. Latterich, M., and R. Schekman. 1994. The karyogamy gene KAR2 and novel proteins are required for ER-membrane fusion. Cell. 78:87-98.
34. + RNA and constitutive clustering of nuclear pore complexes. Mol. Biol. Cell. 6:401-417.
35. Lohka, M.J., and Y. Masui. 1983. Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components. Science (Wash. DC). 220:719-721.
36. Macaulay, C., and D.J. Forbes. 1996. Assembly of the nuclear pore: biochemically distinct steps revealed with NEM, GTPγS, and BAPTA. J. Cell Biol. 132:5-20.
37. Maul, G.G. 1977. The nuclear and the cytoplasmic pore complex: structure, dynamics, distribution, and evolution. Int. Rev. Cytol. (Suppl.). 5:75-186.
38. Meier, E., B.R. Miller, and D.J. Forbes. 1995. Nuclear pore complex assembly studied with a biochemical assay for annulate lamellae formation. J. Cell Biol. 129:1459-1472.
39. Melchior, F., and L. Gerace. 1995. Mechanisms of nuclear protein import. Curr. Opin. Cell Biol. 7:310-318.
40. Meluh, P.B., and M.D. Rose. 1990. KAR3, a kinesin-related gene required for yeast nuclear fusion. Cell. 60:1029-1041.
41. Murphy, R., J.L. Watkins, and S.R. Wente. 1996. GLE2, a S. cerevisiae homologue of the S. pombe export factor RAE1, is required for nuclear pore complex structure and function. Mol. Biol. Cell. 7:1921-1937.
42. Mutvei, A., S. Dihlmann, W. Herth, and E.C. Hurt. 1992. NSP1 depletion in yeast affects nuclear pore formation and nuclear accumulation. Eur. J. Cell Biol. 59:280-295.
43. Nehrbass, U., H. Kern, A. Mutvei, H. Horstmann, B. Marshallsay, and E.C. Hurt. 1990. NSP1: a yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain. Cell. 61: 979-989.
44. Newmeyer, D.D., and D.J. Forbes. 1990. A N-ethylmaleimide-sensitive cytosolic factor necessary for nuclear protein import: requirement in signal-mediated binding to the nuclear pore. J. Cell Biol. 110:547-557.
45. Newport, J. 1987. Nuclear reconstitution in vitro: stages of assembly around protein-free DNA. Cell. 48:205-217.
46. Newport, J.W., and W. Dunphy. 1992. Characterization of the membrane binding and fusion events during nuclear envelope assembly using purified components. J Cell Biol. 116:295-306.
47. Osborne, M.A., and P.A. Silver. 1993. Nucleocytoplasmic transport in the yeast Saccharomyces cerevisiae. Annu. Rev. Biochem. 62:219-254.
48. Pante, N., and U. Aebi. 1996. Molecular dissection of the nuclear pore complex. Crit. Rev. Biochem. Mol. Biol. 31:153-199.
49. Ponberton, L.F., M.P. Rout, and G. Blobel. 1995. Disruption of the nucleoporin gene NUP133 results in clustering of nuclear pore complexes. Proc. Natl. Acad. Sci. USA. 92:1187-1191.
50. Pfaller, R., C. Smythe, and J.W. Newport. 1991. Assembly/disassembly of the nuclear envelope membrane: cell cycle-dependent binding of nuclear membrane vesicles to chromatin in vitro. Cell. 65:209-217.
51. Ris, H. 1991. The three dimensional structure of the nuclear pore complex as seen by high voltage electron microscopy and high resolution low voltage scanning electron microscopy. EMSA Bull. 21:54-56.
52. Rose, M.D., and G.R. Fink. 1987. KAR1, a gene required for function of both intranuclear and extranuclear microtubules in yeast. Cell. 48:1047-1060.
53. Rout, M.P., and G. Blobel. 1993. Isolation of the yeast nuclear pore complex. J. Cell Biol. 123:771-783.
54. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Second edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 545 pp.
55. Severs, N.J., E.G. Jordan, and D.H. Williamson. 1976. Nuclear pore absence from areas of close association between nucleus and vacuole in synchronous yeast cultures. J. Ultrastruct. Res. 54:374-387.
