The use of 7-amino actinomycin D in identifying apoptosis: Simplicity of use and broad spectrum of application compared with other techniques

Blood

Volumn 87, Issue 6, 1996, Pages 2244-2251

  Philpott, N J ^a   Turner, A J C ^a   Scopes, J ^a   Westby, M ^a   Marsh, J C W ^a   Gordon Smith, E C ^a   Dalgleish, A G ^a   Gibson, F M ^a  

^a ST GEORGE'S UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DACTINOMYCIN;

APOPTOSIS; ARTICLE; CELL DIFFERENTIATION; CELL PROLIFERATION; DRUG USE; HEMATOLOGIC DISEASE; HUMAN; HUMAN CELL; PATHOGENESIS; PRIORITY JOURNAL;

EID: 0029866409     PISSN: 00064971     EISSN: None     Source Type: Journal    
DOI: 10.1182/blood.v87.6.2244.bloodjournal8762244     Document Type: Article

References (36)

1. Williams GT, Smith CA, Spooncer E, Dexter TM, Taylor DR: Haemopoietic colony stimulating factors promote cell survival by suppressing apoptosis. Nature 343:76, 1990
2. Harrington EA, Fanidi A, Evan GI: Oncogenes and cell death. Curr Opin Genet Dev 4:120, 1994
3. Maciejewski J, Selleri C, Anderson S, Young NS: Fas antigen expression on CD34+ human marrow cells is induced by interferon-γ and rumor necrosis factor- α and potentiates cytokine-mediated hematopoietic suppression in vitro. Blood 85:3183, 1995
4. Kerr JFR, Winterford CM, Harmon BV: Apoptosis: Its significance in cancer and cancer therapy. Cancer 73:2013, 1994
5. Dive C, Hickman JA: Drug-target interactions: Only the first step in the commitment to a programmed cell death. Br J Cancer 64:192, 1991
6. Amiesen JC: Programmed cell death and AIDS: From hypothesis to experiment. Immunol Today 13:388, 1992
7. Banda NK, Bernier J, Kurahara DK, Kurrle R, Haigwood N, Sekaly R-P, Finkel TH: Crosslinking CD4 by human immunodeficiency virus gp120 primes T cells for activation induced apoptosis. J Exp Med 176:1099, 1992
8. Kerr JFR, Wyllie AH, Currie AR: Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26:239, 1972
9. Wyllie AH, Kerr JF, Currie AR: Cell death: Significance of apoptosis. Int Rev Cytol 68:251, 1980
10. Arends M, Wyllie AH: Apoptosis: Mechanisms and roles in pathology. Int Rev Exp Pathol 32:223, 1991
11. Wyllie AH: Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 284:555, 1980
12. Arends MJ, Morris RG, Wyllie AH: Apoptosis: The role of the endonuclease. Am J Pathol 136:593, 1990
13. Harrington EA, Bennett MR, Fanidi A, Evan GI: c-myc-induced apoptosis in fibroblasts is inhibited by specific cytokines. EMBO 13:3286, 1994
14. Wyllie AH, Morris RG, Smith AL, Dunlop D: Chromatin cleavage in apoptosis: Association with condensed chromatin morphology and dependence on macromolecular synthesis. J Pathol 142:67, 1984
15. Evan GI, Wyllie AH, Gilbert CS, Littlewood TD, Land H, Brooks M, Waters CM, Penn LZ, Hancock DC: Induction of apoptosis in fibroblasts by c-myc protein. Cell 69:119, 1992
16. Duke RC, Chervenak R, Cohen JJ: Endogenous endonuclease-induced DNA fragmentation: An early event in cell-mediated cytolysis. Proc Natl Acad Sci USA 80:6361, 1983
17. Kawabata H, Anzai N, Yoshida Y, Okuma M: A new method for quantitative estimation of the degree of DNA fragmentation utilising agarose gel electrophoresis. Int J Hematol 59:311, 1994
18. Kulkarni GV, McCulloch CAG: Serum deprivation induces apoptotic cell death in a subset of Balb/c 3T3 fibroblasts. J Cell Sci 107:1169, 1994
19. Cohen GM, Sun XM, Snowden RT, Dinsdale D, Skilleter DN: Key morphological features of apoptosis may occur in the absence of internucleosomal DNA fragmentation. Biochem J 286:331, 1992
20. Cohen JJ: Apoptosis: Physiologic cell death. J Lab Clin Med 124:761, 1994
21. Schmid I, Uittenbogaart C, Giorgi J: Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometry. Cytometry 15:12, 1994
22. Schmid I, Uittenbogaart CH, Keld B, Giorgi JV: A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry. J Immunol Methods 170:145, 1994
23. Chomczynski P: A reagent for the single step isolation of RNA, DNA and protein from cell and tissue samples. Biotechniques 15:532, 1993
24. Nicolleti I, Migliorati G, Pagliacci MC, Griganani F, Riccardi C: A rapid and simple method for measuring thymocyte apoptosis by propidtum iodide staining and flow cytometry. J Immunol Methods 139:271, 1992
25. Huschtscha LI, Jeitner TM, Andersson CE, Bartier WA, Tattersall MH: Identification of apoptotic and necrotic human leukaemic cells by flow cytometry. Exp Cell Res 212:161, 1994
26. Pellicciari C, Manfredi AA, Bottone MG, Schaack V, Barni S: A single-step staining procedure for the detection and sorting of unfixed apoptotic thymocytes. Eur J Histochem 37:381, 1993
27. Telford WG, King LE, Fraker PJ: Comparative evaluation of several DNA binding dyes in the detection of apoptosis-associated chromatin degradation by flow cytometry. Cytometry 13:137, 1992
28. Zamai L, Falcieri E, Zauli G, Cataldi A, Vitale M: Optimal detection of apoptosis by flow cytometry depends on cell morphology. Cytometry 14:891, 1993
29. McConkey DJ, Hartzell P, Amador-Perez JF, Orrenius S, Jondal M: Calcium-dependent killing of immature thymocytes by stimulation via the CD3/T cell receptor complex. J Immunol 143:1801, 1989
30. Ormerod MG, Collins MKL, Rodriguez-Tarduchy G, Robertson D: Apoptosis in interleukin-3 dependent haemopoietic cells. Quantification by two flow cytometric methods J Immunol Methods 153:57, 1992
31. Hardin JA, Sherr DH, DeMaria M, Lopez PA: A simple fluorescence method for surface antigen phenotyping of lymphocytes undergoing DNA fragmentation. J Immunol Methods 154:99, 1992
32. Gavrieli Y, Sherman Y, Ben-Sasson SA: Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 119:493, 1992
33. Hotz MA, Gong J, Traganos F, Darzynkiewicz Z: Flow cytometric detection of apoptosis: Comparison of the assays of in situ DNA degradation and chromatin changes. Cytometry 15:237, 1994
34. Gorczyca W, Gong J, Darzynkiewicz Z: Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res 53:1945, 1993
35. Gorczyca W, Melamed MR, Darzynkiewicz Z: Apoptosis of S-phase HL60 cells induced by DNA topoisomerase inhibitors: Detection of DNA strand breaks by flow cytometry using the in situ nick translation assay. Toxicol Lett 67:249, 1993
36. Muller W, Crothers DM: Studies of the binding of actinomycin and related compounds to DNA. J Mol Biol 35:251, 1975