Optimization and optimality of a short ribozyme ligase that joins non-Watson-Crick base pairings

RNA

Volumn 7, Issue 4, 2001, Pages 513-523

  Robertson, M P ^a   Hesselberth, J R ^a   Ellington, A D ^a  

^a The University of Texas at Austin   (United States)

Author keywords

Ligase; Ribozyme

Indexed keywords

LIGASE; RIBOZYME;

ARTICLE; BASE PAIRING; ENZYME ACTIVITY; ENZYME STRUCTURE; HUMAN; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; SEQUENCE ANALYSIS;

BASE PAIRING; BASE SEQUENCE; CATALYSIS; DIRECTED MOLECULAR EVOLUTION; LIGASES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; RNA, CATALYTIC; SUBSTRATE SPECIFICITY;

EID: 0035000517     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838201002199     Document Type: Article

References (31)

1. Isolation of new ribozymes from a large pool of random sequences
2. A sparse matrix approach to crystallizing ribozymes and RNA motifs
3. Isolation of a ribozyme with 5′-5′ ligase activity
4. In vitro selection of nucleic acid aptamers that bind proteins
5. A DNA metalloenzyme with DNA ligase activity
6. The secondary structure and sequence optimization of an RNA ligase ribozyme
7. Structurally complex and highly active RNA ligases derived from random RNA sequences
8. Ribozymes in wonderland
9. A general module for RNA crystallization
10. Crystals by design: A strategy for crystallization of a ribozyme derived from the Tetrahymena group I intron
11. Isolation of novel ribozymes that ligate AMP-activated RNA substrates
12. In vitro selection of nucleic acids for diagnostic applications
13. Structural features that give rise to the unusual stability of RNA hairpins containing GNRA loops
14. The new world of ribozymes
15. Allosteric selection of ribozymes that respond to the second messengers cGMP and cAMP
16. Artificial evolution and natural ribozymes
17. Emergence of a dual-catalytic RNA with metal-specific cleavage and ligase activities: The spandrels of RNA evolution
18. Capping DNA with DNA
19. Design, synthesis, and amplification of DNA pools for in vitro selection
20. Ribozymes: Red in tooth and caw
21. In vitro selection of an allosteric ribozyme that transduces analytes to amplicons
22. Ribozyme selection
23. Design and optimization of effector-activated ribozyme ligases
24. A ribozyme that lacks cytidine
25. Nucleic acid molecular switches
26. Allosteric nucleic acid catalysts
27. Examination of the catalytic fitness of the hammerhead ribozyme by in vitro selection
28. Isolation of hammerhead ribozymes with altered core sequences by in vitro selection
29. Purification, crystallization, and X-ray diffraction analysis of small ribozymes
30. In vitro selection of functional nucleic acids
31. Continuous in vitro evolution of catalytic function