56. Sheehan, M.A., A.D. Mills, A.M. Sleeman, R.A. Laskey, and J.J. Blow. 1988. Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopus eggs. J. Cell Biol. 106:1-12.
57. Sherman, F., G.R. Fink, and J.B. Hicks. 1986. Methods in Yeast Genetics. Vol. 35. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
58. Sikorski, R.S., and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 122:19-27.
59. Siniossoglou, S., C. Wimmer, M. Rieger, V. Doye, H. Tekotte, C. Weise, S. Emig, A. Segref, and E.C. Hurt. 1996. A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores. Cell. 84:265-275.
60. Smith, S., and G. Blobel. 1994. Colocalization of vertebrate lamin B and lamin B receptor (LBR) in nuclear envelopes and in LBR-induced membrane stacks of the yeast Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA. 91: 10124-10128.
61. Stearns, T. 1995. Green fluorescent protein. The green revolution. Curr. Biol. 5: 262-264.
62. Stewart, M. 1992. Nuclear pore structure and function. Semin. Cell Biol. 3:267-277.
63. Sullivan, K.M., W.B. Busa, and K.L. Wilson. 1993. Calcium mobilization is required for nuclear vesicle fusion in vitro: implications for membrane traffic and IP3 receptor function. Cell. 73:1411-1422.
64. Underwood, M.R., and H.M. Fried. 1990. Characterization of nuclear localizing sequences derived from yeast ribosomal protein L29. EMBO (Eur. Mol. Biol. Organ.) J. 9:91-99.
65. Vallen, E.A., M.A. Hiller, T.Y. Scherson, and M.D. Rose. 1992. Separate domains of KAR1 mediate distinct functions in mitosis and nuclear fusion. J. Cell Biol. 117:1277-1287.
66. Vigers, G.P., and M.J. Lohka. 1991. A distinct vesicle population targets membranes and pore complexes to the nuclear envelope in Xenopus eggs. J. Cell Biol. 112:545-556.
67. Vigers, G P., and M.J. Lohka. 1992. Regulation of nuclear envelope precursor functions during cell division. J. Cell Sci. 102:273-284.
68. Waddle, J.A., T.S. Karpova, R.H. Waterston, and J.A. Cooper. 1996. Movement of cortical actin patches in yeast. J. Cell Biol. 132:861-870.
69. Wente, S.R., and G. Blobel. 1993. A temperature-sensitive NUP116 null mutant forms a nuclear envelope seal over the yeast nuclear pore complex thereby blocking nucleocytoplasmic traffic. J. Cell Biol. 123:275-284.
70. Wente, S.R., and G. Blobel. 1994. NUP145 encodes a novel yeast glycine-leucine-phenylalanine-glycine (GLFG) nucleoporin required for nuclear envelope structure. J. Cell Biol. 125:955-969.
71. Wente, S.R., S.M. Gasser, and A.J. Caplan. 1996. The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae. In The Molecular and Cellular Biology of the Yeast Saccharomyces. Vol. 3. J.R. Broach, E. Jones, and J. Pringle, editors. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. In press.
72. Wente, S.R., M.P. Rout, and G. Blobel. 1992. A new family of yeast nuclear pore complex proteins. J. Cell Biol. 119:705-723.
73. Wiese, C., and K.L. Wilson. 1993. Nuclear membrane dynamics. Curr. Opin. Cell Biol. 5:387-394.
74. Wright, R., M. Basson, L. D'Ari, and J. Rine. 1988. Increased amounts of HMG-CoA reductase induce "karmellae": a proliferation of stacked membrane pairs surrounding the yeast nucleus. J. Cell Biol. 107:101-114.
75. Yeh, E., R.V. Skibbens, J.W. Cheng, E.D. Salmon, and K. Bloom. 1995. Spindle dynamics and cell cycle regulation of dynein in the budding yeast, Saccharomyces cerevisiae. J. Cell Biol. 130:687-700.
76. Zabel, U., V. Doye, H. Tekotte, R. Wepf, P. Grandi, and E.C. Hurt. 1996. Nic96p is required for nuclear pore formation and functionally interacts with a novel nucleoporin, Nup188p. J. Cell Biol. 133:1141-1152.